1. Expression of EhRAD54, EhRAD51, and EhBLM proteins during DNA repair by homologous recombination in Entamoeba histolytica.
- Author
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Charcas-Lopez Mdel S, Garcia-Morales L, Pezet-Valdez M, Lopez-Camarillo C, Zamorano-Carrillo A, and Marchat LA
- Subjects
- Amino Acid Sequence, Animals, Cell Nucleus chemistry, Consensus Sequence, Cytoplasm chemistry, DNA Breaks, Double-Stranded, DNA Helicases chemistry, DNA Helicases genetics, DNA Helicases physiology, DNA, Protozoan genetics, DNA, Protozoan radiation effects, Entamoeba histolytica genetics, Entamoeba histolytica radiation effects, Genes, Protozoan, Humans, Models, Molecular, Molecular Sequence Data, Protein Conformation, Protozoan Proteins chemistry, Protozoan Proteins genetics, Rad51 Recombinase genetics, Rad51 Recombinase physiology, RecQ Helicases genetics, RecQ Helicases physiology, Sequence Alignment, Sequence Homology, Amino Acid, Time Factors, Ultraviolet Rays, DNA Repair genetics, Entamoeba histolytica metabolism, Homologous Recombination genetics, Protozoan Proteins physiology
- Abstract
Entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events. It contains the RAD52 epistasis group genes, including Ehrad51 and Ehrad54, and the Ehblm gene, which are key homologous recombination factors in other organisms. Ehrad51 and Ehrad54 genes are differentially transcribed in trophozoites when DNA double-strand breaks are induced by ultraviolet-C irradiation. Moreover, the EhRAD51 recombinase is overexpressed at 30 min in the nucleus. Here, we extend our analysis of the homologous recombination mechanism in E. histolytica by studying EhRAD51, EhRAD54, and EhBLM expression in response to DNA damage. Bioinformatic analyses show that EhRAD54 has the molecular features of homologous proteins, indicating that it may have similar functions. Western blot assays evidence the differential expression of EhRAD51, EhRAD54, and EhBLM at different times after DNA damage, suggesting their potential roles in the different steps of homologous recombination in this protozoan., (© Ma. del Socorro Charcas-Lopez et al., published by EDP Sciences, 2014.)
- Published
- 2014
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