1. The effect of pathogenesis-related 10 (Pr-10) gene on the progression of fusarium wilt in Musa acuminata cv. Berangan
- Author
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Baharum, Nadiya Akmal, Othman, Rofina Yasmin, Mohd Yusuf, Yusmin, tan, Boon Chin, Zaidi, Kamilatulhusna, Khalid, Norzulaani, Baharum, Nadiya Akmal, Othman, Rofina Yasmin, Mohd Yusuf, Yusmin, tan, Boon Chin, Zaidi, Kamilatulhusna, and Khalid, Norzulaani
- Abstract
PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over- expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants.
- Published
- 2018