1. Adenomatous polyposis coli protein (APC)-independent regulation of β-catenin/Tcf-4 mediated transcription in intestinal cells
- Author
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Antonio García de Herreros, Josep Baulida, and Eduard Batlle
- Subjects
Transcriptional activity ,Beta-catenin ,Cell Biology ,Biology ,Biochemistry ,Molecular biology ,In vitro ,Cytosol ,medicine.anatomical_structure ,Transcription (biology) ,medicine ,biology.protein ,Adenomatous Polyposis Coli Protein ,Molecular Biology ,Nucleus ,Protein kinase C - Abstract
Alterations in the transcriptional activity of the beta-catenin-Tcf complex have been associated with the earlier stages of colonic transformation. We show here that the activation of protein kinase C by the phorbol ester PMA in several intestinal cell lines increases the levels of beta-catenin detected in the nucleus and augments the transcriptional activity mediated by beta-catenin. The response to PMA was not related to modifications in the cytosolic levels of beta-catenin and was observed not only in cells with wild-type adenomatous polyposis coli protein (APC) but also in APC-deficient cells. Binding assays in vitro revealed that PMA facilitates the interaction of the beta-catenin with the nuclear structure. Our results therefore show that beta-catenin-mediated transcription can be regulated independently of the presence of APC.
- Published
- 1999
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