1. Establishment and Characterization of Factor-Dependent Macrophage Cell Lines
- Author
-
Kazunori Ohki and Ariaki Nagayama
- Subjects
medicine.medical_specialty ,Immunology ,Biology ,Cell Line ,Mice ,Colony-Stimulating Factors ,Internal medicine ,medicine ,Animals ,Immunology and Allergy ,Macrophage ,Receptor ,Calcimycin ,Interleukin 4 ,Interleukin 3 ,Cell growth ,Macrophages ,Drug Synergism ,Cell Biology ,Colony-stimulating factor ,Culture Media ,Cell biology ,Endocrinology ,Cell culture ,Tetradecanoylphorbol Acetate ,Interleukin-3 ,Signal transduction ,Cell Division - Abstract
Three macrophage cell lines from bone marrow cells of C3H/HeN mice were isolated by successive transfer of the cells in culture with L-cell-conditioned medium (LCM) or WEHI-3 cell-conditioned medium (WEHI-3CM). These cell lines, which express Fc receptors, are involved in Fc-mediated phagocytosis and possess nonspecific esterase activity. Two (BDM-1 and BDM-2) of three cell lines show dependency for growth on either macrophage colony-stimulating factor (M-CSF) (CSF-1) or granulocyte-macrophage colony-stimulating factor (GM-CSF) and do not respond to interleukin 3 (IL-3). The third clone (BDM-3) proliferates in response to IL-3 as well as to GM-CSF and weakly responds to M-CSF and to interleukin 4 (IL-4). GM-CSF, in combination with the suboptimal concentration of M-CSF, acted synergistically on the proliferation of BDM-1 cells. The tumor-promoting phorbol diester, 12-o-tetradecanoyl-phorbol-13-acetate (TPA) also acted synergistically with the three CSFs (IL-3, GM-CSF, and M-CSF) to stimulate the proliferation of BDM-1 cells. The synergistic effect was observed when cells were pretreated with TPA and subsequently stimulated with IL-3. The calcium ionophore A23187 enhanced the proliferation of BDM-1 cells costimulated with TPA and IL-3. These factor-dependent macrophage cell lines should be useful for studying signal transduction mechanisms in the regulation of cell growth.
- Published
- 1988