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Several telescopes include large deformable mirrors (DM) located directly inside the telescope. These adaptive telescopes trigger new constraints for the calibration of the adaptive optics (AO) systems as they usually offer no access to an artificial calibration source for the interaction matrix measurement. Moreover, the optical propagation between the DM and the wavefront sensor (WFS) may evolve during the operation, resulting in misregistrations that highly affect the AO performance and thus the scientific observation. They have to be measured and compensated, for instance by updating the calibration. A new strategy consists of estimating the misregistrations and injecting them into synthetic models to generate noise-free interaction matrices. This pseudo-synthetic approach is the baseline for the adaptive optics facility working with a Shack–Hartmann WFS and seems particularly suited for the future Extremely Large Telescope as the calibration will have to be regularly updated, for a large numbers of actuators. In this paper, the feasibility of a pseudo-synthetic calibration with pyramid WFS at the Large Binocular Telescope (LBT) is investigated. A synthetic model of the LBT AO systems is developed, and the procedure to adjust the misregistrations parameters is introduced, extracting them from an experimental interaction matrix. We successfully tested an interaction matrix generated from the model on the real system in high-order AO mode. We recorded a slightly better performance with respect to the experimental one. This work demonstrates that a high-accuracy calibration can be obtained using the pseudo-synthetic approach with pyramid WFS. [ABSTRACT FROM AUTHOR]

We show numerical simulations with monochromatic light in the visible for the LBTI Fizeau imager, including opto-dynamical aberrations due here to adaptive optics (AO) errors and to differential piston fluctuations, while other errors have been neglected. The achievable Strehl by the LBTI using two AO is close to the Strehl provided by a single standalone AO system, as long as other differential wavefront errors are mitigated. The LBTI Fizeau imager is primarily limited by the AO performance and by the differential piston/tip-tilt errors. Snapshots retain high-angular resolution and high-contrast imaging information by freezing the fringes against piston errors. Several merit functions have been critically evaluated in order to characterize point spread functions and the modulation transfer functions for highcontrast imaging applications. The LBTI Fizeau mode can provide an image quality suitable for standard science cases (i.e. a Strehl above 70 per cent) by performing both at a time: an AO correction better than ≈λ/18 RMS for both short and long exposures, and a piston correction better than ≈λ/8 RMS for long exposures or simply below the coherence length for short exposures. Such results, which can be applied to any observing wavelength, suggest that AO and piston control at the LBTI would already improve the contrast at near- and mid-infrared wavelengths. Therefore, the LBTI Fizeau imager can be used for high-contrast imaging, providing a high-Strehl regime (by both AO systems), a cophasing mode (by a fringe tracker) and a burst mode (by a fast camera) to record fringed speckles in short exposures. [ABSTRACT FROM AUTHOR]

We show by numerical simulations a fundamental gain in contrast when combining coherently monochromatic light from two adaptive optics (AO) telescopes instead of using a single stand-alone AO telescope, assuming efficient control and acquisition systems at high speed. A contrast gain map is defined as the normalized point spread functions (PSFs) ratio of a single Large Binocular Telescope (LBT) aperture over the dual Large Binocular Telescope Interferometer (LBTI) aperture in Fizeau mode. The global gain averaged across the AO-corrected field of view is improved by a factor of 2 in contrast in long exposures and by a factor of 10 in contrast in short exposures (i.e. in exposures, respectively, longer or shorter than the coherence time). The fringed speckle halo in short exposures contains not only highangular resolution information, as stated by speckle imaging and speckle interferometry, but also high-contrast imaging information. A high-gain zone is further produced in the valleys of the PSF formed by the dark Airy rings and/or the dark fringes. Earth rotation allows us to exploit various areas in the contrast gain map. A huge-contrast gain in narrow zones can be achieved when both a dark fringe and a dark ring overlap on to an exoplanet. Compared to a single 8-m LBT aperture, the 23-m LBTI Fizeau imager can provide a gain in sensitivity (by a factor of 4), a gain in angular resolution (by a factor of 3) and, as well, a gain in raw contrast (by a factor of 2-1000 varying over the AO-corrected field of view). [ABSTRACT FROM AUTHOR]

We derive new empirical calibrations for strong-line diagnostics of gas-phase metallicity in local star-forming galaxies by uniformly applying the Te method over the full metallicity range probed by the Sloan Digital Sky Survey (SDSS). To measure electron temperatures at high metallicity, where the auroral lines needed are not detected in single galaxies, we stacked spectra of more than 110 000 galaxies from the SDSS in bins of log[O II]/Hß and log[O III]/Hß. This stacking scheme does not assume any dependence of metallicity on mass or star formation rate, but only that galaxies with the same line ratios have the same oxygen abundance. We provide calibrations which span more than 1 dex in metallicity and are entirely defined on a consistent absolute Te metallicity scale for galaxies. We apply our calibrations to the SDSS sample and find that they provide consistent metallicity estimates to within 0.05 dex. [ABSTRACT FROM AUTHOR]

