1. Straightforward and rapid determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper with liquid chromatography and UV detection
- Author
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Jenny Lindkvist, Yngve Bergqvist, and Mikaela Malm
- Subjects
Paper ,Sulfamethoxazole ,Sulfadoxine ,Capillary action ,medicine.medical_treatment ,Rural Health ,Antimalarials ,chemistry.chemical_compound ,medicine ,Humans ,Sample preparation ,Health Education ,Antibacterial agent ,Blood Specimen Collection ,Chromatography ,Public Health, Environmental and Occupational Health ,Infant ,Sampling (statistics) ,Pneumonia ,General Medicine ,Reference Standards ,Malaria ,Infectious Diseases ,chemistry ,Child, Preschool ,Regression Analysis ,Spectrophotometry, Ultraviolet ,Parasitology ,Uv detection ,Sodium acetate ,Chromatography, Liquid ,medicine.drug - Abstract
A method for the determination of sulfadoxine and sulfamethoxazole in capillary blood on sampling paper has been developed and validated. The method is straightforward with minimal sample preparation, and is suitable for rural settings. Separation of sulfadoxine, sulfamethoxazole and internal standard was performed using a Purospher STAR RP-18 endcapped LC column (150x4.6mm) with a mobile phase consisting of acetonitrile:sodium acetate buffer pH 5.2, I=0.1 (1:2, v/v). For sulfadoxine, the within-day precision was 5.3% at 15micromol/l and 3.7% at 600micromol/l, while for sulfamethoxazole it was 5.7% at 15micromol/l and 3.8% at 600micromol/l. The lower limit of quantification was determined to 5micromol/l and precision was 5.5% and 5.0% for sulfadoxine and sulfamethoxazole, respectively.
- Published
- 2009