1. Engineering Trienoic Fatty Acids into Cottonseed Oil Improves Low-Temperature Seed Germination, Plant Photosynthesis and Cotton Fiber Quality.
- Author
-
Gao, Lihong, Chen, Wei, Xu, Xiaoyu, Zhang, Jing, Singh, Tanoj K, Liu, Shiming, Zhang, Dongmei, Tian, Lijun, White, Adam, Shrestha, Pushkar, Zhou, Xue-Rong, Llewellyn, Danny, Green, Allan, Singh, Surinder P, and Liu, Qing
- Subjects
COTTONSEED oil ,FATTY acids ,COTTON fibers ,COTTON quality ,TRANSGENE expression ,FATTY acid desaturase ,COTTON - Abstract
Alpha-linolenic acid (ALA, 18:3Δ
9,12,15 ) and γ-linolenic acid \ (GLA, 18:3Δ6,9,12 ) are important trienoic fatty acids, which are beneficial for human health in their own right, or as precursors for the biosynthesis of long-chain polyunsaturated fatty acids. ALA and GLA in seed oil are synthesized from linoleic acid (LA, 18:2Δ9,12 ) by the microsomal ω-3 fatty acid desaturase (FAD3) and Δ6 desaturase (D6D), respectively. Cotton (Gossypium hirsutum L.) seed oil composition was modified by transforming with an FAD3 gene from Brassica napus and a D6D gene from Echium plantagineum , resulting in approximately 30% ALA and 20% GLA, respectively. The total oil content in transgenic seeds remained unaltered relative to parental seeds. Despite the use of a seed-specific promoter for transgene expression, low levels of GLA and increased levels of ALA were found in non-seed cotton tissues. At low temperature, the germinating cottonseeds containing the linolenic acid isomers elongated faster than the untransformed controls. ALA-producing lines also showed higher photosynthetic rates at cooler temperature and better fiber quality compared to both untransformed controls and GLA-producing lines. The oxidative stability of the novel cottonseed oils was assessed, providing guidance for potential food, pharmaceutical and industrial applications of these oils. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF