1. Suppression of rat epididymal sperm immunogenicity by a seminal vesicle secretory protein and transglutaminase both in vivo and in vitro.
- Author
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Peluso G, Porta R, Esposito C, Tufano MA, Toraldo R, Vuotto ML, Ravagnan G, and Metafora S
- Subjects
- Animals, Antigen Presentation, Epithelium metabolism, Female, Histocompatibility Antigens Class II metabolism, Macrophage Activation drug effects, Macrophages, Peritoneal drug effects, Macrophages, Peritoneal physiology, Male, Phagocytosis, Rats, Rats, Inbred BN, Rats, Wistar, Seminal Plasma Proteins, Superoxides metabolism, Epididymis cytology, Prostatic Secretory Proteins, Proteins pharmacology, Seminal Vesicles metabolism, Spermatozoa immunology, Transglutaminases pharmacology
- Abstract
The pretreatment of epididymal spermatozoa with SV-IV, one of the major secretory protein produced by the epithelium of adult rat seminal vesicles, was found to markedly decrease their ability to induce in vivo peritoneal macrophage activation, measured as class II major histocompatibility complex surface antigen expression, superoxide anion production, phagocytic activity, and antigen presentation. In addition, the treatment of spermatozoa with SV-IV produced a significant decrease of their immunogenicity evaluated in vitro by [3H]thymidine incorporation in splenocyte/spermatozoon co-culture. The concurrent presence of SV-IV and transglutaminase, an enzyme secreted in large amounts from the rat anterior prostate, amplified these phenomena. The suppression of the epididymal sperm immunogenicity is suggested to be of crucial importance for the prevention of the immune response to the sperm introduced in the immunocompetent female genital tract during coitus.
- Published
- 1994
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