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Your search keyword '"Hiroshi Araya"' showing total 2 results
Start Over Author "Hiroshi Araya" Publisher pharmaceutical society of japan
2 results on '"Hiroshi Araya"'

1. Squamosten-A, a Novel Mono-tetrahydrofuranic Acetogenin with a Double Bond in the Hydrocarbon Chain, from Annona aquamosa L

Author

Anjani Srivastava, Yoshinori Fujimoto, Mahendra Sahai, Hiroshi Araya, and Noriyuki Hara

Subjects
chemistry.chemical_classification, biology, Double bond, Stereochemistry, General Chemistry, General Medicine, Annona squamosa, Pharmacognosy, biology.organism_classification, chemistry.chemical_compound, Hydrocarbon, chemistry, Chain (algebraic topology), Annonaceae, Drug Discovery, Acetogenin
Published
1994

2. Multiple forms of aryl sulfotransferase for acetaminophen sulfate conjugation in rat liver cytosol

Author

Hiroshi Araya, Shoji Awazu, Takashi Mizuma, and Masahiro Hayashi

Subjects
In Vitro Techniques, Michaelis–Menten kinetics, Nitrophenols, Cytosol, Sulfation, Drug Stability, medicine, Animals, Thermolabile, Acetaminophen, Pharmacology, chemistry.chemical_classification, Aryl sulfotransferase, fungi, digestive, oral, and skin physiology, Temperature, food and beverages, Rats, Inbred Strains, Metabolism, Hydrogen-Ion Concentration, Arylsulfotransferase, Rats, Isoenzymes, Kinetics, Enzyme, Liver, chemistry, Biochemistry, Sulfurtransferases, medicine.drug
Abstract

The multiplicity of aryl sulfotransferase (phenol sulfotransferase, PST) in acetaminophen (APAP) sulfate conjugation was studied in rat liver cytosol. The sulfation rate showed the optimal pH of about 9 similar to that in the phenolic monoamine sulfation. And another optimal pH of about 6.4 was found at the higher APAP concentration such as 16 mM, suggesting the existence of the PST multiplicity in the APAP sulfation. The effect of thermal treatment at various temperatures, 37 to 41 degrees C, showed that PST catalyzing the sulfation at the lower APAP concentration (about less than 1 mM) is more thermolabile and has the lower Km for APAP than at the higher APAP concentration. The APAP sulfation at microM order APAP in the presence of p-nitrophenol (PNP) was shown to be decreased by the substrate inhibition of PNP to PST. Consequently it is considered the sulfation at the lower APAP concentration (microM order) is mainly catalyzed by the thermolabile PST with the lower Km for APAP, and at the higher APAP concentration the thermostable PST with the higher Km partially contributes to the sulfation.

Published
1984

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