1. Magnetic ligand fishing using immobilized DPP-IV for identification of antidiabetic ligands in lingonberry extract.
- Author
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Lima, Rita de Cássia Lemos, Böcker, Ulrike, McDougall, Gordon J., Allwood, J. William, Afseth, Nils Kristian, and Wubshet, Sileshi Gizachew
- Subjects
PEPTIDASE ,CD26 antigen ,LIGANDS (Biochemistry) ,HYPOGLYCEMIC agents ,TYPE 2 diabetes ,FISHING ,GLUCOSIDES - Abstract
In this work, a new magnetic ligand fishing probe for discovery of DPP-IV inhibitory ligands was developed and it was tested as a proof of concept on the fruit extract of Vaccinium vitis-idaea (lingonberry). The ligands were shown to have appreciable dipeptidyl peptidase IV (DPP-IV) inhibitory activity (IC
50 : 31.8 μg mL-1).) Inhibition of DPP-IV is a well-known therapeutic approach for management of type 2 diabetes (T2D). DPP-IV was successfully immobilized onto magnetic beads and was shown to retain its catalytic activity and selectivity over a model mixture. A total of four ligands were successfully fished out and identified as cyanidin-3-galactoside (2), cyanidin-3-arabinoside (3), proanthocynidin A (4), and 10-carboxyl-pyranopeonidin 3-O-(6″-O-p-coumaroyl)-glucoside (5) using HPLC/HRMS. [ABSTRACT FROM AUTHOR]- Published
- 2021
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