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Culex quinquefasciatus is an important mosquito vector responsible for the transmission of filarial worms, arthropod-borne viruses like Oropouche, St. Louis encephalitis, and West Nile and protozoans that cause avian malaria. Due to insecticide resistance documented in Cx. quinquefasciatus populations worldwide, integrated vector management programs can benefit from new strategies to control this species. The In2Care Mosquito Station (In2Care station), a commercially available dissemination station containing pyriproxyfen (PPF) and Beauveria bassiana spores, has been shown to be effective against skip-ovipositing Aedes aegypti and Aedes albopictus in previously conducted semifield and field trials. To determine the potential of Cx. quinquefasciatus adult females to autodisseminate PPF and if the In2Care station could be used for Cx. quinquefasciatus control, we assessed its efficacy in a semifield setting against wild Cx. quinquefasciatus. We found that the In2Care station was attractive to gravid Cx. quinquefasciatus females, with a significantly higher percentage of egg rafts laid in the In2Care station compared to alternative ovipots. Adult females successfully autodisseminated PPF from the In2Care station to surrounding ovipots, leading to a significant increase in mosquito emergence inhibition. Additionally, adult Cx. quinquefasciatus exposure to B. bassiana spores significantly reduced mosquito survivorship. These results suggest that the In2Care station may be effective against Cx. quinquefasciatus in addition to Ae. aegypti and Ae. albopictus. Additional field evaluations are needed to assess impacts at the population level., (© The Author(s) 2024. Published by Oxford University Press on behalf of Entomological Society of America.)

Background: Cache Valley virus (CVV) is a mosquito-borne virus that is a rare cause of disease in humans. In the fall of 2020, a patient developed encephalitis 6 weeks following kidney transplantation and receipt of multiple blood transfusions., Methods: After ruling out more common etiologies, metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) was performed. We reviewed the medical histories of the index kidney recipient, organ donor, and recipients of other organs from the same donor and conducted a blood traceback investigation to evaluate blood transfusion as a possible source of infection in the kidney recipient. We tested patient specimens using reverse-transcription polymerase chain reaction (RT-PCR), the plaque reduction neutralization test, cell culture, and whole-genome sequencing., Results: CVV was detected in CSF from the index patient by mNGS, and this result was confirmed by RT-PCR, viral culture, and additional whole-genome sequencing. The organ donor and other organ recipients had no evidence of infection with CVV by molecular or serologic testing. Neutralizing antibodies against CVV were detected in serum from a donor of red blood cells received by the index patient immediately prior to transplant. CVV neutralizing antibodies were also detected in serum from a patient who received the co-component plasma from the same blood donation., Conclusions: Our investigation demonstrates probable CVV transmission through blood transfusion. Clinicians should consider arboviral infections in unexplained meningoencephalitis after blood transfusion or organ transplantation. The use of mNGS might facilitate detection of rare, unexpected infections, particularly in immunocompromised patients., Competing Interests: Potential conflicts of interest. M. G. I. reports research support, paid to Northwestern University, from AiCuris, GSK (RSV Vaccine), Janssen, and Shire and Plumocide (antifungal); is a paid consultant for Adagio, ADMA Biologics, Takeda, AlloVir, Celltrion, Cidara, Genentech, Roche, Janssen, Shionogi, and Viracor Eurofins; is a paid member of data and safety monitoring boards for Adamis, AlloVir, CSL Behring, Janssen, Merck, SAB Biotherapeutics, Sequiris, Takeda, Talaris, and Vitaeris; and reports royalties paid to the author from UpToDate. C. Y. C. and S. M. have a patent on algorithms related to SURPI+ software, Pathogen Detection using Next-Generation Sequencing (PCT/US2016/052912). M. R. W. reports an unrelated research grant from Roche/Genentech; speaking honoraria from Takeda, Novartis, Genentech, and WebMD; payment for expert testimony from the Department of Justice; 1 patent, Method for High Percentage Recovery of Rare Cells (US-2020-0025783-A1), and 1 pending patent, Autoantibodies as a Biomarker of Paraneoplastic Encephalitis Associated with Testicular Cancer; and stock or stock options from VeriPhi Health. G. R. reports a leadership or fiduciary role as the chair of the College of American Pathologists Transfusion, Apheresis, and Cellular Therapy Committee. H. M. B. reports consulting fees from Idera (Novocure research), Myovant (Corea Therapeutics), and CARIS; payment or honoraria for lectures, presentations, speakers’ bureaus, manuscript writing, or educational events from Guardant360; and support for attending meetings and/or travel from CARIS. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed., (© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Recurrence of local transmission of Zika virus in Puerto Rico is a major public health risk to the United States, where mosquitoes Aedes aegypti (Linnaeus) and Aedes mediovittatus (Coquillett) are abundant. To determine the extent to which Ae. mediovittatus are capable of transmitting Zika virus and the influence of viremia, we evaluated infection and transmission in Ae. mediovittatus and Ae. aegypti from Puerto Rico using serial dilutions of infectious blood. Higher doses of infectious blood resulted in greater infection rates in both mosquitoes. Aedes aegypti females were up to twice as susceptible to infection than Ae. mediovittatus, indicating a more effective midgut infection barrier in the latter mosquito species. Aedes aegypti exhibited higher disseminated infection (40-95%) than Ae. mediovittatus (<5%), suggesting a substantial midgut escape barrier in Ae. mediovittatus. For Ae. aegypti, transmission rates were low over a range of doses of Zika virus ingested, suggesting substantial salivary gland barriers., (© The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Zika virus (ZIKV) first spread into Brazil in 2013 and is now present throughout the Americas. In 2016, Florida witnessed the beginnings of local ZIKV transmission. No reports of local transmission have been reported for 2018; however, travel related cases continue to be reported. Recurrence of local transmission in the United States is a major public health risk in Florida where vectors Aedes aegypti (Diptera: Culicidae, Linnaeus) and Aedes albopictus (Diptera: Culicidae, Skuse) are abundant and there is a high potential for virus reintroduction. A dose-response study was used to evaluate susceptibility and transmission potential of Florida Ae. aegypti and Ae. albopictus to ZIKV originating from Puerto Rico. Mosquitoes were orally exposed to one of three doses of ZIKV. Higher doses of infected blood resulted in overall greater infection rates in both mosquito species. Ae. aegypti and Ae. albopictus were susceptible to infection with ZIKV and revealed a significant species by dose interaction. At low doses, Ae. aegypti was significantly less susceptible to infection with ZIKV than Ae. albopictus (6.7% and 44.4%, respectively). In contrast, at high doses, Ae. aegypti was significantly more susceptible to infection than Ae. albopictus (75.8% and 53.8%, respectively). No significant differences were observed between Ae. aegypti and Ae. albopictus in disseminated infection (0-75%) and saliva infection (0-52.4%). These observations suggest greater susceptibility to infection for Ae. albopictus at lower doses likely encountered by viremic humans. However, low disseminated infection and saliva infection for Ae. albopictus, combined with catholic feeding behavior, are likely to limit transmission potential relative to Ae. aegypti., (Published by Oxford University Press on behalf of Entomological Society of America 2019.)