1. Rapid, convenient radioimmunoassay of estrone sulfate
- Author
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S. Patel, M. Javad Khosravi, Terry Gimpel, Kalyani Damodaran, V. Daniel Castracane, Frank Z. Stanczyk, Girish N. Ranadive, Jehangir Mistry, and Anastasia Diamandi
- Subjects
medicine.medical_specialty ,medicine.drug_class ,media_common.quotation_subject ,Biochemistry (medical) ,Clinical Biochemistry ,Estrone ,Radioimmunoassay ,Biology ,Luteal phase ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Estrone sulfate ,Estrogen ,Internal medicine ,Follicular phase ,Blood plasma ,medicine ,Menstrual cycle ,media_common - Abstract
We developed a specific, simple, and rapid RIA for the direct quantification of estrone sulfate (E1S) and established its performance characteristics. The assay has a dynamic range of 0.05–90 μg/L with a detection limit of 0.009 μg/L. Intraassay CVs were 9.2%, 4.5%, and 4.6% at 0.35, 9.0, and 60 μg/L, respectively. Interassay CVs were 8.8%, 5.1%, and 5.5% at 0.076, 0.5, and 12 μg/L, respectively. Linearity of dilution studies showed values of 80–105% of expected, and recovery of E1S added to serum samples ranged from 82% to 102%. Cross-reactivities with structurally related estrogens were
- Published
- 1998
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