1. Distinct higher-order alpha-synuclein oligomers induce intracellular aggretation
- Author
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David Smith, Roberto Cappai, Eva Illes-Toth, Mafalda Ribeiro Ramos, and Caroline F. Dalton
- Subjects
Models, Molecular ,Spectrometry, Mass, Electrospray Ionization ,Cell Survival ,Protein Conformation ,Electrospray ionization ,Expert Systems ,Protein aggregation ,Tandem mass spectrometry ,Fibril ,Protein Aggregation, Pathological ,Biochemistry ,Oligomer ,chemistry.chemical_compound ,Protein structure ,Tandem Mass Spectrometry ,Cell Line, Tumor ,Humans ,Calcium Signaling ,Molecular Biology ,Neurons ,Alpha-synuclein ,Computational Biology ,Cell Biology ,Recombinant Proteins ,Intrinsically Disordered Proteins ,Molecular Weight ,chemistry ,alpha-Synuclein ,Intracellular - Abstract
Misfolding and aggregation of alpha-synuclein (α-syn) into Lewy bodies (LB) is associated with a range of neurological disorders, including Parkinson's disease (PD). The cell to cell transmission of α-syn pathology has been linked to soluble amyloid oligomer populations that preceded LB formation. Oligomers produced in vitro under certain conditions have been demonstrated to induce intracellular aggregation in cell culture models. Here we characterize, by electrospray ionisation - ion mobility spectrometry - mass spectrometry (ESI-IMS-MS), a specific population of α-syn oligomers. These mass spectrometry compatible oligomers were compared with oligomers with known seeding and pore forming capabilities and were shown to have the ability to induce intracellular aggregation. Each oligomer type was shown to have distinct epitope profiles that correlated with their toxic gain of function. Structurally the mass spectrometry compatible oligomers populated a range of species from dimers through to hexamers. Lower order oligomers were structurally diverse and consistent with unstructured assemblies. Higher order oligomers were shown to be compact with ring-like structures. The observation of this compact state may explain how this natively disordered protein is able to transfer pathology from cell to cell and avoid degradation by cellular proteases.
- Published
- 2015