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Start Over Author "M. S. c." Publisher oxford university press (oup)
25 results on '"M. S. c."'

2. The study on optogenetic tachyarrhythmia termination under pathological conditions from the single cell to the whole heart

Author

M S C Fontes, A A F De Vries, Cindy I. Bart, M.J. Schalij, T J Van Brakel, Balázs Ördög, T Van Den Heuvel, E.C.A. Nyns, and Daniël A. Pijnappels

Subjects
medicine.anatomical_structure, business.industry, Cell, Medicine, Optogenetics, Cardiology and Cardiovascular Medicine, business, Neuroscience, Pathological
Abstract

Background Ventricular tachyarrhytmias (VTs) are common among patients suffering from cardiac remodeling and cause significant morbidity and mortality. Current research and treatment options for such VTs are suboptimal, hence new strategies are urgently needed. Optogenetics offers efficacious means to control cardiac rhythm, including shock-free VT termination. However, this has not been demonstrated in diseased hearts in vivo, while clinical translation would not only require such demonstration, but also an in-depth understanding of cellular responses. Purpose To assess the optogenetic response at the cardiac cell, tissue, and whole heart level in terms of rhtyhm control under pathological conditions by an integrative experimental platform including in vitro and in vivo models of cardiac disease. Methods Remodeling was induced in neonatal rat ventricular cardiomyocytes (NRVMs) by phenylephrine (PE) exposure. Pathological conditions leading to ventricular remodeling were mimicked by transverse aortic constriction (TAC) surgery in adult rats. The light-activated ion channel ReaChR was ectopically expressed in NRVMs and in hearts of TAC and sham animals by viral vector-based gene delivery. Results Electrical and structural remodeling was evidenced by elongated action potential durations (p0.05). Illumination (1 s) caused a sudden shift in membrane potential leading to a plateau at −7.3 mV for PE-treated and −18.9 mV for CTL cells (p>0.05). Hearts explanted from TAC animals showed increased average heart weight to body weight ratio, ventricular fibrosis and expression of hypertrophy markers (ANP, aSkMA, p0.05) in VMs from TAC and sham animals, which currents led to comparable shifts in membrane potential (65.3 mV for TAC and 63.9 mV for CTL). In line with this, illumination caused marked depolarization in tissue preparations (from −77.6 to −16.4 mV) in TAC animals as assessed by conventional sharp electrode measurements. Importantly, as anticipated, electrically-induced VT episodes could be terminated in open chest experiments in TAC animals (n=6; 76.3% of cases) by epicardial illumination in vivo. Conclusions Key operational parameters of the optogenetic response remained unaffected in models of cardiac disease, which allowed efficacious optogenetic VT termination in the diseased rat heart exhibiting structural and electrical remodeling. These findings corroborate the translational potential of shock-free therapy of cardiac arrhythmia by optogenetics. Funding Acknowledgement Type of funding source: Public grant(s) – EU funding. Main funding source(s): This work was supported by personal funding from the Netherlands Organization for Scientific Research (NWO, Vidi grant 1714336 to D.A.P.). D.A.P. is also a recipient of the European Research Council (ERC), Starting grant (716509). Additional support was provided by the Netherlands Heart Institute (ICIN grant 230.148-04 to A.A.F.d.V.). more...

Published
2020
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3. Maternal SARS-CoV-2 Infection Associated to Systemic Inflammatory Response and Pericardial Effusion in the Newborn: A Case Report

Author

Lima, Andressa R O, primary, Cardoso, Cynthia C, additional, Bentim, Priscilla R B, additional, Voloch, Carolina M, additional, Rossi, Átila D, additional, da Costa, Raissa Mirella M S C, additional, da Paz, Juliana Aparecida S, additional, Agostinho, Rafael F, additional, Figueiredo, Valéria R F S, additional, Júnior, Jarba S S, additional, de Almeida, Luiz G P, additional, Gerber, Alexandra L, additional, Abuassi, Clarissa A, additional, Rodrigues, Natalia F, additional, Tanuri, Amilcar, additional, Bozza, Patricia T, additional, Bastos, Cesar S, additional, de Vasconcelos, Ana Tereza R, additional, Kruger, Stella Beatriz, additional, Vallim, Giovanna Geórgia P C A, additional, Nishihara, Roberto J, additional, Barroso, Shana Priscila C, additional, and Morrot, Alexandre, additional more...

