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Aims: Crown gall, a phytobacteriosis characterized by the formation of tumours on plant roots was observed in recently planted vineyards of the Meknes region (Morocco). The objective of this research was to analyse the diversity of pathogenic agrobacteria isolated from grapevine in Morocco. Methods and Results: Eighty‐two isolates from 11 affected vineyards were characterized by recA sequencing and were found to belong to Agrobacterium tumefaciens genomospecies G1, G4 or G7, Rhizobium rhizogenes, and to Allorhizobium vitis. Only the All. vitis isolates appeared to be pathogenic on tomato and multilocus sequence analysis phylogenetic analyses revealed a weak genetic diversity, with the definition of only four genomic groups. Definition of the All. vitis genomic groups correlated with specific pathogenic traits: indeed, genomic groups differed with respect to the severity of hypersensitive response symptoms on tobacco leaves, the intensity of necrotic response on grapevine explants and opine profiles. Both vitopine and octopine were detected by UHPLC in tumours induced by isolates of three genomic groups, an opine signature scarcely ever reported. Conclusions: Allorhizobium vitis is the only causative agent of crown gall on grape in Morocco, pathogenic isolates can be separated into four genomic groups. Significance and Impact of the Study: This study of recently crown‐gall‐infested vineyards demonstrated that All. vitis is the only causative agent and revealed the presence of nonpathogenic Agrobacterium strain within tumours. Moreover, as the genetic diversity of the All. vitis isolates is relatively narrow, this study lays the basis for further analyses on the evolution of the disease, on the dissemination of the pTi and more globally on the fate of the different genomic groups in this newly colonized environment. [ABSTRACT FROM AUTHOR]

Leifsonia xyli subsp . xyli (Lxx), causal organism of ratoon stunt ( RSD), does not produce any reliable internal or external symptoms on sugarcane. Its detection on a large scale is solely based on microscopic and serological methods. These methods require well-equipped laboratories, are time consuming and are not feasible for near-field detection of Lxx. In this study, we developed a loop-mediated isothermal amplification ( LAMP) assay for rapid and sensitive detection of Lxx without the use of sophisticated equipment. To the best of our knowledge, this is the first report on the detection of Lxx in 30 min via an isothermal amplification method at 65°C. A transposase gene, ISLxx5, was used to design a set of six primers specifically targeting eight genomic sequences. The xylem sap was used as template, thus circumventing the need to isolate pure genomic DNA. The positive reactions were visually detected through a colour change of hydroxynaphthol blue ( HNB) from violet to light blue, thus, eliminating the need for gel electrophoresis. The LAMP method was 10 times more sensitive than serological detection and as sensitive as immunofluorescence microscopy ( IFM). The simplicity and sensitivity of the ISLxx5 LAMP assay makes it suitable for near-field diagnosis of RSD. Significance and Impact of the Study Detection of Leifsonia xyli subsp. xyli ( Lxx) on a large scale is based on serological assays such as evaporative-binding enzyme-linked immunoassay ( EB- EIA). These methods are time consuming and require well-equipped laboratories. This study presents the development of a loop-mediated isothermal amplification ( LAMP) assay which allows detection of Lxx in 30 min at 65°C, using xylem sap as the template. The assay requires minimal laboratory equipment and could be used at near farm conditions, thus saving time and money required to transfer samples from remote areas to diagnostic laboratories. The LAMP method shows potential as an alternative detection method for RSD. [ABSTRACT FROM AUTHOR]

With the advances in cloud computing and huge RAMs provided by 64-bit architectures, it is possible to tackle large problems using memory-based solutions. Construction of term-based, partitioned, parallel inverted indexes is a communication intensive task and suitable for memory-based modeling. In this paper, we provide an efficient parallel framework for in-memory construction of term-based partitioned, inverted indexes. We show that, by utilizing an efficient bucketing scheme, we can eliminate the need for the generation of a global vocabulary. We propose and investigate assignment schemes that can reduce the communication overheads while minimizing the storage and final query processing imbalance. We also present a study on how communication among processors should be carried out with limited communication memory in order to reduce the total inversion time. We present several different communication-memory organizations and discuss their advantages and shortcomings. The conducted experiments indicate promising results. [ABSTRACT FROM PUBLISHER]

RNA editing sites were systematically examined for the transcripts of 74 known protein-coding genes in the chloroplasts of Phalaenopsis aphrodite. A total of 44 editing sites were identified in 24 transcripts, the highest reported in seed plants. In addition, 21 editing sites are unique to the Phalaenopsis orchid as compared with other seed plants. All editing is C-to-U conversion, and 42 editing sites bring about the changes in amino acids. One of the remaining two editing sites occurs in the transcripts of the ndhB pseudogene, and another in the 5′-untranslated region of psbH transcripts. [ABSTRACT FROM AUTHOR]

The crdS gene of Agrobacterium sp. strain ATCC31749 encodes the curdlan synthase (CrdS) protein based on the homology of the derived CrdS protein sequence with those of β-glycosyl transferases with repetitive action patterns (Stasinopoulos et al. [1999] Glycobiology, 9, 31-41). Here we show that chemical (NTG) mutagenesis of crdS abolishes curdlan production and the induced mutations can be complemented by a cloned crdS amplicon, thus providing genetic confirmation that crdS is essential for curdlan production. When expressed in the native Agrobacterium or in Escherichia coli, the largely hydrophobic CrdS protein exhibited an Mr of ∼60 kDa (compared with the predicted mass of 73,121 Da) and was located in the inner membrane of both bacteria. By analyzing reciprocal fusions between crdS and the reporter genes, lacZ and phoA, and assessing the sensitivity of CrdS in spheroplasts to proteinase K, CrdS was shown to be an integral membrane protein with seven transmembrane helices and an Nout-Cin disposition. A central large and relatively hydrophilic cytoplasmic region carries the substrate-binding and catalytic D,D,D35QxxRW motif. The amino acid sequence of this domain of CrdS was threaded onto the 3D structure of the comparable domain of the SpsA protein, a member of the family GT-2 glycosyl transferases, and enabled the identification of corresponding amino acids involved in binding UDP in CrdS. Analysis of Agrobacterium membrane preparations using blue native-PAGE provided preliminary evidence that CrdS occurs in multimeric protein complexes of ∼420 kDa and ∼500 kDa. [ABSTRACT FROM AUTHOR]