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Background: NAC (NAM, ATAF1/2, and CUC2) transcription factors play an important role in plant growth and development. However, in tumorous stem mustard ( Brassica juncea var. tumida ), one of the economically important crops cultivated in southwest China and some southeast Asian countries, reports on the identification of NAC family genes are lacking. In this study, we conducted a genome-wide investigation of the NAC family genes in B . juncea var. tumida , based on its recently published genome sequence data., Methods: The NAC genes were identified in B . juncea var. tumida using the bioinformatics approach on the whole genome level. Additionally, the expression of BjuNAC genes was analyzed under high- and low-temperature stresses by quantitative real-time PCR (qRT-PCR)., Results: A total of 300 BjuNAC genes were identified, of which 278 were mapped to specific chromosomes. Phylogenetic analysis of B . juncea var. tumida , Brassica rapa , Brassica nigra , rice and Arabidopsis thaliana NAC proteins revealed that all NAC genes were divided into 18 subgroups. Furthermore, gene structure analysis showed that most of the NAC genes contained two or three exons. Conserved motif analysis revealed that BjuNAC genes contain a conserved NAM domain. Additionally, qRT-PCR data indicated that thirteen BjuNAC genes with a varying degree of up-regulation during high-temperature stress. Conversely, four BjuNAC genes ( BjuNAC006 , BjuNAC083 , BjuNAC170 and BjuNAC223 ) were up-regulated and two BjuNAC genes ( BjuNAC074 and BjuNAC295 ) down-regulated under low temperature, respectively. Together, the results of this study provide a strong foundation for future investigation of the biological function of NAC genes in B . juncea var. tumida ., Competing Interests: The authors declare there are no competing interests., (©2021 Jiang et al.)