Published
2020
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5. Density of Upper Respiratory Colonization With Streptococcus pneumoniae and Its Role in the Diagnosis of Pneumococcal Pneumonia Among Children Aged <5 Years in the PERCH Study

Author

Baggett, Henry C, Watson, Nora L, Deloria Knoll, Maria, Brooks, W Abdullah, Feikin, Daniel R, Hammitt, Laura L, Howie, Stephen R C, Kotloff, Karen L, Levine, Orin S, Madhi, Shabir A, Murdoch, David R, Scott, J Anthony G, Thea, Donald M, Antonio, Martin, Awori, Juliet O, Baillie, Vicky L, DeLuca, Andrea N, Driscoll, Amanda J, Duncan, Julie, Ebruke, Bernard E, Goswami, Doli, Higdon, Melissa M, Karron, Ruth A, Moore, David P, Morpeth, Susan C, Mulindwa, Justin M, Park, Daniel E, Paveenkittiporn, Wantana, Piralam, Barameht, Prosperi, Christine, Sow, Samba O, Tapia, Milagritos D, Zaman, Khalequ, Zeger, Scott L, O’Brien, Katherine L, O, K L, L, O S, K, M D, F, D R, D, A N, D, A J, Fancourt, Nicholas, Fu, Wei, H, L L, H, M M, Wangeci Kagucia, E, K, R A, Li, Mengying, P, D E, P, C, Wu, Zhenke, Z, S L, W, N L, Crawley, Jane, M, D R, B, W A, Endtz, Hubert P, Z, K, G, D, Hossain, Lokman, Jahan, Yasmin, Ashraf, Hasan, C H, S R, E, B E, A, M, McLellan, Jessica, Machuka, Eunice, Shamsul, Arifin, Zaman, Syed M A, Mackenzie, Grant, G S, J A, A, J O, M, S C, Kamau, Alice, Kazungu, Sidi, Ominde, Micah Silaba, K, K L, T, M D, S, S O, Sylla, Mamadou, Tamboura, Boubou, Onwuchekwa, Uma, Kourouma, Nana, Toure, Aliou, M, S A, M, D P, Adrian, Peter V, B, V L, Kuwanda, Locadiah, Mudau, Azwifarwi, Groome, Michelle J, Mahomed, Nasreen, B, H C, Thamthitiwat, Somsak, Maloney, Susan A, Bunthi, Charatdao, Rhodes, Julia, Sawatwong, Pongpun, Akarasewi, Pasakorn, T, D M, Mwananyanda, Lawrence, Chipeta, James, Seidenberg, Phil, Mwansa, James, wa Somwe, Somwe, Kwenda, Geoffrey, Anderson, Trevor P, and Mitchell, Joanne more...

Subjects
0301 basic medicine, Microbiology (medical), Male, medicine.medical_specialty, 030106 microbiology, Zambia, medicine.disease_cause, Mali, Polymerase Chain Reaction, 03 medical and health sciences, South Africa, 0302 clinical medicine, Internal medicine, Streptococcus pneumoniae, medicine, Humans, Colonization, 030212 general & internal medicine, Respiratory Tract Infections, Bacteriological Techniques, Bangladesh, Respiratory tract infections, business.industry, Case-control study, Child Health, Infant, Newborn, Infant, Pneumonia, Pneumococcal, medicine.disease, Thailand, Kenya, Bacterial Load, 3. Good health, Surgery, respiratory tract diseases, Pneumonia, Infectious Diseases, Specimen collection, Case-Control Studies, Child, Preschool, Pneumococcal pneumonia, Etiology, Female, Gambia, Supplement Article, business, Child, Hospitalized
Abstract

