1. Insights about minority HIV-1 strains in transmitted drug resistance mutation dynamics and disease progression
- Author
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Ursula Castro de Oliveira, Ana Rachel Leda, Inácio de Loiola Meirelles Junqueira de Azevedo, Maria Cecília Araripe Sucupira, James Hunter, and Ricardo Sobhie Diaz
- Subjects
0301 basic medicine ,Microbiology (medical) ,Genotype ,Anti-HIV Agents ,T cell ,Population ,HIV Infections ,Drug resistance ,Biology ,medicine.disease_cause ,Cohort Studies ,03 medical and health sciences ,Proviruses ,Antiretroviral Therapy, Highly Active ,Drug Resistance, Viral ,HIV Seropositivity ,medicine ,Humans ,Pharmacology (medical) ,Seroconversion ,education ,Pharmacology ,education.field_of_study ,Mutation ,Proteolytic enzymes ,High-Throughput Nucleotide Sequencing ,Viral Load ,030112 virology ,Virology ,Reverse transcriptase ,Infectious Diseases ,medicine.anatomical_structure ,Disease Progression ,HIV-1 ,RNA, Viral ,Viral load ,Brazil - Abstract
Objectives The presence of minority transmitted drug resistance mutations was assessed using ultra-deep sequencing and correlated with disease progression among recently HIV-1-infected individuals from Brazil. Methods Samples at baseline during recent infection and 1 year after the establishment of the infection were analysed. Viral RNA and proviral DNA from 25 individuals were subjected to ultra-deep sequencing of the reverse transcriptase and protease regions of HIV-1. Results Viral strains carrying transmitted drug resistance mutations were detected in 9 out of the 25 patients, for all major antiretroviral classes, ranging from one to five mutations per patient. Ultra-deep sequencing detected strains with frequencies as low as 1.6% and only strains with frequencies >20% were detected by population plasma sequencing (three patients). Transmitted drug resistance strains with frequencies
- Published
- 2018