1. The inhibitory influence of calix[4]Arene of C-90 on the activity of Ca2+,Mg2+-ATPases in plasma membrane and sarcoplasmic reticulum in myometrium cells.
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Veklich TO
- Subjects
- Animals, Ca(2+) Mg(2+)-ATPase metabolism, Calcium metabolism, Cell Membrane enzymology, Cell Size, Female, Ion Transport drug effects, Kinetics, Myocytes, Smooth Muscle enzymology, Myometrium drug effects, Myometrium enzymology, Oxytocin pharmacology, Protein Binding, Sarcoplasmic Reticulum enzymology, Swine, Ca(2+) Mg(2+)-ATPase antagonists & inhibitors, Calixarenes pharmacology, Cell Membrane drug effects, Enzyme Inhibitors pharmacology, Myocytes, Smooth Muscle drug effects, Phenols pharmacology, Sarcoplasmic Reticulum drug effects
- Abstract
Our study on the plasma membrane vesicles and myometrium cells treated with 0.1% digitonin showed that inhibitory effect of calix[4]arene C-90 (5,11,17,23-tetra(trifluoro)methyl(phenylsulphonylimino)-methylamino- 25,26,27,28-tetrapropoxy-calix[4]arene) on the plasma membrane Ca2+,Mg2+-ATPase was more significant than on the Ca2+,Mg2+-ATPase in sarcoplasmic reticulum (the inhibition coefficient I0.5 values were 20.2 ± 0.5 μM and 57.0 ± 1.4 μM for the plasma membrane Ca2+,Mg2+-ATPase and Ca2+,Mg2+-ATPase in sarcoplasmic reticulum, respectively (n = 5)). Inhibition kinetics of calix[4]arene C-90 effect on the Ca2+,Mg2+- ATPase activities in plasma membrane and sarcoplasmic reticulum were studied. This substance inhibited both pumps as complete noncompetitive inhibitor. Calix[4]arene C-90 caused the increase of intracellular Ca2+ concentration and decrease of hydrodynamic diameter in smooth muscle cells similar to the action of uterotonic drug oxytocin.
- Published
- 2016
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