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To gain insights into the regulatory networks related to anthocyanin biosynthesis and identify key regulatory genes, we performed an integrated analysis of the transcriptome and metabolome in sprouts germinated from three colored potato cultivars: light-red Hongyoung, dark-purple Jayoung, and white Atlantic. We investigated transcriptional and metabolic changes using statistical analyses and gene-metabolite correlation networks. Transcript and metabolite profiles were generated through high-throughput RNA-sequencing data analysis and ultraperformance liquid chromatography quadrupole time-of-flight tandem mass spectrometry, respectively. The identification and quantification of changes in anthocyanin were performed using molecular formula-based mass accuracy and specific features of their MS2 spectra. Correlation tests of anthocyanin contents and transcriptional changes showed 823 strong correlations (correlation coefficient, R2>0.9) between 22 compounds and 119 transcripts categorized into flavonoid metabolism, hormones, transcriptional regulation, and signaling. The connection network of anthocyanins and genes showed a regulatory system involved in the pigmentation of light-red Hongyoung and dark-purple Jayoung potatoes, suggesting that this systemic approach is powerful for investigations into novel genes that are potential targets for the breeding of new valuable potato cultivars. [ABSTRACT FROM AUTHOR]

The article discusses a study which examined the cloning of a third β-glucoside operon from Pectobacterium carotovorum subspecies carotovorum LY34. The operon contains bglE, bglF, bglI and bglA genes. The amino acid sequence of bglA gene has similarity to various 6-phospho-β-glucosidases and to a member of glycosyl hydrolase family 1. The four genes were organized in a putative operaton that differed from the operons previously isolated from LY34.

Presents an analysis of bgl operon structure and characterization of β-glucosidase from Pectobacterium carotovorum subsp. carotovorum LY34. Isolation of a putative bgl operon of Pectobacterium carotovorum subsp. carotovorum LY34; Sequence analysis of the 5,557 bp cloned DNA fragment; Findings that two glutamate residues are essential for enzyme activity.

Discusses the endophytic colonization of balloon flower by the antifungal strain Bacillus sp. CY22. Evaluation of Bacillus sp. CY22R3 in external and internal root tissue; Antifungal substance produced by Bacillus sp. CY22; Biocontrol potential of Bacillus sp. CY22.