10 results on '"Weemaes CM"'
Search Results
2. Cutaneous graft-versus-host-like histology in childhood. Importance of clonality analysis in differential diagnosis. A case report.
- Author
-
D'hauw A, Seyger MM, Groenen PJ, Weemaes CM, Warris A, and Blokx WA
- Subjects
- Biopsy, Dermatitis, Exfoliative genetics, Diagnosis, Differential, Humans, Immunologic Deficiency Syndromes genetics, Infant, Male, Severe Combined Immunodeficiency genetics, Skin pathology, Dermatitis, Exfoliative pathology, Immunologic Deficiency Syndromes pathology, Severe Combined Immunodeficiency pathology
- Published
- 2008
- Full Text
- View/download PDF
3. Cytokine responses and regulation of interferon-gamma release by human mononuclear cells to Aspergillus fumigatus and other filamentous fungi.
- Author
-
Warris A, Netea MG, Verweij PE, Gaustad P, Kullberg BJ, Weemaes CM, and Abrahamsen TG
- Subjects
- Aspergillus fumigatus immunology, Humans, Interferon-gamma biosynthesis, Interleukin-10 biosynthesis, Monocytes drug effects, Antigens, Fungal immunology, Aspergillus fumigatus drug effects, Cytokines biosynthesis, Interferon-gamma pharmacology, Monocytes metabolism
- Abstract
There is substantial evidence that the production of proinflammatory cytokines is important in host resistance to invasive aspergillosis. Knowledge of the host response towards other filamentous fungi is scarce, as most studies have focused on Aspergillus fumigatus. In addition, interferon-gamma (IFNgamma) plays a crucial role in the control of invasive aspergillosis, but little is known about the regulation of IFNgamma after stimulation of mononuclear cells by A. fumigatus. Cytokine responses to four different Aspergillus spp., Scedosporium prolificans, and a Rhizopus oryzae strain were compared for their ability to induce the release of tumour necrosis factor-alpha (TNFalpha) and interleukin(IL)-6 by human monocytes. S. prolificans induced significantly more TNFalpha and IL-6 release compared to A. fumigatus, while the various Aspergillus spp. induce comparable levels of these cytokines. By using specific cytokine inhibitors we were able to show that endogenous IL-1, but not IL-18 and TNFalpha was required for IFNgamma and IL-10 release upon stimulation with A. fumigatus hyphae, whereas conidia induced IFNgamma stimulation is independent of these cytokines.
- Published
- 2005
- Full Text
- View/download PDF
4. Sustained viral suppression and immune recovery in HIV type 1-infected children after 4 years of highly active antiretroviral therapy.
- Author
-
Fraaij PL, Verweel G, van Rossum AM, van Lochem EG, Schutten M, Weemaes CM, Hartwig NG, Burger DM, and de Groot R
- Subjects
- Adolescent, CD4 Lymphocyte Count, Child, Child, Preschool, Cohort Studies, Female, HIV Infections virology, Humans, Male, Prospective Studies, Time Factors, Treatment Outcome, Antiretroviral Therapy, Highly Active methods, HIV Infections drug therapy, HIV Infections immunology, HIV-1 drug effects, RNA, Viral blood
- Abstract
We report the data from a long-term study of 31 human immunodeficiency virus type 1 (HIV-1)-infected children who were treated with highly active antiretroviral therapy. A high proportion of the children had undetectable HIV-1 RNA levels. CD4+ T cell counts recovered and remained stable. Adverse events were observed frequently but were mostly mild.
