1. Synthesis and hybridization properties of inverse oligonucleotides
- Author
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Mirella Marangoni, Arthur Van Aerschot, Jef Rozenski, Piet Herdewijn, and Patrick Augustyns
- Subjects
Inversion, Chromosome ,Phosphoramidite ,Oligonucleotide ,Oligonucleotides ,Temperature ,Nucleic Acid Hybridization ,RNA ,Inverse ,Biology ,Combinatorial chemistry ,Thymine ,chemistry.chemical_compound ,Nucleic acid thermodynamics ,Monomer ,Biochemistry ,chemistry ,Chromosome Inversion ,Genetics ,Nucleic Acid Conformation ,DNA ,Research Article - Abstract
The synthesis of adenine and thymine cyclopentylethyl nucleosides is presented. This novel constrained monomeric building block is very difficult to incorporate into oligonucleotides. It was introduced in 13mer oligodeoxynucleotide sequences at a single position using H-phosphonate chemistry. Phosphoramidite chemistry completely failed in this particular case. The H-phosphonate building blocks were obtained starting from the corresponding phosphoramidites. Stability of duplexes with RNA and DNA is significantly reduced. ispartof: Nucleic acids research vol:25 issue:15 pages:3034-3041 ispartof: location:England status: published
- Published
- 1997