1. Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies
- Author
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Xinyu Wen, Pengjun Zhang, Xingwang Jia, Liyuan Tian, Jing Gao, Wei Run, Yaping Tian, Junhao Gui, Wen-hui Zhang, and Yanhong Gao
- Subjects
Liver Cirrhosis ,Male ,Pathology ,Cirrhosis ,AST, aspartate aminotransferase ,Polymerase Chain Reaction ,RNA, Neoplasm ,NC, normal control ,microRNA ,AFP, α-fetoprotein ,RT, reverse transcription ,Liver Diseases ,Liver Neoplasms ,General Medicine ,Middle Aged ,Up-Regulation ,Real-time polymerase chain reaction ,Mamm U6, mammalian U6 ,Hepatocellular carcinoma ,biomarker ,GC, gastric cancer ,Biomarker (medicine) ,Female ,CHB, chronic hepatitis B ,ICC, intrahepatic cholangiocellular carcinoma ,Research Article ,Adult ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,liver pathology ,Biology ,S3 ,GGT, γ-glutamyl transpeptidase ,ALT, alanine aminotransferase ,snRNA, small nuclear RNA ,Biomarkers, Tumor ,medicine ,TaqMan ,Humans ,miRNAs, microRNAs ,Gene silencing ,miR-885-5p ,FNH, focal nodular hyperplasia ,Aged ,Gene Expression Profiling ,cirrhosis ,Cancer ,LC, liver cirrhosis ,medicine.disease ,RT-preamp-qPCR, RT-preamplification-qPCR ,digestive system diseases ,ROC, receiver operating characteristic ,MicroRNAs ,AUC, the area under the ROC curve ,Liver function ,qPCR, quantitative PCR ,Epidemiologic Methods ,HCC, hepatocellular carcinoma ,serum ,Biomarkers - Abstract
Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first employed to profile miRNAs in serum pools from patients with HCC (hepatocellular carcinoma) or LC (liver cirrhosis) and from healthy controls. Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls. The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed. The levels of miR-885-5p were significantly higher in sera from patients with HCC, LC and CHB than in healthy controls or GC patients. miR-885-5p yielded an AUC [the area under the ROC (receiver operating characteristic) curve] of 0.904 [95% CI (confidence interval), 0.837–0.951, P
- Published
- 2010