1. Association between spindle assembly checkpoint expression and maternal age in human oocytes.
- Author
-
Steuerwald N, Cohen J, Herrera RJ, Sandalinas M, and Brenner CA
- Subjects
- Adult, Cell Cycle Proteins, Female, Humans, Mad2 Proteins, Oocytes metabolism, Protein Serine-Threonine Kinases, Repressor Proteins, Calcium-Binding Proteins genetics, Maternal Age, Oocytes physiology, Protein Kinases genetics, Signal Transduction physiology, Spindle Apparatus physiology
- Abstract
The spindle assembly checkpoint modulates the timing of anaphase initiation in response to the improper alignment of chromosomes at the metaphase plate. If defects are detected, a signal is transduced to halt further progression of the cell cycle until correct bipolar attachment to the spindle is achieved. The mitotic arrest deficient (MAD2) and budding uninhibited by benomyl (BUB1) genes encode conserved kinetochore-associated proteins believed to be components of the checkpoint regulatory pathway. A failure in this surveillance system could lead to genomic instability that may underlie the increased incidence of aneuploidy in the gametes of older women. To explore this possibility, the concentrations of these transcripts in human oocytes at various stages of maturation were determined by real-time rapid cycle fluorescent reverse transcription-polymerase chain reaction (RT-PCR). The results obtained following quantitative analysis suggest that these messages degrade as oocytes age. Potentially, this may impair checkpoint function in older oocytes and may be a contributing factor in age-related aneuploidy.
- Published
- 2001
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