1. Emodin ameliorates renal injury in BXSB mice by modulating TNF-α/ICAM-1
- Author
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Enqin Lin, Beiduo Lin, Binbin Dai, Liyan Yang, Xinlu Yuan, and Yangbin Pan
- Subjects
Male ,medicine.medical_specialty ,Small interfering RNA ,Emodin ,ICAM-1 ,Kidney Glomerulus ,Biophysics ,Lupus nephritis ,Administration, Oral ,Cell Death & Injury ,Biochemistry ,Cell Line ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Humans ,RNA, Small Interfering ,skin and connective tissue diseases ,Molecular Biology ,Research Articles ,lupus nephritis ,030203 arthritis & rheumatology ,Systemic lupus erythematosus ,biology ,Tumor Necrosis Factor-alpha ,Chemistry ,Cell Biology ,Intercellular Adhesion Molecule-1 ,medicine.disease ,Gastrointestinal, Renal & Hepatic Systems ,Therapeutics & Molecular Medicine ,Signaling ,Disease Models, Animal ,Endocrinology ,Mechanism of action ,TNF-α ,030220 oncology & carcinogenesis ,Cell Transdifferentiation ,biology.protein ,Tumor necrosis factor alpha ,Antibody ,medicine.symptom ,Signal Transduction - Abstract
The purpose of the present study was to explore the effects of emodin on renal injury in a BXSB mouse model of lupus and its mechanisms. BXSB mice were fed different concentrations of emodin (0, 5, 10 and 20 mg/kg.d), and the levels of intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-α (TNF-α) and fibronectin (FN) levels in the glomeruli and serum levels of the anti-dsDNA antibody were determined. Mesangial cells (MCs) were cultured in vitro, and IgG-type anti-dsDNA antibody and/or emodin were added to the MC culture supernatant. In addition, TNF-α small interfering RNA (siRNA) was transfected into MCs to explore the mechanism of action of emodin. The results showed that the mice fed emodin presented decreases in the urinary protein content and glomerular TNF-α, ICAM-1 and FN levels (P
- Published
- 2020
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