1. Immunochemical detection of sequence-specific modifications to DNA induced by UV light
- Author
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Helen R. Griffiths, Nalini Mistry, Karl E. Herbert, and Joseph Lunec
- Subjects
Cancer Research ,Base Sequence ,Poly T ,Ultraviolet Rays ,Oligonucleotide ,Immunochemistry ,Nucleic acid sequence ,Pyrimidine dimer ,DNA ,General Medicine ,Molecular biology ,Epitope ,Thymine ,Epitopes ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Ultraviolet light ,Animals ,Rabbits ,Cytosine ,DNA Damage - Abstract
Sequence specificity of antibodies to UV-damaged DNA has not been described previously. The antisera investigated here were specific for UV-modified DNA and were absolutely dependent upon the presence of thymine residues. Using a series of oligonucleotides in competition ELISA, increased inhibition was observed with increasing chain length of UV-polythymidylate. A minimum of three adjacent thymines was required for effective inhibition; alone, dimers of thymine were poor antigens. Although UV-irradiated poly(dC) was not antigenic, cytosines could partially replace thymines within the smallest effective epitope (T-T-T) with a high degree of sequence specificity, not previously described. The main epitope induced by UV was formed from adjacent thymines and either a 3' or a 5' pyrimidine.
- Published
- 1994
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