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5. [Effect of calcium and other cations on pyruvate transport in rat liver mitochondria].

Author

Paradies G and Papa S

Subjects
Animals, Biological Transport, Active drug effects, Cations, Divalent, Cinnamates pharmacology, Hydrogen-Ion Concentration, Kinetics, Mitochondria, Liver drug effects, Monocarboxylic Acid Transporters, Nitriles pharmacology, Oxygen Consumption, Rats, Calcium pharmacology, Mitochondria, Liver metabolism, Pyruvates metabolism
Abstract

The effect of Ca2+ and other cations on the initial rate of pyruvate translocation by rat liver mitochondria has been studied. It has been found that added Ca2+ strongly stimulates the rate of pyruvate uptake by non-respiring mitochondria. Mn2+ and to lesser extent Sr2+ could replace Ca2+ whilst Mg2+ has only little effect. The stimulation of pyruvate uptake by Ca2+ is completely abolished by alpha-cyanocinnamate a specific inhibitor of pyruvate translocation but not by lanthanide which inhibits Ca2+ uptake. Direct measurements of the transmembrane delta pH in mitochondria show no appreciable changes following the addition of Ca2+, this excluding the possibility that the Ca2+ dependent stimulation of pyruvate uptake might be due to an increase of transmembrane delta pH. It is therefore conceivable that the effect of Ca2+ can be ascribed to an interaction of this cation with the translocator on the external of the mitochondrial membrane. Although the data obtained do not warrant postulation of the nature of the calcium induced stimulation of mitochondrial pyruvate transport it is likely that this effect may be of a great importance in the regulation of pyruvate transport and metabolism in mitochondria.

Published
1980

6. [Analysis of cellular components in immunocompetent organs treated with ionizing radiations].

Author

Bonora B, Bartoli S, Santi P, Antonucci A, Palka G, Papa S, and Mazzotti G

Subjects
Animals, B-Lymphocytes metabolism, B-Lymphocytes radiation effects, Bursa of Fabricius cytology, Bursa of Fabricius metabolism, Chickens, DNA metabolism, Lysosomes metabolism, Mitosis radiation effects, RNA metabolism, T-Lymphocytes metabolism, T-Lymphocytes radiation effects, Thymus Gland cytology, Thymus Gland metabolism, Bursa of Fabricius radiation effects, Thymus Gland radiation effects
Abstract

The nucleic acid and protein content has been evaluated in lymphoid cells from the thymus and the Bursa of Fabricius of chicks exposed to different doses of radiation. A different response to radiation damage of the two cell populations is suggested by these analysis when compared with ultrastructural data.

Published
1979

9. [Effects of ionizing radiations on the ultrastructure of the thymus gland].

Author

Mazzotti G, Mosca Bubola G, Pettazzoni P, Ruggeri F, Santi P, Papa S, and Farulla A

Subjects
Animals, Chickens, Chromatin radiation effects, Epithelial Cells, Lymphocyte Activation radiation effects, Lymphocytes radiation effects, Macrophages radiation effects, Mitochondria radiation effects, Thymus Gland ultrastructure, Time Factors, Thymus Gland radiation effects
Abstract

Different doses of gamma rays induce ultrastructural alterations in limphoid and epithelial cells of the chick thymus which exhibit a different temporal evolution after the treatment. The main changes which occur in lymphoid cells treated with 50 rads disappear 48 hours after the irradiation, while with 100 rads the radiation damage is still evident in epithelial cells.

Published
1979

10. [Effect of ortho-phenylphenol on mitochondrial respiration].

Author

Cheah KS, Boffoli D, Guerrieri F, and Papa S

Subjects
Animals, Cattle, Male, Mitochondria physiology, Myocardium enzymology, Succinate Dehydrogenase metabolism, Mitochondria drug effects, Oxygen Consumption drug effects, Phenols pharmacology
Published
1976

14. [Dependence of the proton redox pup pH on mitochondria].

Author

Papa S, Guerrieri F, Izzo G, Boffoli D, and Capuano F

Subjects
Animals, Cattle, Cytochrome c Group metabolism, Heart drug effects, Hydrogen-Ion Concentration, Male, Nigericin pharmacology, Oxidation-Reduction drug effects, Oxygen Consumption drug effects, Quinones pharmacology, Mitochondria drug effects, Protons
Published
1976

18. [Metabolism of glutamic acid in brain mitochondria].

