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Experimental evolution (EE) is a powerful research framework for gaining insights into many biological questions, including the evolution of reproductive systems. We designed a long-term and highly replicated EE project using the nematode C. elegans, with the main aim of investigating the impact of reproductive system on adaptation and diversification under environmental challenge. From the laboratory-adapted strain N2, we derived isogenic lines and introgressed the fog-2(q71) mutation, which changes the reproductive system from nearly exclusive selfing to obligatory outcrossing, independently into 3 of them. This way, we obtained 3 pairs of isogenic ancestral populations differing in reproductive system; from these, we derived replicate EE populations and let them evolve in either novel (increased temperature) or control conditions for over 100 generations. Subsequently, fitness of both EE and ancestral populations was assayed under the increased temperature conditions. Importantly, each population was assayed in 2–4 independent blocks, allowing us to gain insight into the reproducibility of fitness scores. We expected to find upward fitness divergence, compared to ancestors, in populations which had evolved in this treatment, particularly in the outcrossing ones due to the benefits of genetic shuffling. However, our data did not support these predictions. The first major finding was very strong effect of replicate block on populations' fitness scores. This indicates that despite standardization procedures, some important environmental effects were varying among blocks, and possibly compounded by epigenetic inheritance. Our second key finding was that patterns of EE populations' divergence from ancestors differed among the ancestral isolines, suggesting that research conclusions derived for any particular genetic background should never be generalized without sampling a wider set of backgrounds. Overall, our results support the calls to pay more attention to biological variability when designing studies and interpreting their results, and to avoid over-generalizations of outcomes obtained for specific genetic and/or environmental conditions. [ABSTRACT FROM AUTHOR]

Global warming and invasive species, separately or combined, can impose a large impact on the condition of native species. However, we know relatively little about how these two factors, individually and in combination, shape phenotypes in ectotherms across life stages and how this can differ between populations. We investigated the non-consumptive predator effects (NCEs) imposed by native (perch) and invasive (signal crayfish) predators experienced only during the egg stage or during both the egg and larval stages in combination with warming on adult life history traits of the damselfly Ischnura elegans. To explore microgeographic differentiation, we compared two nearby populations differing in thermal conditions and predator history. In the absence of predator cues, warming positively affected damselfly survival, possibly because the warmer temperature was closer to the optimal temperature. In the presence of predator cues, warming decreased survival, indicating a synergistic effect of these two variables on survival. In one population, predator cues from perch led to increased survival, especially under the current temperature, likely because of predator stress acclimation phenomena. While warming decreased, predator cues increased larval development time with a proportionally stronger effect of signal crayfish cues experienced during the egg stage, indicating a negative carry-over effect from egg to larva. Warming and predator cues increased mass at emergence, with the predator effect driven mainly by exposure to signal crayfish cues during the egg stage, indicating a positive carry-over effect from egg to adult. Notably, warming and predator effects were not consistent across the two studied populations, suggesting a phenotypic signal of adaptation at a microgeographic scale to thermal conditions and predator history. We also observed pronounced shifts during ontogeny from synergistic (egg and early larval stage) toward additive (late larval stage up to emergence) effects between warming and predator stress. The results point out that population- and life-stage-specific responses in life-history traits to NCEs are needed to predict fitness consequences of exposure to native and invasive predators and warming in prey at a microgeographic scale. [ABSTRACT FROM AUTHOR]

Environmental temperatures and oxygen availability are important for the balance between oxygen supply and demand. Terrestrial organisms are generally perceived to be less limited by access to oxygen than their aquatic counterparts. Nevertheless, even terrestrial environments can be deficient in oxygen, especially for organisms occurring in soil, litter, wood, rotten fruit or at high elevations. While isopods are the best adapted to a terrestrial lifestyle among crustaceans, many species, including woodlice, occupy environmental gradients of temperature and oxygen. To investigate whether mismatches between oxygen supply and demand can result in a loss of performance in a terrestrial organism, we studied the effects of atmospheric oxygen concentration on the thermal performance of the common rough woodlouse (Porcellio scaber). We compared the thermal preference, thermal sensitivity of running speed, and tolerance to extreme temperatures of woodlice exposed to one of two oxygen concentrations (21% - normoxia, 7% - hypoxia). Under hypoxia, P. scaber preferred microhabitats with temperatures that were on average 3°C lower than those preferred under normoxia. The running speed tended to reach its maximum at a lower temperature under hypoxia than under normoxia (25.13°C vs 28.87°C, respectively, although p was equal to 0.09), and normoxic woodlice ran approximately 1.5-fold faster than hypoxic woodlice at the point of maximum speed. Heat tolerance was significantly lower under hypoxia (38.9°C) than under normoxia (40.7°C), but there was no difference in cold tolerance (5.81°C under normoxia and 5.44°C under hypoxia). Overall, our results indicate that environmental gradients of temperature and oxygen may shape the physiological performance of terrestrial ectotherms, likely via their effects on the balance between oxygen supply and demand, which may have fitness consequences for these organisms in nature. [ABSTRACT FROM AUTHOR]