1. Production of galactinol from sucrose by plant enzymes.
- Author
-
Wakiuchi N, Shiomi R, and Tamaki H
- Subjects
- Chromatography, High Pressure Liquid, Galactosyltransferases antagonists & inhibitors, Galactosyltransferases metabolism, Glucosyltransferases metabolism, Magnetic Resonance Spectroscopy, Cucumis sativus enzymology, Disaccharides biosynthesis, Ipomoea batatas enzymology, Sucrose metabolism
- Abstract
Galactinol, 1-O-(alpha-D-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. UDP-Glc was prepared with sucrose and UDP using sucrose synthase partially purified from sweet potato roots. Then, the UDP-Glc was converted to UDP-Gal using yeast UDP-Gal 4-epimerase from a commercial source. Finally, galactinol was produced from the UDP-Gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. The product was identified as galactinol by the retention times of HPLC, alpha-galactosidase digestion, and NMR spectrometry.
- Published
- 2003
- Full Text
- View/download PDF