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Your search keyword '"Dierikx, Cindy M."' showing total 8 results
Start Over Author "Dierikx, Cindy M." Publisher oxford university press (oup)
8 results on '"Dierikx, Cindy M."'

3. Long-term Carriage of Extended-Spectrum β-Lactamase–Producing Escherichia coli and Klebsiella pneumoniae in the General Population in The Netherlands

Author

van Duijkeren, Engeline, Wielders, Cornelia C H, Dierikx, Cindy M, van Hoek, Angela H A M, Hengeveld, Paul, Veenman, Christiaan, Florijn, Alice, Lotterman, Aniek, Smit, Lidwien A M, van Dissel, Jaap T, Maassen, Catharina B M, de Greeff, Sabine C, dIRAS RA-I&I RA, One Health Microbieel, Dep Infectieziekten Immunologie, dIRAS RA-I&I RA, One Health Microbieel, and Dep Infectieziekten Immunologie

Subjects
Male, 0301 basic medicine, antibiotic resistance, ST131, Klebsiella pneumoniae, medicine.disease_cause, Polymerase Chain Reaction, law.invention, Feces, 0302 clinical medicine, Plasmid, Risk Factors, law, Surveys and Questionnaires, Genotype, polycyclic compounds, Longitudinal Studies, 030212 general & internal medicine, Escherichia coli Infections, Phylogeny, Polymerase chain reaction, Netherlands, education.field_of_study, biology, Middle Aged, Bacterial Typing Techniques, Infectious Diseases, Carrier State, Female, Adult, Microbiology (medical), 030106 microbiology, Population, Microbial Sensitivity Tests, beta-Lactamases, Microbiology, Young Adult, pAmpC, 03 medical and health sciences, Bacterial Proteins, Escherichia coli, medicine, Humans, education, carriage, business.industry, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, Klebsiella Infections, Cross-Sectional Studies, Carriage, ESBL, bacteria, Multilocus sequence typing, business, Multilocus Sequence Typing
Abstract

Background This longitudinal study aimed to investigate (risk factors for) persistence of carriage and molecular characteristics of extended-spectrum and plasmid-encoded AmpC β-lactamase-producing (ESBL/pAmpC) Escherichia coli and Klebsiella pneumoniae (ESBL-E/K) in adults in the Dutch community. Methods Following a cross-sectional study (ESBL-E/K prevalence, 4.5%), a subset of ESBL-E/K-positive (n = 76) and -negative (n = 249) individuals volunteered to provide 5 monthly fecal samples and questionnaires. ESBL-E/K was cultured using selective enrichment/culture, and multilocus sequence types (MLSTs) were determined. ESBL/pAmpC-genes were analyzed using polymerase chain reaction (PCR) and sequencing. Plasmids were characterized and subtyped by plasmid MLST. Risk factors for persistent carriage were analyzed using logistic regression. Results Of the initially ESBL-E/K-positive participants, 25 of 76 (32.9%) remained positive in all subsequent samples; 51 of 76 persons (67.1%) tested ESBL-E/K negative at some time point during follow-up, of which 31 (40.8%) stayed negative throughout the longitudinal study. Carriers often carried the same ESBL gene and plasmid, but sometimes in different ESBL-E/K strains, indicative for horizontal transfer of plasmids. Of the 249 initially ESBL-E/K-negative participants, the majority (n = 218 [87.6%]) tested negative during 8 months of follow-up, whereas 31 of 249 (12.4%) participants acquired an ESBL-E/K. Escherichia coli phylogenetic group B2 and D and travel to ESBL high-prevalence countries were associated with prolonged carriage. Conclusions ESBL-E/K carriage persisted for >8 months in 32.9% of the initially ESBL-positive individuals, while 12.4% of initially negative individuals acquired ESBL-E/K during the study. A single positive test result provides no accurate prediction for prolonged carriage. Acquisition/loss of ESBL-E/K does not seem to be a random process, but differs between bacterial genotypes.

Published
2017
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6. Molecular relatedness of ESBL/AmpC-producing Escherichia coli from humans, animals, food and the environment: a pooled analysis

Author

Dorado-García, Alejandro, primary, Smid, Joost H, additional, van Pelt, Wilfrid, additional, Bonten, Marc J M, additional, Fluit, Ad C, additional, van den Bunt, Gerrita, additional, Wagenaar, Jaap A, additional, Hordijk, Joost, additional, Dierikx, Cindy M, additional, Veldman, Kees T, additional, de Koeijer, Aline, additional, Dohmen, Wietske, additional, Schmitt, Heike, additional, Liakopoulos, Apostolos, additional, Pacholewicz, Ewa, additional, Lam, Theo J G M, additional, Velthuis, Annet G, additional, Heuvelink, Annet, additional, Gonggrijp, Maaike A, additional, van Duijkeren, Engeline, additional, van Hoek, Angela H A M, additional, de Roda Husman, Ana Maria, additional, Blaak, Hetty, additional, Havelaar, Arie H, additional, Mevius, Dik J, additional, and Heederik, Dick J J, additional

Published
2017
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