Previous studies suggested an association between upper airway pneumococcal colonization density and pneumococcal pneumonia, but data in children are limited. Using data from the Pneumonia Etiology Research for Child Health (PERCH) study, we assessed this potential association.PERCH is a case-control study in 7 countries: Bangladesh, The Gambia, Kenya, Mali, South Africa, Thailand, and Zambia. Cases were children aged 1-59 months hospitalized with World Health Organization-defined severe or very severe pneumonia. Controls were randomly selected from the community. Microbiologically confirmed pneumococcal pneumonia (MCPP) was confirmed by detection of pneumococcus in a relevant normally sterile body fluid. Colonization density was calculated with quantitative polymerase chain reaction analysis of nasopharyngeal/oropharyngeal specimens.Median colonization density among 56 cases with MCPP (MCPP cases; 17.28 × 106 copies/mL) exceeded that of cases without MCPP (non-MCPP cases; 0.75 × 106) and controls (0.60 × 106) (each P.001). The optimal density for discriminating MCPP cases from controls using the Youden index was6.9 log10 copies/mL; overall, the sensitivity was 64% and the specificity 92%, with variable performance by site. The threshold was lower (≥4.4 log10 copies/mL) when MCPP cases were distinguished from controls who received antibiotics before specimen collection. Among the 4035 non-MCPP cases, 500 (12%) had pneumococcal colonization density6.9 log10 copies/mL; above this cutoff was associated with alveolar consolidation at chest radiography, very severe pneumonia, oxygen saturation92%, C-reactive protein ≥40 mg/L, and lack of antibiotic pretreatment (all P.001).Pneumococcal colonization density6.9 log10 copies/mL was strongly associated with MCPP and could be used to improve estimates of pneumococcal pneumonia prevalence in childhood pneumonia studies. Our findings do not support its use for individual diagnosis in a clinical setting. more...

Published
2017
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6. P5717Biological shock-free termination of ventricular tachyarrhythmias in the adult rat model of cardiac pressure overload

Author

E.C.A. Nyns, M S C Fontes, A. Kip, Cindy I. Bart, A A F De Vries, L Volkers, and D A Pijnappels

Subjects
Pressure overload, medicine.medical_specialty, business.industry, Ventricular Tachyarrhythmias, Internal medicine, Shock (circulatory), Rat model, medicine, Cardiology, medicine.symptom, Cardiology and Cardiovascular Medicine, business
Published
2018
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8. Unenhanced CT imaging is highly sensitive to exclude pheochromocytoma: a multicenter study

Author

Buitenwerf, Edward, primary, Korteweg, Tijmen, additional, Visser, Anneke, additional, Haag, Charlotte M S C, additional, Feelders, Richard A, additional, Timmers, Henri J L M, additional, Canu, Letizia, additional, Haak, Harm R, additional, Bisschop, Peter H L T, additional, Eekhoff, Elisabeth M W, additional, Corssmit, Eleonora P M, additional, Krak, Nanda C, additional, Rasenberg, Elise, additional, van den Bergh, Janneke, additional, Stoker, Jaap, additional, Greuter, Marcel J W, additional, Dullaart, Robin P F, additional, Links, Thera P, additional, and Kerstens, Michiel N, additional more...

