1. [Decrease of insulin binding to human acute leukemic cells during chemically induced differentiation].
- Author
-
Palumbo A, Turco GL, Brossa C, D'Alberto M, Ghezzo F, and Pegoraro L
- Subjects
- Cell Line, Dimethyl Sulfoxide pharmacology, Granulocytes cytology, Humans, Leukemia, Myeloid metabolism, Macrophages cytology, Macrophages metabolism, Receptor, Insulin metabolism, Tetradecanoylphorbol Acetate pharmacology, Tretinoin pharmacology, Cell Differentiation drug effects, Granulocytes metabolism, Insulin metabolism, Leukemia, Myeloid, Acute metabolism
- Abstract
The specific insulin binding activity of human promyelocitic HL 60 cell line during the myeloid and macrophagic differentiation induced by chemical compounds was investigated. Dimethyl sulfoxide (DMSO) and retinoic acid myeloid induced differentiation in HHL 60 cells was accompanied by a marked decrease of insulin receptors. In K 562 cell line, where DMS O has no effect on differentiation, the number of insulin receptors was only slightly affected. 12-0-tetradodecanoil phorbol 13-acetate (TPA) induced macrophagic differentiation of HL 60 cell line was also accompanied by a decrease of insulin binding activity. Our results support the hypothesis that during the process of terminal differentiation a decrease of insulin receptors occurs.
- Published
- 1980