1. Effects of proinsulin C-peptide on nitric oxide, microvascular blood flow and erythrocyte Na+,K+-ATPase activity in diabetes mellitus type I
- Author
-
Philippe Vague, B. L. Johansson, K. Goitom, T. Pohlmann, S. Schneider, J. Beyer, M. Löbig, Thomas Forst, D. Dufayet De La Tour, T. Kunt, A. Pfützner, M. Engelbach, and John Wahren
- Subjects
medicine.medical_specialty ,C-peptide ,Arbitrary unit ,Nitric Oxide Synthase Type III ,Venous blood ,General Medicine ,Nitric oxide ,chemistry.chemical_compound ,Red blood cell ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Internal medicine ,medicine ,Acetylcholine ,medicine.drug ,Proinsulin - Abstract
This study was conducted to evaluate the influence of proinsulin C-peptide on erythrocyte Na(+),K(+)-ATPase and endothelial nitric oxide synthase activities in patients with type I diabetes. In a randomized double-blind study design, ten patients with type I diabetes received intravenous infusions of either human C-peptide or physiological saline on two different occasions. C-peptide was infused at a rate of 3 pmol.min(-1).kg(-1) for 60 min, and thereafter at 10 pmol.min(-1).kg(-1) for 60 min. At baseline and after 60 and 120 min, laser Doppler flow (LDF) was measured following acetylcholine iontophoresis or mild thermal stimulation (44 degrees C), and venous blood samples were collected to determine plasma cGMP levels and erythrocyte membrane Na(+),K(+)-ATPase activity. The LDF response to acetylcholine increased during C-peptide infusion and decreased during saline infusion [18.6+/-19.2 and -13.2+/-9.4 arbitrary units respectively; mean+/-S.E.M.; P
- Published
- 2000
- Full Text
- View/download PDF