1. AIDS-antiviral sulfolipids from cyanobacteria (blue-green algae).
- Author
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Gustafson KR, Cardellina JH 2nd, Fuller RW, Weislow OS, Kiser RF, Snader KM, Patterson GM, and Boyd MR
- Subjects
- Chemical Phenomena, Chemistry, HIV Core Protein p24, Lipids isolation & purification, Microbial Sensitivity Tests methods, Retroviridae Proteins analysis, Tetrazolium Salts, Antiviral Agents isolation & purification, Cyanobacteria analysis, HIV drug effects, Lipids pharmacology
- Abstract
A recently developed tetrazolium-based microculture assay was used to screen extracts of cultured cyanobacteria (blue-green algae) for inhibition of the cytopathic effects of the human immunodeficiency virus (HIV-1), which is implicated as a causative agent of AIDS. A number of extracts were found to be remarkably active against the AIDS virus. A new class of HIV-1-inhibitory compounds, the sulfonic acid-containing glycolipids, was discovered through the use of the microculture assay to guide the fractionation and purification process. The pure compounds were active against HIV-1 in cultured human lymphoblastoid CEM, MT-2, LDV-7, and C3-44 cell lines in the tetrazolium assay as well as in p24 viral protein and syncytium formation assays.
- Published
- 1989
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