1. Lung-Derived Exosomal miR-483-3p Regulates the Innate Immune Response to Influenza Virus Infection.
- Author
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Tadashi Maemura, Satoshi Fukuyama, Yukihiko Sugita, Lopes, Tiago J. S., Tomomi Nakao, Takeshi Noda, Yoshihiro Kawaoka, Maemura, Tadashi, Fukuyama, Satoshi, Sugita, Yukihiko, Nakao, Tomomi, Noda, Takeshi, and Kawaoka, Yoshihiro
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MICRORNA , *INFLUENZA viruses , *BRONCHOALVEOLAR lavage , *CYTOKINES , *INTERFERONS - Abstract
Exosomes regulate cell-cell communication by transferring functional proteins and RNAs between cells. Here, to clarify the function of exosomes during influenza virus infection, we characterized lung-derived exosomal microRNAs (miRNAs). Among the detected miRNAs, miR-483-3p was present at high levels in bronchoalveolar lavage fluid (BALF) exosomes during infection of mice with various strains of influenza virus, and miR-483-3p transfection potentiated gene expression of type I interferon and proinflammatory cytokine upon viral infection of MLE-12 cells. RNF5, a regulator of the RIG-I signaling pathway, was identified as a target gene of miR-483-3p. Moreover, we found that CD81, another miR-483-3p target, functions as a negative regulator of RIG-I signaling in MLE-12 cells. Taken together, this study indicates that BALF exosomal miRNAs may mediate the antiviral and inflammatory response to influenza virus infection. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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