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3. Localization of beta-defensin genes in non human primates.

Author

Ventura M, Boniotto M, Pazienza M, Palumbo V, Cardone MF, Rocchi M, Tossi A, Amoroso A, and Crovella S

Subjects
Animals, Cell Line, Haplorhini, Hominidae, Humans, In Situ Hybridization, Fluorescence methods, Chromosome Mapping, Primates genetics, beta-Defensins genetics
Abstract

Defensins are a family of host defence peptides that play an important role in the innate immunity of mammalian and avian species. In humans, four beta-defensins have been isolated so far, corresponding to the products of the genes DEFB1 (h-BD1, GenBank accession number NM_005218); DEFB4 (h-Bd2, NM_004942.2), DEFB103 (h-BD3, NM_018661); and DEFB104 (hBD4, NM_080389) mapping on chromosome 8p23.22. We have localized beta-defensin genes on metaphasic chromosomes of great apes and several non-human primate species to determine their physical mapping. Using fluorescent in situ hybridization and BAC probes containing the four beta-defensin genes, we have mapped the homologous regions to the beta-defensin genes on chromosome 8p23-p.22 in non-human primates, while no signals were detected on prosimians chromosomes.

Published
2004

4. Detection of MBL-2 gene expression in intestinal biopsies of celiac patients by in situ reverse transcription polymerase chain reaction.

Author

Boniotto M, Radillo O, Braida L, Pirulli D, Città A, Not T, Amoroso A, and Crovella S

Subjects
Biopsy, Celiac Disease metabolism, Celiac Disease pathology, DNA Primers chemistry, DNA, Complementary metabolism, Gene Expression, Humans, Intestine, Small metabolism, Intestine, Small pathology, Mannose-Binding Lectin metabolism, RNA, Messenger metabolism, Celiac Disease genetics, Mannose-Binding Lectin analogs & derivatives, Mannose-Binding Lectin genetics, Reverse Transcriptase Polymerase Chain Reaction methods
Published
2003
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5. Localization and expression of two human b-defensins (HBD-1 and HBD-2) in intestinal biopsies of celiac patients.

Author

Boniotto M, Pirulli D, Verga Falzacappa MV, Trevisiol C, Gerarduzzi T, and Crovella S

Subjects
Biopsy, Celiac Disease pathology, Duodenum pathology, Gene Expression, Humans, RNA, Messenger genetics, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, beta-Defensins genetics, Celiac Disease metabolism, Duodenum metabolism, beta-Defensins metabolism
Published
2003

6. Fluorescent in situ PCR allows sensitive three hours detection of human papilloma virus in cells and tissues.

Author

Marziliano N, Mancuso T, Comar M, Cernigoi E, Da Prato L, Garagna S, Boniotto M, Pirulli D, Puzzer D, Morgutti M, Amoroso A, and Crovella S

Subjects
Fluorescein-5-isothiocyanate, HeLa Cells, Humans, Papillomavirus Infections pathology, Sensitivity and Specificity, Time Factors, Tumor Virus Infections pathology, Papillomaviridae isolation & purification, Papillomavirus Infections virology, Primed In Situ Labeling methods, Tumor Virus Infections virology
Published
1999

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