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Many species of the brachyuran crabs in the family Calappidae, commonly known as 'box crabs' or 'shame-faced crabs,' look alike and are difficult to distinguish at various developmental stages based on morphology alone. Some crabs recently collected as a part of the South East Atlantic Monitoring and Assessment Program (SEAMAP) groundfish surveys were identified as likely members of Calappidae, but could not be determined to species due to their unusual color pattern and size. The use of molecular data in combination with morphometrics suggests that the crabs in question belonged to Calappa flammea (Herbst, 1794). The specimens represent an undescribed unique color morph that deviates from their previous description and should be considered for future identifications. Several ecological factors can cause such color variations in crabs, but further investigation is needed to understand the drivers of the described phenotypic variance in C. flammea. [ABSTRACT FROM AUTHOR]

The cnidarian Nematostella vectensis has become an established lab model, providing unique opportunities for venom evolution research. The Nematostella venom system is multimodal: involving both nematocytes and ectodermal gland cells, which produce a toxin mixture whose composition changes throughout the life cycle. Additionally, their modes of interaction with predators and prey vary between eggs, larvae, and adults, which is likely shaped by the dynamics of the venom system. Nv1 is a major component of adult venom, with activity against arthropods (through specific inhibition of sodium channel inactivation) and fish. Nv1 is encoded by a cluster of at least 12 nearly identical genes that were proposed to be undergoing concerted evolution. Surprisingly, we found that Nematostella venom includes several Nv1 paralogs escaping a pattern of general concerted evolution, despite belonging to the Nv1-like family. Here, we show two of these new toxins, Nv4 and Nv5, are lethal for zebrafish larvae but harmless to arthropods, unlike Nv1. Furthermore, unlike Nv1, the newly identified toxins are expressed in early life stages. Using transgenesis and immunostaining, we demonstrate that Nv4 and Nv5 are localized to ectodermal gland cells in larvae. The evolution of Nv4 and Nv5 can be described either as neofunctionalization or as subfunctionalization. Additionally, the Nv1-like family includes several pseudogenes being an example of nonfunctionalization and venom evolution through birth-and-death mechanism. Our findings reveal the evolutionary history for a toxin radiation and point toward the ecological function of the novel toxins constituting a complex cnidarian venom. [ABSTRACT FROM AUTHOR]

Cubozoans (box jellyfish) have a reputation as the most venomous animals on the planet. Herein, we provide a review of cubozoan prey capture and digestion informed by the scientific literature. Like all cnidarians, box jellyfish envenomation originates from structures secreted within nematocyte post-Golgi vesicles called nematocysts. When tentacles come in contact with prey or would-be predators, a cocktail of toxins is rapidly deployed from nematocysts via a long spiny tubule that serves to immobilize the target organism. The implication has long been that toxin peptides and proteins making up the venom within the nematocyst capsule are secreted directly by nematocytes during nematogenesis. However, our combined molecular and morphological analysis of the venomous box jellyfish Alatina alata suggests that gland cells with possible dual roles in secreting toxins and toxic-like enzymes are found in the gastric cirri. These putative gland cell assemblages might be functionally important internally (digestion of prey) as well as externally (envenomation) in cubozoans. Despite the absence of nematocysts in the gastric cirri of mature A. alata medusae, this area of the digestive system appears to be the region of the body where venom-implicated gene products are found in highest abundance, challenging the idea that in cnidarians venom is synthesized exclusively in, or nearby, nematocysts. In an effort to uncover evidence for a central area enriched in gland cells associated with the gastric cirri we provide a comparative description of the morphology of the digestive structures of A. alata and Carybdea box jellyfish species. Finally, we conduct a multi-faceted analysis of the gene ontology terms associated with venom-implicated genes expressed in the tentacle/pedalium and gastric cirri, with a particular emphasis on zinc metalloprotease homologs and genes encoding other bioactive proteins that are abundant in the A. alata transcriptome. [ABSTRACT FROM AUTHOR]

Cnidarians represent one of the few groups of venomous animals that lack a centralized venom transmission system. Instead, they are equipped with stinging capsules collectively known as nematocysts. Nematocysts vary in abundance and type across different tissues; however, the venom composition in most species remains unknown. Depending on the tissue type, the venom composition in sea anemones may be vital for predation, defense, or digestion. Using a tissue-specific RNA-seq approach, we characterize the venom assemblage in the tentacles, mesenterial filaments, and column for three species of sea anemone (Anemonia sulcata, Heteractis crispa, and Megalactis griffithsi). These taxa vary with regard to inferred venom potency, symbiont abundance, and nematocyst diversity. We show that there is significant variation in abundance of toxin-like genes across tissues and species. Although the cumulative toxin abundance for the column was consistently the lowest, contributions to the overall toxin assemblage varied considerably among tissues for different toxin types. Our gene ontology (GO) analyses also show sharp contrasts between conserved GO groups emerging from whole transcriptome analysis and tissue-specific expression among GO groups in our differential expression analysis. This study provides a framework for future characterization of tissue-specific venomand other functionally important genes in this lineage of simple bodied animals. [ABSTRACT FROM AUTHOR]