Background: Data on milk and other dairy products intake with health outcomes are inconsistent. We investigated the association of milk and total dairy consumption with the risk of all-cause and cause-specific mortality in a Mediterranean population. Methods: We performed a longitudinal analysis on 22,889 men and women (mean age 55.4[11.7 y; 52.3% women), from the Moli-sani Study cohort (enrollment 2005-2010) followed-up for a median of 11 years. Dietary data were collected through a 188-item food frequency questionnaire. Total dairy consumption (g/d) was calculated as the sum of milk (whole and partially-skimmed, g/d), yogurt (whole and partially skimmed, g/d) and cheese (fresh and hard, g/d). Hazard ratio (HRs) with 95% confidence interval (CI) were calculated by multivariable Cox regression, modelled by spline curves. Results: In multivariable-adjusted analysis controlled for sociodemographic, clinical and dietary factors, cheese, yogurt or whole milk intake were not associated with either all-cause or causespecific mortality (p for overall association >0.38), as well as was total diary intake (p > 0.18). On the contrary, non-linear Jshaped curves were observed for the association of partially skimmed milk intake with all-cause and cancer mortality (p value for total and non-linear association=0.0072 and 0.035, and 0.097 and 0.049, respectively; magnitude of the relative reduction =16% and 18% at nadir of 128 and 114 g/d, respectively). A linear association between partially skimmed milk intake and CVD mortality was observed (p for association =0.047; p for non-linearity= 0.41), with HR= 0.82 (95%CI: 0.68-0.98) at 125 g/d and a window of statistically significant protection ranging from 100 to 250 g/d. Conclusions: In a large Mediterranean population of adults, consumption of 1 standard cup of partially skimmed milk was associated with lower all-cause, cancer and CVD mortality. Other dairy sources were not associated with mortality. Key messages: • In a large Mediterranean population, total dairy intake did not predict mortality. • Daily consumption of 1 standard cup of partially skimmed milk lowers the risk of all-cause, cardiovascular and cancer mortality. [ABSTRACT FROM AUTHOR]

Background: The Nutri-Score front-of-pack label rates foods according to their nutritional quality while the NOVA classification is focused on degree of processing; both systems separately predicted mortality in epidemiological contexts. We evaluated whether ultra-processed food (UPF) modifies the association of the Nutri-Score with mortality. Methods: Longitudinal analysis on 22,549 Italian men and women (mean age 54±12 y) from the Moli-sani Study (2005-2010) followed for 11.2 y. Food intake was assessed by a food frequency questionnaire. The Food Standards Agency nutrient profiling system (FSAm-NPS) score, used to derive the Nutri-Score, was calculated for each food based on its amount of energy, saturated fat, sugar, sodium, fibre, protein, fruits, vegetables, legumes and nuts per 100 g of product. The FSAm-NPS dietary index was calculated for each participant as an energy weighted mean of the FSAm-NPS score of all foods consumed, and increased values indicate lower nutritional quality. UPF intake was the proportion (%) of UPF in the total weight of food consumed (g/d) and categorized as low/high (below/above the population median, respectively). Results: In a multivariable analysis, 1-SD increase in the FSAm-NPS index led to increased risk of all-cause (HR = 1.07; 95%CI 1.02-1.13) and CVD mortality (HR = 1.08; 1.00-1.18). UPF intake was an effect modifier since the association of FSAm-NPS index with mortality was confined to individuals with high UPF intake (HR = 1.14; 1.05-1.25) but not in those with low UPF (HR = 1.00; 0.93-1.07; p for interaction=0.034). Similar findings were reported for CVD mortality (HR = 1.14;0.99-1.31 and HR = 1.01;0.90-1.13 for high and low UPF consumers, respectively; p for interaction=0.18). Conclusions: Higher mortality risk associated with a NutriScore revealing nutrient-poor food intake is limited to individuals also reporting high UPF intake. Food labelling systems should account for food processing in addition to nutritional content. Key messages: • Ultra-processed food modifies the association between the 5-color Nutri-Score front-of-pack label with all-cause mortality in an Italian general population. • Food labelling systems, that are conceived to help consumers make healthier food choices, should also account for food processing in addition to nutritional content. [ABSTRACT FROM AUTHOR]

