1. The phylogenetic relationships ofChlorobium tepidumandChloroflexus aurantiacusbased upon their RecA sequences
- Author
-
Kurt Gish, Donald A. Bryant, Tanja M. Gruber, and Jonathan A. Eisen
- Subjects
Flexibacter ,Molecular Sequence Data ,Restriction Mapping ,Chlorobium ,Microbiology ,Article ,Chlorobi ,Phylogenetics ,Genetics ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Phylogeny ,biology ,Phylogenetic tree ,Chloroflexus aurantiacus ,Sequence Analysis, DNA ,biology.organism_classification ,Rec A Recombinases ,Cytophaga ,Chlorobium tepidum ,Genes, Bacterial ,Molecular phylogenetics ,bacteria ,Sequence Alignment - Abstract
Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteriodes group, a relationship supported by results obtained with several phylogenetic markers. Cf. aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram-positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.
- Published
- 1998