Published
2018
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9. Andrology

Author

M. S. C. Carchenilla, D. Agudo, S. Rubio, D. Becerra, F. Bronet, J. A. Garcia-Velasco, A. Pacheco, M. Lardone, A. Piottante, A. Parada-Bustamante, F. Argandona, M. Florez, A. Espinoza, M. Ebensperger, A. Castro, M. Cohen-Bacrie, S. Belloc, A. Dalleac, E. Amar, V. Izard, A. Hazout, P. Cohen-Bacrie, J. de Mouzon, F. Muzzonigro, A. M. Crivello, I. Stanghellini, L. Bernardini, A. P. Ferraretti, C. Magli, L. Gianaroli, P. S. Martin, M. H. Duvison, M. D. Silva, J. Gosalvez, F. S. Martin, A. Pomante, F. Colombo, M. Mattioli, B. Barboni, M. C. Magli, O. Hacifazlioglu, N. Findikli, U. Goktolga, M. Bahceci, A. Jakab, A. Mokanszki, A. Varga, M. Benyo, Z. Kassai, E. Olah, Z. Molnar, G. I. Gundogan, H. H. Bozkurt, T. Irez, A. Domingo, C. Anarte, N. Presilla, I. Calvo, O. Aguirre, A. Oroquieta, J. A. Agirregoikoa, J. L. De Pablo, G. Barrenetxea, I. Moragues, M. L. Medrano, A. Montoya, B. Ramos, M. J. G. Torres, J. Aizpurua, S. R. Ibala, H. Ghedir, A. Mehri, I. Zidi, S. Brahem, M. Mehdi, M. Ajina, A. Saad, M. J. Gomez-Torres, J. E. Cavaco, L. Rato, M. G. Alves, T. R. Dias, G. Lopes, S. Socorro, P. F. Oliveira, A. M. Lobascio, M. G. Minasi, E. Greco, M. Bungum, A. Bungum, N. Silver, M. Zahiri, M. Movahedin, S. J. Mowla, M. Noruzinia, M. Huleihel, Y. Abarbanel, E. P. Haber, M. Azab, D. Lan, E. Lunenfeld, M. J. Smith, Q. V. Neri, L. Harvey, Z. Rosenwaks, G. D. Palermo, M. Alhalabi, S. Samawi, H. Droubi, M. Khalaf, A. Taha, R. Khatib, A. Bednarowska-flisiak, M. Wcislo, J. Liss, A. Swider, J. Szczyglinska, M. Grzymkowska, A. Bruszczynska, J. Glowacka, K. Kitowska-Marszalkowska, M. Krapchev, A. Mirecka, K. Wisniewska, K. Lukaszuk, I. Natali, L. Tamburrino, M. Cambi, S. Marchiani, I. Noci, M. Maggi, G. Forti, E. Baldi, M. Muratori, X. Ferraretto, B. Pasquet, F. Damond, S. Matheron, S. Epelboin, S. Yahi, P. Demailly, N. Rougier, C. Yazbeck, L. Delaroche, P. Longuet, M. Llabador, C. Estellat, C. Patrat, M. Askarijahromi, M. Amanlu, S. j. Mowla, Z. Mazaheri, P. Christensen, E. S. Sills, R. Fischer, O. G. J. Naether, D. Walsh, K. Rudolf, G. Coull, V. Baukloh, R. Labouriau, A. Birck, F. Parisi, B. Parrilla, M. Oneta, V. Savasi, L. Veleva, T. Milachich, I. Bochev, I. Antonova, A. Shterev, V. Vlaisavljevic, B. P. Breznik, B. Kovacic, M. Serrano, M. C. Gonzalvo, A. Clavero, M. F. Fernandez, J. Mozas, L. Martinez, J. Fontes, S. Carrillo, M. L. Lopez-Regalado, B. Lopez-Leria, I. Orozco, A. Mantilla, J. A. Castilla, G. Mskhalaya, E. Zakharova, V. Zaletova, E. Kasatonova, Y. Melnik, E. Efremov, M. C. Schiewe, G. Verheyen, H. Tournaye, I. Phletincx, C. A. Sims, C. Rothman, E. Borges, A. S. Setti, D. P. A. F. Braga, L. Vingris, A. Iaconelli, C. Dupont, C. Faure, N. Sermondade, B. Gautier, C. Herbemont, I. Aknin, J. P. Klein, I. Cedrin-Durnerin, J. P. Wolf, S. Czernichow, R. Levy, C. Rondanino, C. Chauffour, L. Ouchchane, C. Artonne, L. Janny, J. M. Lobaccaro, D. H. Volle, F. Brugnon, N. Colacurci, P. Piomboni, G. Ruvolo, F. Lombardo, E. L. Verde, V. De Leo, M. Lispi, E. Papaleo, R. De Palo, L. Gandini, S. Longobardi, Y. Yokota, M. Yokota, H. Yokota, Y. Araki, S. Alshahrani, D. Durairajanayagam, R. Sharma, E. Sabanegh, A. Agarwal, H. Hattori, Y. Nakajo, T. Ikeno, Y. Sato, T. Kyoya, K. Kyono, B. Li, J. B. Li, X. F. Xiao, Y. F. Ma, J. Wang, X. X. Liang, H. X. Zhao, F. Jiang, Y. Q. Yao, X. H. Wang, N. R. Roan, H. Liu, J. Muller, A. Avila-Herrera, K. S. Pollard, P. Lishko, F. Kirchhoff, J. Munch, H. E. Witkowska, W. C. Greene, A. Mangiarini, A. Paffoni, L. Restelli, C. Guarneri, E. Somigliana, G. Ragni, R. Bou, M. Aleman, F. Guardiola, C. Camargo, J. B. A. Oliveira, C. G. Petersen, A. L. Mauri, F. C. Massaro, A. Nicoletti, A. M. Nascimento, L. D. Vagnini, A. M. V. C. Martins, M. Cavagna, R. L. R. Baruffi, and J. G. Franco more...

