1. Voltage-dependent calcium channel subtypes controlling somatic substance P release in the peripheral nervous system.
- Author
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Harding LM, Beadle DJ, and Bermudez I
- Subjects
- Animals, Calcium Channel Blockers pharmacology, Calcium Channels drug effects, Calcium Channels, L-Type drug effects, Calcium Channels, N-Type drug effects, Calcium Channels, P-Type drug effects, Electrophysiology, Female, Ganglia, Spinal cytology, Ganglia, Spinal metabolism, Ganglia, Spinal ultrastructure, Immunohistochemistry, In Vitro Techniques, Ion Channel Gating drug effects, Male, Microscopy, Electron, Neurons drug effects, Neurons metabolism, Neurons ultrastructure, Peripheral Nervous System drug effects, Potassium pharmacology, Rats, Rats, Sprague-Dawley, Calcium Channels physiology, Ion Channel Gating physiology, Peripheral Nervous System metabolism, Substance P metabolism
- Abstract
1. Isolated rat dorsal root ganglia (DRG) neurones support vesicular, non synaptic release of substance P in a depolarisation and Ca2+ dependent manner. 2. In vivo this process may mediate cross-communication between DRG cells in some neuropathological conditions and is therefore a putative area for drug intervention. 3. The authors investigated the voltage-dependent Ca2+ channel (VDCC) subtypes involved in somatic release of substance P. Fresh (< 1 day) cultures of DRG neurones were incubated with high K+ depolarising saline in the presence and absence of subtype selective VDCC blockers. Substance P released into the external media was collected and quantified using a radioimmunoassay. 4. The results show that L-type and N-type, but not P-type, VDCCs play an important role in high K+ evoked substance P release from rat DRG neurones.
- Published
- 1999
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