1. MD-2 expression is not required for cell surface targeting of Toll-like receptor 4 (TLR4).
- Author
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Visintin A, Halmen KA, Khan N, Monks BG, Golenbock DT, and Lien E
- Subjects
- Animals, Cell Line, Humans, Lipopolysaccharides pharmacology, Lymphocyte Antigen 96 deficiency, Lymphocyte Antigen 96 immunology, Mice, Mice, Knockout, Toll-Like Receptor 4 deficiency, Toll-Like Receptor 4 immunology, Gene Expression Regulation drug effects, Gene Expression Regulation genetics, Gene Expression Regulation immunology, Lymphocyte Antigen 96 biosynthesis, Toll-Like Receptor 4 biosynthesis, Toll-Like Receptor 4 metabolism
- Abstract
The cell surface receptor complex formed by TLR4 and myeloid differentiation 2 (MD-2) is engaged when cells are exposed to LPS. Recent studies suggested that surface localization of functional mouse TLR4 (mTLR4) depends on the simultaneous expression of MD-2. As we did not observe a similar requirement, we conducted a comparative study of human TLR4 and mTLR4 surface expression in immune cells derived from the MD-2 knockout mouse and LPS-responsive cell lines and in cells that ectopically express TLR4. Our results indicate that in the human and mouse models, neither TLR4 function nor TLR4 surface targeting requires MD-2 coexpression. Accordingly, we report on one human cell line, which constitutively expresses functional TLR4 on the cell surface in the absence of MD-2 expression.
- Published
- 2006
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