1. Fasting Serum Lipid and Dehydroepiandrosterone Sulfate as Important Metabolites for Detecting Isolated Postchallenge Diabetes: Serum Metabolomics via Ultra-High-Performance LC-MS
- Author
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Yucun Niu, Mingxin Bi, Changhao Sun, Liyan Liu, Ying Li, Maoqing Wang, Lixin Na, and Xue Yang
- Subjects
Male ,medicine.medical_specialty ,Metabolite ,Linoleic acid ,Clinical Biochemistry ,chemistry.chemical_compound ,Dehydroepiandrosterone sulfate ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Diabetes mellitus ,Internal medicine ,medicine ,Metabolome ,Humans ,Metabolomics ,Chromatography, High Pressure Liquid ,Dehydroepiandrosterone Sulfate ,Biochemistry (medical) ,Area under the curve ,Fasting ,Middle Aged ,Lipid Metabolism ,medicine.disease ,Lipids ,Postprandial ,Endocrinology ,Diabetes Mellitus, Type 2 ,chemistry ,Female - Abstract
BACKGROUND Isolated postchallenge diabetes (IPD), a subtype of type 2 diabetes mellitus (T2DM) defined as 2-h postprandial plasma glucose ≥200 mg/dL (≥11.1 mmol/L) and fasting plasma glucose (FPG) METHODS We applied a nontargeted metabolomic approach using ultra-high-performance liquid chromatography–quadrupole TOF–mass spectrometry (UPLC-QTOF-MS) to analyze serum samples from 51 patients with IPD, 52 with newly diagnosed T2DM, and 49 healthy individuals. We processed metabolite profiles by multivariate analysis to identify potential metabolites, which were further confirmed by tandem MS (MS/MS). We also used GC-MS and ELISA methods to detect potentially important metabolites. A number of independent samples were selected to validate the identified candidates. RESULTS We selected 15 metabolites with a view to distinguishing patients with IPD, whereas 11 were identified with an authentic standard. The selected metabolites included linoleic acid, oleic acid, phospholipids, and dehydroepiandrosterone sulfate (DHEA-S). In IPD samples, significantly higher linoleic and oleic acid (P < 0.001) and lower DHEA-S (P < 0.001) concentrations were observed, compared with controls. The area under the curve from a combination of linoleic acid, oleic acid, and DHEA-S in the validation study was 0.849 for the IPD group. CONCLUSIONS The current study provides useful information to bridge the gaps in our understanding of the metabolic alterations associated with IPD and might facilitate the characterization of patients with IPD by the use of fasting serum.
- Published
- 2013