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Aims This study aimed to investigate the potential of Bacillus methylotrophicus as a probiotic. Methods and Results A Bacillus isolate designated strain C14 was isolated from Korean traditional fermented soybean paste (doenjang). The strain was identified, and its physiological and biochemical properties were characterized. The gastrointestinal tolerance and immunomodulatory function of strain C14 were also investigated. Strain C14 was identified as B. methylotrophicus by analysis of its biochemical properties using the API 50CHB system and by phylogenetic analysis of the 16S rDNA sequence. Strain C14 showed >80% and >75% of survival for artificial gastric juices (pH 2·5 and 1% pepsin) and 0·5% (w/v) bile salt, respectively. Heat-killed B. methylotrophicus C14 inhibited the adhesion of various pathogens and enhanced the adhesion of probiotic bacteria to Caco-2 cells. The heat-killed cells also induced high levels of immune cell proliferation compared with the control and stimulated interleukin-6 and tumour necrosis factor-α production in mouse macrophages. Conclusions Bacillus methylotrophicus C14 could be used as a probiotic. Significance and Impact of the Study Recently identified B. methylotrophicus is a new potential probiotic with high gastrointestinal tolerance.

We present photometric observations of four transits in the WASP-17 planetary system, obtained using telescope defocussing techniques and with scatters reaching 0.5 mmag per point. Our revised orbital period is 4.0 +/- 0.6 s longer than previous measurements, a difference of 6.6 sigma, and does not support the published detections of orbital eccentricity in this system. We model the light curves using the JKTEBOP code and calculate the physical properties of the system by recourse to five sets of theoretical stellar model predictions. The resulting planetary radius, Rb = 1.932 +/- 0.052 +/- 0.010 Rjup (statistical and systematic errors respectively), provides confirmation that WASP-17b is the largest planet currently known. All fourteen planets with radii measured to be greater than 1.6 Rjup are found around comparatively hot (Teff > 5900 K) and massive (MA > 1.15 Msun) stars. Chromospheric activity indicators are available for eight of these stars, and all imply a low activity level. The planets have small or zero orbital eccentricities, so tidal effects struggle to explain their large radii. The observed dearth of large planets around small stars may be natural but could also be due to observational biases against deep transits, if these are mistakenly labelled as false positives and so not followed up.