Subjects
Reproductive Medicine, Apoptosis, Rehabilitation, Varicocele, medicine, Obstetrics and Gynecology, Biology, medicine.disease, Sperm, Function (biology), Chromatin, Cell biology
Published
2013
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12. Andrology

Author

Carchenilla, M. S. C., primary, Agudo, D., additional, Rubio, S., additional, Becerra, D., additional, Bronet, F., additional, Garcia-Velasco, J. A., additional, Pacheco, A., additional, Lardone, M., additional, Piottante, A., additional, Parada-Bustamante, A., additional, Argandona, F., additional, Florez, M., additional, Espinoza, A., additional, Ebensperger, M., additional, Castro, A., additional, Cohen-Bacrie, M., additional, Belloc, S., additional, Dalleac, A., additional, Amar, E., additional, Izard, V., additional, Hazout, A., additional, Cohen-Bacrie, P., additional, de Mouzon, J., additional, Muzzonigro, F., additional, Crivello, A. M., additional, Stanghellini, I., additional, Bernardini, L., additional, Ferraretti, A. P., additional, Magli, C., additional, Gianaroli, L., additional, Martin, P. S., additional, Duvison, M. H., additional, Silva, M. D., additional, Gosalvez, J., additional, Martin, F. S., additional, Pomante, A., additional, Colombo, F., additional, Mattioli, M., additional, Barboni, B., additional, Magli, M. C., additional, Hacifazlioglu, O., additional, Findikli, N., additional, Goktolga, U., additional, Bahceci, M., additional, Jakab, A., additional, Mokanszki, A., additional, Varga, A., additional, Benyo, M., additional, Kassai, Z., additional, Olah, E., additional, Molnar, Z., additional, Gundogan, G. I., additional, Bozkurt, H. H., additional, Irez, T., additional, Domingo, A., additional, Anarte, C., additional, Presilla, N., additional, Calvo, I., additional, Aguirre, O., additional, Oroquieta, A., additional, Agirregoikoa, J. A., additional, De Pablo, J. L., additional, Barrenetxea, G., additional, Moragues, I., additional, Medrano, M. L., additional, Montoya, A., additional, Ramos, B., additional, Torres, M. J. G., additional, Aizpurua, J., additional, Ibala, S. R., additional, Ghedir, H., additional, Mehri, A., additional, Zidi, I., additional, Brahem, S., additional, Mehdi, M., additional, Ajina, M., additional, Saad, A., additional, Gomez-Torres, M. J., additional, Cavaco, J. E., additional, Rato, L., additional, Alves, M. G., additional, Dias, T. R., additional, Lopes, G., additional, Socorro, S., additional, Oliveira, P. F., additional, Lobascio, A. M., additional, Minasi, M. G., additional, Greco, E., additional, Bungum, M., additional, Bungum, A., additional, Silver, N., additional, Zahiri, M., additional, Movahedin, M., additional, Mowla, S. J., additional, Noruzinia, M., additional, Huleihel, M., additional, Abarbanel, Y., additional, Haber, E. P., additional, Azab, M., additional, Lan, D., additional, Lunenfeld, E., additional, Smith, M. J., additional, Neri, Q. V., additional, Harvey, L., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Alhalabi, M., additional, Samawi, S., additional, Droubi, H., additional, Khalaf, M., additional, Taha, A., additional, Khatib, R., additional, Bednarowska-flisiak, A., additional, Wcislo, M., additional, Liss, J., additional, Swider, A., additional, Szczyglinska, J., additional, Grzymkowska, M., additional, Bruszczynska, A., additional, Glowacka, J., additional, Kitowska-Marszalkowska, K., additional, Krapchev, M., additional, Mirecka, A., additional, Wisniewska, K., additional, Lukaszuk, K., additional, Natali, I., additional, Tamburrino, L., additional, Cambi, M., additional, Marchiani, S., additional, Noci, I., additional, Maggi, M., additional, Forti, G., additional, Baldi, E., additional, Muratori, M., additional, Ferraretto, X., additional, Pasquet, B., additional, Damond, F., additional, Matheron, S., additional, Epelboin, S., additional, Yahi, S., additional, Demailly, P., additional, Rougier, N., additional, Yazbeck, C., additional, Delaroche, L., additional, Longuet, P., additional, Llabador, M., additional, Estellat, C., additional, Patrat, C., additional, Askarijahromi, M., additional, Amanlu, M., additional, Mowla, S. j., additional, Mazaheri, Z., additional, Christensen, P., additional, Sills, E. S., additional, Fischer, R., additional, Naether, O. G. J., additional, Walsh, D., additional, Rudolf, K., additional, Coull, G., additional, Baukloh, V., additional, Labouriau, R., additional, Birck, A., additional, Parisi, F., additional, Parrilla, B., additional, Oneta, M., additional, Savasi, V., additional, Veleva, L., additional, Milachich, T., additional, Bochev, I., additional, Antonova, I., additional, Shterev, A., additional, Vlaisavljevic, V., additional, Breznik, B. P., additional, Kovacic, B., additional, Serrano, M., additional, Gonzalvo, M. C., additional, Clavero, A., additional, Fernandez, M. F., additional, Mozas, J., additional, Martinez, L., additional, Fontes, J., additional, Carrillo, S., additional, Lopez-Regalado, M. L., additional, Lopez-Leria, B., additional, Orozco, I., additional, Mantilla, A., additional, Castilla, J. A., additional, Mskhalaya, G., additional, Zakharova, E., additional, Zaletova, V., additional, Kasatonova, E., additional, Melnik, Y., additional, Efremov, E., additional, Schiewe, M. C., additional, Verheyen, G., additional, Tournaye, H., additional, Phletincx, I., additional, Sims, C. A., additional, Rothman, C., additional, Borges, E., additional, Setti, A. S., additional, Braga, D. P. A. F., additional, Vingris, L., additional, Iaconelli, A., additional, Dupont, C., additional, Faure, C., additional, Sermondade, N., additional, Gautier, B., additional, Herbemont, C., additional, Aknin, I., additional, Klein, J. P., additional, Cedrin-Durnerin, I., additional, Wolf, J. P., additional, Czernichow, S., additional, Levy, R., additional, Rondanino, C., additional, Chauffour, C., additional, Ouchchane, L., additional, Artonne, C., additional, Janny, L., additional, Lobaccaro, J. M., additional, Volle, D. H., additional, Brugnon, F., additional, Colacurci, N., additional, Piomboni, P., additional, Ruvolo, G., additional, Lombardo, F., additional, Verde, E. L., additional, De Leo, V., additional, Lispi, M., additional, Papaleo, E., additional, De Palo, R., additional, Gandini, L., additional, Longobardi, S., additional, Yokota, Y., additional, Yokota, M., additional, Yokota, H., additional, Araki, Y., additional, Alshahrani, S., additional, Durairajanayagam, D., additional, Sharma, R., additional, Sabanegh, E., additional, Agarwal, A., additional, Hattori, H., additional, Nakajo, Y., additional, Ikeno, T., additional, Sato, Y., additional, Kyoya, T., additional, Kyono, K., additional, Li, B., additional, Li, J. B., additional, Xiao, X. F., additional, Ma, Y. F., additional, Wang, J., additional, Liang, X. X., additional, Zhao, H. X., additional, Jiang, F., additional, Yao, Y. Q., additional, Wang, X. H., additional, Roan, N. R., additional, Liu, H., additional, Muller, J., additional, Avila-Herrera, A., additional, Pollard, K. S., additional, Lishko, P., additional, Kirchhoff, F., additional, Munch, J., additional, Witkowska, H. E., additional, Greene, W. C., additional, Mangiarini, A., additional, Paffoni, A., additional, Restelli, L., additional, Guarneri, C., additional, Somigliana, E., additional, Ragni, G., additional, Bou, R., additional, Aleman, M., additional, Guardiola, F., additional, Camargo, C., additional, Oliveira, J. B. A., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Nicoletti, A., additional, Nascimento, A. M., additional, Vagnini, L. D., additional, Martins, A. M. V. C., additional, Cavagna, M., additional, Baruffi, R. L. R., additional, and Franco, J. G., additional more...

