1. Casein kinase Iɛ in the Wnt pathway: Regulation of β-catenin function
- Author
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Lewis T. Williams, Chie Sakanaka, Licen Xu, Stephen D. Harrison, and Peng Leong
- Subjects
chemistry.chemical_classification ,Multidisciplinary ,Beta-catenin ,biology ,Xenopus ,Wnt signaling pathway ,Casein Kinase Iepsilon ,biology.organism_classification ,Dishevelled ,chemistry ,Cancer research ,biology.protein ,Casein kinase 1 ,Signal transduction ,Casein kinases - Abstract
Wnt and its intracellular effector β-catenin regulate developmental and oncogenic processes. Using expression cloning to identify novel components of the Wnt pathway, we isolated casein kinase Iɛ (CKIɛ). CKIɛ mimicked Wnt in inducing a secondary axis in Xenopus , stabilizing β-catenin, and stimulating gene transcription in cells. Inhibition of endogenous CKIɛ by kinase-defective CKIɛ or CKIɛ antisense-oligonucleotides attenuated Wnt signaling. CKIɛ was in a complex with axin and other downstream components of the Wnt pathway, including Dishevelled. CKIɛ appears to be a positive regulator of the pathway and a link between upstream signals and the complexes that regulate β-catenin.
- Published
- 1999
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