- Published
- 2005
- Full Text
- View/download PDF
5. Abnormalities in the T and NK lymphocyte phenotype in patients with Nijmegen breakage syndrome.
- Author
-
Michałkiewicz J, Barth C, Chrzanowska K, Gregorek H, Syczewska M, Weemaes CM, Madaliński K, and Stachowski J
- Subjects
- Adolescent, Antigens, CD immunology, CD3 Complex analysis, CD4 Antigens analysis, CD4-Positive T-Lymphocytes immunology, CD56 Antigen analysis, CD8 Antigens analysis, CD8-Positive T-Lymphocytes immunology, Case-Control Studies, Child, Child, Preschool, Female, Flow Cytometry, Humans, Immunologic Memory, Immunophenotyping, Infant, Killer Cells, Natural immunology, Leukocyte Common Antigens analysis, Lymphocyte Count, Male, Receptors, Antigen, T-Cell, alpha-beta analysis, Receptors, Antigen, T-Cell, gamma-delta analysis, Antigens, CD analysis, Chromosome Breakage, Immune System Diseases immunology, T-Lymphocyte Subsets immunology
- Abstract
Nijmegen breakage syndrome (NBS) is a rare autosomal recessive disorder characterized by spontaneous chromosomal instability with predisposition to immunodeficiency and cancer. In order to assess the cellular basis of the compromised immune response of NBS patients, the distribution of functionally distinct lymphocyte subsets in peripheral blood was evaluated by means of double-colour flow cytometry. The study involved the 36 lymphopenic patients with a total lymphocyte count < or =1500 microl (group A) and seven patients (group B) having the absolute lymphocyte count comparable with the age-matched controls (> or =3000 microl). Regardless of the total lymphocyte count the NBS patients showed: (1) profound deficiency of CD4+ and CD3/CD8+ T cell subsets and up to fourfold increase in natural killer (NK) cells, almost lack of naive CD4+ T cells expressing CD45RA isoform, unchanged percentage of naive CD8+ cell subset (CD8/CD45RA+) but bearing the CD8 receptor of low density (CD8low); (2) normal expression of CD45RA isoform in the CD56+ lymphocyte subset, profound decrease in alpha beta but up to threefold increase in gamma delta-T cell-receptor (TCR)-positive T cells; (3) shift towards the memory phenotype in both CD4+ and CD8+ lymphocyte subpopulations expressing CD45RO isoform (over-expression of CD45RO in terms of both the fluorescence intensity for CD45RO isoform and the number of positive cells); and (4) an increase in fluorescence intensity for the CD45RA isoform in NK cells population. These results indicate either a failure in T cell regeneration in the thymic pathway (deficiency of naive CD4+ cells) and/or more dominant contribution of non-thymic pathways in lymphocyte renewal reflected by an increase in the population of CD4+ and CD8+ memory cells, gamma delta-TCR positive T as well as NK cell subsets.
- Published
- 2003
- Full Text
- View/download PDF
6. Increased expression of interleukin-13 but not interleukin-4 in CD4+ cells from patients with the hyper-IgE syndrome.
- Author
-
Gudmundsson KO, Sigurjonsson OE, Gudmundsson S, Goldblatt D, Weemaes CM, and Haraldsson A
- Subjects
- CD4-Positive T-Lymphocytes cytology, Cells, Cultured, Female, Humans, Job Syndrome blood, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear immunology, Male, CD4-Positive T-Lymphocytes immunology, Interleukin-13 biosynthesis, Interleukin-4 biosynthesis, Job Syndrome immunology
- Abstract
Hyper IgE syndrome (HIES) is a rare immunodeficiency disorder characterized mainly by high levels of polyclonal IgE in serum and recurrent staphylococcal abscesses of the skin and lungs. The raised IgE levels have led researchers to study the synthesis of cytokines that regulate switching of immunoglobulin production towards IgE such as interleukin-4 (IL-4), IL-12 and interferon-gamma (IFN)-gamma. However, the role of IL-13 in the disease pathogenesis has not been investigated extensively. In this study, we investigated intracellular expression of IL-4 and IL-13 in mononuclear cells and CD4+ cells isolated from patients with HIES and healthy controls. Cells were stained intracellularly with antibodies directed against IL-4 and IL-13 and analysed by flow cytometry before and after activation with PMA and calcium ionophore. The mean proportion of resting or activated IL-4 and IL-13 expressing mononuclear cells were comparable in the two groups as well as the proportion of IL-4 expressing CD4+ cells. In contrast, the mean proportion of IL-13 expressing CD4+ cells was increased significantly in patients with HIES in both the resting and the activated state compared to healthy controls. We conclude that increased expression of IL-13 in CD4+ cells from patients with HIES could account, at least partly, for raised IgE levels in those individuals.
- Published
- 2002
- Full Text
- View/download PDF
7. Results of 2 years of treatment with protease-inhibitor--containing antiretroviral therapy in dutch children infected with human immunodeficiency virus type 1.
