1. In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis
- Author
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D. H. Paper, Juergen Schoelmerich, Florian Obermeier, Nadja Dunger, K. Menzel, Werner Falk, Gerhard Rogler, Martin Hausmann, K. Balan, and Hans H Herfarth
- Subjects
Necrosis ,Colon ,medicine.medical_treatment ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Inflammatory bowel disease ,Antioxidants ,Mice ,Glucosides ,Phenols ,Weight Loss ,medicine ,Animals ,Immunology and Allergy ,Mesenteric lymph nodes ,Intestinal Mucosa ,Colitis ,Acute colitis ,Peroxidase ,Respiratory Burst ,Mice, Inbred BALB C ,business.industry ,Macrophages ,Dextran Sulfate ,Interleukin ,medicine.disease ,Respiratory burst ,Cytokine ,medicine.anatomical_structure ,Acute Disease ,Chronic Disease ,Animal Studies ,Cytokines ,Female ,Lymph Nodes ,Inflammation Mediators ,medicine.symptom ,business - Abstract
Summary Recently we demonstrated that in inflammatory bowel disease (IBD) macrophage-oxidative burst activity is increased and NADPH oxidase mRNA is induced. The herbal phenylethanoid acteoside isolated from Plantago lanceolata L. was shown to exhibit anti-oxidative potential. Using the dextran sulphate sodium (DSS)-induced colitis model, in this study we have assessed whether systemic application of acteoside affects colitis. Colitis was induced by DSS in Balb/c mice. Treatment with acteoside (120, 600 µg/mouse/day) was performed intraperitoneally. The colon lengths were determined. Colonic tissue was scored histologically (max. score 8) by a blinded investigator. T cells isolated from mesenteric lymph nodes (MLN) were stimulated with anti-CD3 antibody in the presence of interleukin (IL)-2 (final concentration 10 U/ml). After incubation for 24 h, IL-1β, IL-6, IL-12 tumour necrosis factor (TNF)-α and interferon (IFN)-γ levels in supernatants were analysed by the beadlyte® cytokine detection system. Histological scoring of colonic tissue revealed that application of acteoside was followed by a significantly improved histological score. In acute colitis the histological score was 3·2 with acteoside versus 5·2 with phosphate-buffered saline (PBS) (P
- Published
- 2007