Objectives: Cone beam CT (CBCT) produces undistorted three-dimensional (3D) images of the maxillofacial region with a radiation dosage lower than conventional CT. The periapical index score (PAI) is commonly used to follow up the lesions in the bone using periapical radiographs. Recently, a new PAI based on CBCT was introduced (CBCT-PAI). The aim of this technical report is to present a modified reproducible method to assess the CBCT-PAI. Methods: CBCT was used to evaluate a periapical bone lesion observed in the area of tooth number 13 before treatment and 2 years after treatment. The modified CBCT-PAI was applied to both the examinations to measure the lesion. The dimensional analysis of the lesion was performed in each plane, assessing three fixed and reproducible dimensions: mesiodistal (M-D), buccolingual (B-L) and coronoapical (C-A). The images were evaluated by three mutually independent examiners. Data were collected and reported in a chart. The results were compared with each other and with the PAI score from the periapical radiographs. Results: The three observers reported the same measurements of the lesion for each plane. The CBCT-PAI follow-up showed a reduction of the size of the lesion (5D vs 4D) but also an increase in the erosion of the buccal cortical plate. The comparison of CBCT-PAI with classic PAI showed the first method to be more precise. Conclusions: This technical report shows how the CBCT-PAI can be applied to the CBCT exam of a periapical lesion in a reproducible way. [ABSTRACT FROM AUTHOR]

Objectives Mycoplasma pneumoniae respiratory infection is a common cause of acute respiratory infection in children and adults. We evaluated the efficacy of increasing dosages of clarithromycin for the optimized therapy of M. pneumoniae respiratory infection in a mouse model. Methods BALB/c mice were intranasally inoculated once with M. pneumoniae or SP4 broth (control). Groups of mice were treated with increasing dosages of clarithromycin (10, 25 or 75 mg/kg/day) or placebo subcutaneously daily. Groups of mice were evaluated after 1, 2, 3, 6 and 12 days of therapy. Outcome variables included quantitative M. pneumoniae culture, histopathological score of the lungs, bronchoalveolar lavage (BAL) cytokine/chemokine/growth factor concentrations and plethysmography after aerosolized methacholine to assess airway hyperresponsiveness. Results Elevated dosages of clarithromycin resulted in greater antimicrobial efficacy with significantly reduced M. pneumoniae quantitative cultures (P < 0.05), as well as greater improvement in markers of disease severity with significantly reduced lung histopathology scores, BAL cytokine concentrations and airway hyperresponsiveness (P < 0.05). Conclusions Escalated dosing of clarithromycin resulted in significantly greater therapeutic efficacy in the treatment of experimental M. pneumoniae respiratory infection. [ABSTRACT FROM PUBLISHER]

Objectives Mycoplasma pneumoniae is a frequent cause of human lower respiratory tract infections (LRTIs) for which macrolides are the treatment of choice. The aim of this study was to determine the rate of macrolide resistance and to subtype M. pneumoniae strains in Italy. Patients and methods During an outbreak of M. pneumoniae infections in southern Italy in 2010, 48 clinical specimens from 43 paediatric patients hospitalized for LRTIs were analysed for macrolide resistance. The mutations associated with resistance (A2063G and A2064G) and M. pneumoniae subtypes were detected by sequencing the targeted domain V region of the 23S rRNA gene and a region in the MPN528a gene, respectively. Results Macrolide resistance genotypes were detected in 11 (26%) of the 43 M. pneumoniae-positive children. The A2063G mutation was identified in seven patients and the A2064G mutation was identified in the remaining four. Upon admission, the isolates from three patients showed a susceptible genotype but subsequently acquired the A2063G mutation. Genotyping revealed M. pneumoniae subtype 1 in 33 of 40 sequenced strains and subtype 2 in the remaining 7. There was no association between macrolide resistance or susceptibility and the M. pneumoniae subtypes. Conclusions This is the first report of macrolide resistance among M. pneumoniae strains in Italy. Our findings indicate an unexpected high prevalence of macrolide resistance genotypes in children, and so macrolide resistance should be carefully considered in patients who do not respond appropriately to antibiotic treatment. The epidemiological monitoring of macrolide resistance has become necessary in Italy and in the rest of Europe. [ABSTRACT FROM PUBLISHER]

Background. Systemic steroids have been advocated in addition to antimicrobial therapy for severe Mycoplasma pneumoniae pneumonia. We evaluated the efficacy of clarithromycin, dexamethasone, and combination therapy for M. pneumoniae respiratory infection. Methods. Mice infected with M. pneumoniae were treated with clarithromycin, dexamethasone, combined clarithromycin/ dexamethasone, or placebo daily; mice were evaluated at baseline and after 1, 3, and 6 days of therapy. Outcome variables included M. pneumoniae culture, lung histopathologic score (HPS), and bronchoalveolar lavage cytokine, chemokine, and growth factor concentrations. Results. Clarithromycin monotherapy resulted in the greatest reductions in M. pneumoniae concentrations. After 3 days of treatment, combination therapy significantly reduced lung HPS compared with placebo, clarithromycin, and dexamethasone alone, whereas, after 6 days of therapy, clarithromycin alone and combination therapy significantly reduced lung HPS compared with placebo. Concentrations of interleukin (IL)-12 p40, RANTES, macrophage chemotactic protein-1, and cytokine-induced neutrophil chemoattractant were significantly lower in mice treated with clarithromycin alone and/or combination therapy compared with dexamethasone alone and/or placebo; combination therapy resulted in a significantly greater reduction than clarithromycin alone for IL-12 p40 and RANTES. Conclusions. Although monotherapy with clarithromycin had the greatest effect on reducing concentrations of M. pneumoniae, combination therapy had the greatest effect on decreasing levels of cytokines and chemokines as well as pulmonary histologic inflammation. [ABSTRACT FROM AUTHOR]

