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The European Commission developed an amendment to Regulation 691/2011 on European environmental economic accounts to include reporting on ecosystem accounts compliant to the United Nations Statistical Commission System of Environmental-Economic Accounts – Ecosystem Accounts (SEEA-EA) standard. To support Member States implementing this regulation, an open source tool, known as INCA-tool, to generate ecosystem service accounts has been developed, based on the Knowledge Innovation Project on Integrated Systems of Naural Capital and Ecosystem Services Accounting (KIP-INCA) methodologies. The INCA-tool was developed by taking into account the FAIR principle for software and data, as well as existing interoperability standards by the SEEA community. Three types of users were identified with their specific needs, interactions and skills. To meet their needs, the INCA-tool was split into two parts, a python package to perform the calculations and an acessible and easy-to-use user interface in QGIS to integrate national information. With a first version of the toolkit in place, improvements to the existing calculation methods and alignment with the upcoming EU regulation can be achieved. Further, feedback from Member States beta-tests and their experiences is currently collected and implemented and the full public roll-out is planned for the end of 2022. The software packages in the toolkit were already used to extend the existing nine INCA European wall-to-wall account series with the year 2018.