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This work aimed to develop an indirect enzyme-linked immunosorbent assay (i- ELISA), based on the structural protein VP2 of goose parvovirus ( GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i- ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization ( SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97·3%. This i- ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum. Significance and Impact of the Study In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2-based i- ELISA for the detection of GPV antibodies. This i- ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection. [ABSTRACT FROM AUTHOR]

We present a multiwavelength spectral and temporal analysis of the blazar OJ 287 during its recent activity between 2015 December and 2016 May, showing strong variability in the nearinfrared (NIR) to X-ray energies with detection at γ -ray energies as well. Most of the optical flux variations exhibit strong changes in polarization angle and degree. All the interband time lags are consistent with simultaneous emissions. Interestingly, on days with excellent data coverage in the NIR-UV bands, the spectral energy distributions (SEDs) show signatures of bumps in the visible-UV bands, never seen before in this source. The optical bump can be explained as accretion-disc emission associated with the primary black hole of mass ~1.8 × 1010 M☉ while the little bump feature in the optical-UV appears consistent with line emission. Further, the broad-band SEDs extracted during the first flare and during a quiescent period during this span show very different γ -ray spectra compared to previously observed flare or quiescent spectra. The probable thermal bump in the visible seems to have been clearly present since 2013 May, as found by examining all available NIR-optical observations, and favours the binary supermassive black hole model. The simultaneous multiwavelength variability and relatively weak γ -ray emission that shows a shift in the SED peak is consistent with γ -ray emission originating from inverse Compton scattering of photons from the line emission that apparently contributes to the little blue bump. [ABSTRACT FROM AUTHOR]

We analysed the multiband optical behaviour of the BL Lacertae object, S5 0716+714, during its outburst state from 2014 November to 2015 March. We took data on 23 nights at three observatories, one in India and two in Bulgaria, making quasi-simultaneous observations in B, V, R, and I bands. We measured multiband optical fluxes, colour, and spectral variations for this blazar on intraday and short time-scales. The source was in a flaring state during the period analysed and displayed intense variability in all wavelengths. R-band magnitude of 11.6 was attained by the target on 2015 January 18, which is the brightest value ever recorded for S5 0716+714. The discrete correlation function method yielded good correlation between the bands with no measurable time lags, implying that radiation in these bands originate from the same region and by the same mechanism. We also used the structure function technique to look for characteristic time-scales in the light curves. During the times of rapid variability, no evidence for the source to display spectral changes with magnitude was found on either of the time-scales. The amplitude of variations tends to increase with increasing frequency with a maximum of ~22 per cent seen during flaring states in B band. A mild trend of larger variability amplitude as the source brightens was also found. We found the duty cycle of our source during the analysed period to be ~90 per cent. We also investigated the optical spectral energy distribution of S5 0716+714 using B, V, R, and I data points for 21 nights. We briefly discuss physical mechanisms most likely responsible for its flux and spectral variations. [ABSTRACT FROM AUTHOR]