Gram-positive cocci are a major cause of nosocomial bacteraemias and are often resistant to most antibiotics. The emergence of enterococci with reduced susceptibility to vancomycin has created an urgent need for novel antibiotics to combat infections associated with these bacteria. In this study the in vitro activity of LY333328 (oritavancin), a semi-synthetic glycopeptide, was evaluated. LY333328 was effective against all strains of staphylococci, streptococci and enterococci tested. A combination of LY333328 and ciprofloxacin was additive for 50% of Enterococcus faecium strains resistant to both vancomycin and ciprofloxacin, and for 100% of vancomycin-susceptible and either ciprofloxacin-susceptible or -resistant strains. [ABSTRACT FROM AUTHOR]

Sulbactam is a new β-lactamase inhibitor with pharmacokinetic characteristics in humans similar to those of ampicillin. A total of 41 patients hospitalized in the Clinic of Infectious Diseases, University of Naples, for chronic liver diseases, were treated with sulbactam/ampicillin (ratio 1: 2) for urinary, respiratory, biliary tract or soft tissue infections. Sulbactam/ampicillin was administered im or iv at a dosage of 3–9 g/day depending on the site and severity of the infection. All the patients treated with sulbactam/ampicillin had clinical signs and symptoms of infection, and all the organisms isolated were sensitive to sulbactam/ampicillin (MIC less than 16 mg/l). For both Gram-positive and Gram-negative bacteria the sulbactam/ampicillin MICs were much lower than the ampicillin MICs. In agreement with the favourable in-vitro results, we observed good therapeutic efficacy. 85% of the patients recovered or improved within a few days of therapy, with no clinical relapses, and in 81% of the infections the responsible bacteria were completely eradicated. We observed a low number of side effects (3/41 oral candidosis; 3/41 pain at the im injection site) and no change in the blood chemistry tests. [ABSTRACT FROM PUBLISHER]

It is shown that, using a pulsed laser to generate a laser guide star (LGS), a frame transfer technique at the focal plane of an auxiliary telescope can be used to retrieve the bidimensional tilt of such an LGS. We discuss how this approach can be used to reduce from two to one the number of auxiliary telescopes in the NGS-based perspective technique proposed in the literature. A discussion of the requirements for the laser pulse format is given. [ABSTRACT FROM AUTHOR]

Objective: To investigate whether and how the severity of medial degeneration (MD) lesions varies along the circumference of the dilated intrapericardial aorta. Methods: Two groups of aortic wall specimens, respectively harvested 1 cm distal to the non-coronary (NC) sinus (right postero-lateral wall) and to the right coronary sinus (anterior wall) in 22 patients undergoing surgery for dilatation of the intrapericardial aorta associated with aortic valve disease, were separately sent for pathology, morphometry and ultrastructural examination. MD lesions found at histology were classified into three degrees of severity. MD mean degree and morphometric findings in postero-lateral (‘NC’) and anterior (‘coronary’) specimens were compared by paired t-test. Correlation between degree of aortic dilatation at echocardiography and severity of MD was assessed separately for each of the two groups of specimens. After the preliminary results of the morphological study, we decided to send the specimens for biochemical investigation of protein electrophoretic patterns. This was performed in the last seven patients of this series. Results: At histology, MD was found in all cases. A higher mean MD degree was found in the NC group (2.59±0.50 versus 1.59±0.67 in the coronary group; P<0.001). At morphometry, normal smooth muscle cells in the NC specimens were significantly reduced (P=0.012) and the length (P=0.011) and number (P=0.015) of elastic fibres reduced and increased, respectively. Correlation between aortic ratio and MD degree was significant in the NC specimens (P<0.001), not in the coronary ones (P=0.227). Quantitative differences between coronary and NC proteins from the same patient and between coronary proteins from different patients were found at electrophoresis. However, at this stage of the study, the sample was too small to allow for the identification of proteins involved in those differences. Conclusions: MD lesions in dilated intrapericardial aorta are more severe in the right postero-lateral wall area, likely due to haemodynamic stress asymmetry. [Copyright &y& Elsevier]