1. Translation and developmental regulation of RNA encoded by the eukaryotic transposable element copia
- Author
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Gerald M. Rubin, John J. Toole, Bryan E. Roberts, Stephanie W. Ruby, and Andrew J. Flavell
- Subjects
Transposable element ,Transcription, Genetic ,Biology ,Restriction fragment ,chemistry.chemical_compound ,Reticulocyte ,medicine ,Animals ,RNA, Messenger ,Genetics ,Multidisciplinary ,fungi ,Intron ,Proteins ,RNA ,Translation (biology) ,Molecular Weight ,RNA silencing ,Drosophila melanogaster ,medicine.anatomical_structure ,Genes ,chemistry ,Biochemistry ,Protein Biosynthesis ,DNA Transposable Elements ,biology.protein ,DNA ,Research Article - Abstract
copia-specific RNA was isolated from Drosophila melanogaster tissue culture cells by hybridization of cytoplasmic polyadenylylated RNA to copia DNA immobilized on cellulose. The purified RNA was translated in reticulocyte lysates. One major polypeptide of approximately 51,000 daltons was synthesized in addition to several others between 18,000 and 38,000 daltons. The 51,000-dalton polypeptide and several of the others are encoded by mRNAs of about 2000 nucleotides. The approximate locations on the copia element of the coding sequences for the 51,000-dalton polypeptide and several other proteins were determined by hybrid-arrested translation with copia restriction fragments. The relative abundance of copia-specific RNA was determined at various stages of the Drosophila life cycle. The level of copia-specific RNA is modulated during development of the organism, with the highest level occurring during the larval stages.
- Published
- 1980
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