1. Circulating microRNA-200 Family as Diagnostic Marker in Hepatocellular Carcinoma
- Author
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Iyad Kabar, Jörg Haier, Norbert Senninger, Anna Hüsing, Heiner Wolters, Sameer A. Dhayat, and Hartmut Schmidt
- Subjects
Oncology ,Adult ,Liver Cirrhosis ,Male ,medicine.medical_specialty ,Alcoholic liver disease ,Cirrhosis ,Carcinoma, Hepatocellular ,lcsh:Medicine ,Gastroenterology ,Diagnosis, Differential ,Young Adult ,Blood serum ,Internal medicine ,medicine ,Carcinoma ,Biomarkers, Tumor ,Humans ,lcsh:Science ,Aged ,Multidisciplinary ,business.industry ,lcsh:R ,Liver Neoplasms ,Case-control study ,Middle Aged ,medicine.disease ,digestive system diseases ,Circulating MicroRNA ,MicroRNAs ,ROC Curve ,Hepatocellular carcinoma ,Case-Control Studies ,lcsh:Q ,Female ,Differential diagnosis ,business ,Research Article - Abstract
Goals In this clinical study, we aimed to evaluate the role of circulating microRNA-200 family as a non-invasive tool to identify patients with cirrhosis-associated hepatocellular carcinoma (HCC). Background Prognosis of HCC remains poor with increasing incidence worldwide, mainly related to liver cirrhosis. So far, no reliable molecular targets exist for early detection of HCC at surgically manageable stages. Recently, we identified members of the microRNA-200 family as potential diagnostic markers of cirrhosis-associated HCC in patient tissue samples. Their value as circulating biomarkers for HCC remained undefined. Methods Blood samples and clinicopathological data of consecutive patients with liver diseases were collected prospectively. Expression of the microRNA-200 family was investigated by qRT-PCR in blood serum samples of 22 HCC patients with and without cirrhosis. Serum samples of patients with non-cancerous chronic liver cirrhosis (n = 22) and of healthy volunteers (n = 15) served as controls. Results MicroRNA-141 and microRNA-200a were significantly downregulated in blood serum of patients with HCC compared to liver cirrhosis (p
- Published
- 2015