- Author
-
van Rossum AM, Geelen SP, Hartwig NG, Wolfs TF, Weemaes CM, Scherpbier HJ, van Lochem EG, Hop WC, Schutten M, Osterhaus AD, Burger DM, and de Groot R
- Subjects
- Adolescent, CD4 Lymphocyte Count, Child, Child, Preschool, Drug Therapy, Combination, Female, HIV Infections epidemiology, HIV Protease Inhibitors adverse effects, HIV-1 drug effects, Humans, Indinavir adverse effects, Indinavir therapeutic use, Infant, Lamivudine adverse effects, Lamivudine therapeutic use, Male, Nelfinavir adverse effects, Nelfinavir therapeutic use, Netherlands epidemiology, Prospective Studies, Reverse Transcriptase Inhibitors adverse effects, Treatment Outcome, Zidovudine adverse effects, Zidovudine therapeutic use, HIV Infections drug therapy, HIV Protease Inhibitors therapeutic use, Reverse Transcriptase Inhibitors therapeutic use
- Abstract
Clinical, virologic, and immunologic responses to treatment that contained either indinavir or nelfinavir (both regimens included zidovudine and lamivudine) were determined in 32 children infected with human immunodeficiency virus type 1 (HIV-1) who participated for >/= 96 weeks in a prospective, open, uncontrolled multicenter trial. The pharmacokinetics of indinavir and of nelfinavir were determined and showed large interindividual differences. After 96 weeks of therapy, 69% and 50% of the patients had an HIV-1 RNA load that was below the HIV assays' detection limits of 500 and 40 copies/mL, respectively. Virologic failure was associated with poor compliance and younger age (independent of baseline virus load and receipt of pretreatment). Relative CD4 cell counts increased significantly in relation to the median of the age-specific reference value, from a median of 44% at baseline to 94% after 96 weeks. In a high percentage of the children, clinical, virologic, and immunologic response rates to combination therapy were optimal during the initial 2 years of therapy.
- Published
- 2002
- Full Text
- View/download PDF
8. Skin manifestations in congenital deficiency of leucocyte-adherence glycoproteins (CDLG).
- Author
-
van de Kerkhof PC and Weemaes CM
- Subjects
- Adult, Female, Gangrene, Humans, Male, Skin pathology, Skin Ulcer pathology, Leukocyte-Adhesion Deficiency Syndrome, Metabolism, Inborn Errors pathology, Skin Ulcer metabolism
- Abstract
In congenital deficiency of leucocyte-adherence glycoproteins (CDLG) there is an immunodeficiency with impaired leucocyte function and cutaneous and extracutaneous infections occur. In more than 30% of cases the condition has a fatal course. We report the skin manifestations of three siblings with CDLG in which areas of skin necrosis occurred that resembled pyoderma gangrenosum.
- Published
- 1990
- Full Text
- View/download PDF
9. Nephelometry of the kappa/lambda light-chain ratio in serum of normal and diseased children.
- Author
-
Renckens AL, Jansen MJ, van Munster PJ, Weemaes CM, and Bakkeren JA
- Subjects
- Adolescent, Arthritis, Juvenile immunology, Child, Child, Preschool, Dysgammaglobulinemia immunology, Fetal Blood immunology, Humans, IgA Deficiency, Immunoglobulin G analysis, Infant, Leukemia, Lymphoid immunology, Nephelometry and Turbidimetry, Reference Values, Immunoglobulin kappa-Chains analysis, Immunoglobulin lambda-Chains analysis
- Abstract
We determined, immunonephelometrically, the ratio between the two types of light chains of immunoglobulins, kappa and lambda, in serum of 94 children, ages 0.4 to 14 years, with no manifest immunological disorders. Children with an abnormal protein pattern by immunoelectrophoresis show other values for this ratio than do children in this reference group. We also determined the ratios for children with IgA deficiency (I), juvenile rheumatic arthritis (II), and acute lymphoblastic leukemia (III). Children with I show the same kappa/lambda ratios as for the children in the reference group. Children with II also show the same mean kappa/lambda ratios, but a significantly wider range of ratios. In children with III, the ratio during chemotherapy is slightly depressed, significantly lower than after cessation of therapy. All groups--healthy children and patients--show an increase in kappa-chain-bearing immunoglobulins with age, but the concentration of lambda-chain-bearing immunoglobulins remains relatively constant.
- Published
- 1986
10. Antibody responses in vivo in chromosome instability syndromes with immunodeficiency.
- Author
-
Weemaes CM, The TH, van Munster PJ, and Bakkeren JA
- Subjects
- Adolescent, Child, Child, Preschool, Chromosome Disorders, Diphtheria Toxoid immunology, Female, Hemocyanins pharmacology, Humans, Immunoglobulin A biosynthesis, Immunoglobulin G biosynthesis, Immunoglobulin M biosynthesis, Immunologic Memory, Male, Poliovirus Vaccine, Inactivated immunology, Tetanus Toxoid immunology, Ataxia Telangiectasia immunology, Bloom Syndrome immunology, Chromosome Aberrations immunology
- Abstract
The primary IgG, IgM and IgA antibody responses to Helix pommatia haemocyanin (HPH) were defective in patients with ataxia telangiectasia (AT) and Nijmegen breakage syndrome (NBS). The results in patients with Bloom's syndrome (BS) were heterogeneous, but all showed abnormal kinetics of the IgG response. The secondary response to diphtheria, tetanus and polio vaccine was normal in patients with AT and BS, but disturbed in the patients with NBS. The abnormalities of antibody response of AT and NBS are similar, although more profound in NBS; BS is different.
- Published
- 1984
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.