Published
2013
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20. ELECTRON MICROSCOPY OF MELANOCYTES

Author

M. S. C. Birbeck

Subjects
Melanins, Microscopy, Materials science, Electrons, General Medicine, Electron, Chromatophore, law.invention, Melanin, Microscopy, Electron, law, Biophysics, Humans, Melanocytes, Chromatophores, Electron microscope
Published
1962
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21. Hypomethylation of DNA in Human Cancer Cells: A Site-Specific Change in the c-myc Oncogene<xref ref-type='fn' rid='FN2'>2</xref>

Author

R. M. Hoffman, M. S. C. Cheah, and C. D. Wallace

Subjects
Cancer Research, HpaII, Methylation, Biology, medicine.disease_cause, Molecular biology, chemistry.chemical_compound, Exon, genomic DNA, Oncology, chemistry, DNA methylation, medicine, Cancer research, Carcinogenesis, Cytosine, DNA
Abstract

Site-specific methylation of the human c-myc oncogene was investigated in a set of cultured human tumor cell lines and normal human fibroblast strains. As previously reported, all of the tumor cell lines in contrast to normal cells were hypomethylated to various degrees in their total genomic DNA. The presence of methylation in specific regions of the c-myc gene was analyzed by use of the restriction endonuclease isoschizomers MspI and HpaII, which recognize the sequence 5'-CCGG-3' (CCGG = DNA sequence of 2 cytosine bases followed by 2 guanine bases) but differ in their abilities to cleave at the internal cytosine residue when it is methylated. The first exon, first intervening sequence, and the second exon were hypomethylated in all cell types, regardless of whether the cells were normal or oncogenically transformed and regardless of the degree of total genomic methylation of the cell. However, the solitary CCGG site in the third exon was fully methylated in normal cell strains. In contrast, in 3 of 5 tumor cell lines measured, this site was hypomethylated. This is the first demonstration of a site-specific DNA methylation defect in a cellular oncogene. Some possible implications relating disruption of DNA methylation to oncogene control and oncogenesis are discussed. more...

Published
1984
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