Author

Lofrumento NE, De Gregorio G, Papa S, Serra C, and Quagliariello E

Subjects
Animals, Rats, Brain metabolism, Glutamates metabolism, Glutamine metabolism, Ketoglutaric Acids metabolism, Mitochondria metabolism
Published
1964

20. [Transport of pyruvate in rat liver mitochondria].

Author

Francavilla A, Meduri B, Paradies G, and Papa S

Subjects
Animals, Anti-Bacterial Agents pharmacology, Citrates pharmacology, Hydrogen-Ion Concentration, In Vitro Techniques, Ketoglutaric Acids pharmacology, Malates pharmacology, Malonates pharmacology, Organomercury Compounds pharmacology, Rats, Valinomycin pharmacology, Mitochondria, Liver metabolism, Pyruvates metabolism
Published
1971

32. [Nature of the citrate transport reaction in rat liver mitochondria].

Author

Lofrumento NE, Paradies G, Kanduc D, and Papa S

Subjects
Animals, Anti-Bacterial Agents pharmacology, Hydrogen-Ion Concentration, In Vitro Techniques, Rats, Valinomycin pharmacology, Citrates metabolism, Malates metabolism, Mitochondria, Liver metabolism
Published
1971

34. [Action of drugs uncoupling oxidative phosphorylation on anion transport in mitochondria].

Author

Zanghi MA, Papa S, Paradies G, and Quagliariello E

Subjects
Anti-Bacterial Agents pharmacology, Coumarins pharmacology, Dicumarol pharmacology, In Vitro Techniques, Mercury pharmacology, Mitochondria, Liver drug effects, Organometallic Compounds pharmacology, Oxidative Phosphorylation, Valine pharmacology, Citrates metabolism, Malates metabolism, Mitochondria, Liver metabolism, Phosphates metabolism
Published
1970

44. [Enzymatic localization in the sub-mitochondrial fractions of pigeon liver. I. Study of glutamic dehydrogenase, isocitrate dehydrogenase (NADP), the enzymes of fatty acid synthesis and phosphopyruvic carboxylase].

Author

Landriscina C, Papa S, Lofrumento NE, Coratelli P, and Quagliariello E

Subjects
Animals, Columbidae, Fatty Acids metabolism, Mitochondria, Liver metabolism, Carboxy-Lyases analysis, Glutamate Dehydrogenase analysis, Isocitrate Dehydrogenase analysis, Liver enzymology, Mitochondria, Liver enzymology, Oxidoreductases analysis
Published
1968

46. [Energy transfer and dissipation in submitochondrial particles].

Author

Simone S, Lorusso M, Guerrieri F, Papa S, and Quagliariello E

Subjects
Acetates pharmacology, Animals, Cattle, In Vitro Techniques, Nitrates pharmacology, Potassium pharmacology, Potassium Chloride pharmacology, Potassium Iodide pharmacology, Adenosine Triphosphatases metabolism, Energy Transfer, Mitochondria metabolism, Myocardium metabolism, Oxidative Phosphorylation, Oxidoreductases metabolism, Oxygen Consumption
Published
1971

47. [Effects of carcinogenic ortho-aminophenols on mitochondrial processes].

Author

Alifano A, Papa S, Gorrod JW, and Quagliariello E

Subjects
Aniline Compounds pharmacology, Biphenyl Compounds pharmacology, Naphthalenes pharmacology, ortho-Aminobenzoates pharmacology, Amines pharmacology, Carcinogens pharmacology, Mitochondria, Liver metabolism, Phenols pharmacology
Published
1965

48. [Metabolism of succinic acid in rat brain mitochondria].

Author

Papa S, Lofrumento NE, De Gregorio G, Paradies G, and Quagliariello E

Subjects
Adenosine Triphosphate pharmacology, Animals, Aspartic Acid biosynthesis, In Vitro Techniques, Oligomycins pharmacology, Oxaloacetates biosynthesis, Oxygen Consumption, Pyruvates biosynthesis, Rats, Spectrophotometry, Succinate Dehydrogenase metabolism, Brain metabolism, Mitochondria metabolism, Succinates metabolism
Published
1967

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