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The QuickTox Kit for QuickScan Aflatoxin FREE uses competitive lateral flow technology and a reader based system for quantitative determination of total aflatoxins in varied matrixes. Aqueous based extraction protocols are used for corn and wheat, reducing use of solvents. Fifty percent ethanol (Reagent Alcohol) extraction is used for oats, sorghum, and barley. Eighty percent ethanol (Reagent Alcohol) extraction is used for whole peanut, peanut seed, and peanut hull samples. Matrix specific assay procedures and calibration curves are used to enable analyses across multiple sample types. The performance of this assay was examined using naturally contaminated aflatoxin corn samples and spiked samples of barley, oats, sorghum, wheat, whole peanut, peanut seed, and peanut hull samples. All data were judged against previously established acceptance criteria. Performance was evaluated in linearity, selectivity, matrix, lot consistency, and robustness experiments in the sponsor's laboratory. Results produced in all studies except robustness were within acceptable ranges. Out of range robustness study results reflected simultaneous deviation in sample volume and assay development time compared to the standard assay procedures. Aflatoxin B1, B2, and G1 were detected with approximately equal sensitivity; sensitivity for G2 was 64% that of B1. The presence of other common mycotoxins did not interfere with the assay. Matrix studies in an independent laboratory examined corn and barley to challenge both aqueous and ethanol based extraction procedures. All data points in these studies fell within the ranges defined in the acceptance criteria. The assay exhibited a linear dose response over the range tested, 0-100 ppb, with R2 values exceeding 0.93 and RSDr values for results ranging from 2.27 to 23.84%. [ABSTRACT FROM AUTHOR]

Quantitative, reader-based lateral flow technology is utilized for determination of ochratoxin contamination levels in wheat by the QuickTox Kit for QuickScan Ochratoxin-A. Naturally contaminated wheat samples were used to challenge the assay in the range of 0-100 ppb in linearity, selectivity, robustness, and stability, and in internal and external matrix studies. Performance was judged against criteria established by the AOAC Research Institute Performance Tested MethodSM program prior to beginning the validation studies. All data produced during this work conformed to the acceptance criteria. Linear dose responses with R² exceeding 0.97 and RSDr values between 6.22 and 17.10% for positive samples were observed in linearity and internal and external matrix studies. Ochratoxin A (OTA) and ochratoxin B (OTB) were detected by the assay. Assay sensitivity towards OTB was approximately 50% relative to OTA detection. Other common mycotoxins did not affect assay results. Variations in assay timing, temperature, and sample volume encompassed in the robustness study did not yield results outside the acceptable range. Determination of ochratoxin in wheat is facilitated using the QuickTox Kit for QuickScan Ochratoxin-A kit. [ABSTRACT FROM AUTHOR]

A letter to the editor is presented referring to importance of interprofessional education (IPE) in outpatient care and development of interprofessional relations.