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The Doppelgänger phenomenon refers to the experience of a direct encounter with one’s self, characteriswed by: (i) the perception of a figure with one’s own identical physical features; or (ii) the apprehension that the perceived figure shares the same personality and identity. The Doppelgänger does not only look like the same person, it is his/her double. The perceptual element is usually a hallucination, although occasionally a false perception of an actual figure may be involved. This phenomenon has been described in individuals suffering from overwhelming fear, severe anxiety or intoxication, epilepsy, as well as in the sleep-wakefulness transition. It has also been reported in major psychoses. The fear of imminent death often precedes the Doppelgänger experience. This report presents the case of a 30-year-old man, Mr. Y, who was stabbed to death by Mr. X, his “double”. The aggressor and his victim, although not related, were truly doubles; remarkably, they shared the same name and surname, age, professional activity and place of work. Moreover, they attended the same sports center but barely knew each other. The forensic psychiatric evaluations in Mr. X, subsequent to the crime committed, were suggestive of a psychotic condition. This case is unique in the scientific literature. In the most serious psychotic forms, the issue of the “double” calls into question not only the dissociative processes involved in the etiopathogenesis of the disorder, but also bio-psycho-social elements, as well as personal data in this case, which made the victim and the aggressor “identical”. In the context of psychopathological functioning, the delusional mood (Wahnstimmung) that precedes the development of delirium is a sort of gateway to an impending psychotic illness, involving delusional awareness or mood (atmosphere). In psychosis, splitting is the main issue and this influence is seen as an evil, foreign, apocalyptic and unknown side no longer recognised as belonging to the self even in a physical sense. In such a situation, it is felt that the only way to survive is by suppressing one’s double as a defense against disorganisation of the self.

Several decomposed body parts were received for examination by the Forensic Anthropology section of the Medico-legal Institute of João Pessoa, Paraíba, Brazil. The portions of the lower and upper limbs, ribs, vertebrae, and a skull were thoroughly examined. The biological profile indicated a male individual with an estimated age range between 23 and 57 years and a mean age of 35.2 years (SD = 9.4; phase IV, Suchey-Brooks). The skeleton showed injuries caused by sharp force and sharp-blunt force trauma that affected all body segments. Macroscopically, the lesions are mainly in the diaphyseal segments of the long bones, sacrum, pelvis, mandible, maxilla, scapulae, sternum, vertebrae, the distal epiphysis of the left fibula, and the distal epiphysis of the left tibia displayed characteristics compatible with injuries produced perimortem. It was not possible to determine the cause of death. DNA analysis resulted in a positive identification. Because of common difficulties faced in forensic practice, it is often not possible for forensic anthropologists to go to the crime scene, X-ray or body scanners are frequently unavailable, and the victim’s medical and/or dental records are sometimes absent. These difficulties make identification ultimately depend on genetic analysis, which is more time-consuming than other identification methods. Despite this, bone fragment examination in dismemberment cases is a complex task. Forensic Anthropology can shed light on cases involving the identification of dismembered remains, which are challenging because of the number of traumatic injuries, as well as different injury patterns, on bones.

Wound age estimation is a crucial and challenging problem in forensic pathology. Although mRNA is the most commonly used indicator for wound age estimation, screening criteria are lacking. In the present study, the feasibility of screening criteria using mRNA to determine injury time based on the adenylate-uridylate-rich element (ARE) structure and gene ontology (GO) categories were evaluated. A total of 78 Sprague-Dawley male rats were contused and sampled at 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, and 48 h after inflicting injury. The candidate mRNAs were classified based on with or without ARE structure and GO category function. The mRNA expression levels were detected using qRT-PCR. In addition, the standard deviation (STD), mean deviation (MD), relative average deviation (d%), and coefficient of variation (CV) were calculated based on mRNA expression levels. The CV score (CVs) and the CV of CV (CV’CV) were calculated to measure heterogeneity. Finally, based on classic principles, the accuracy of combination of candidate mRNAs was assessed using discriminant analysis to construct a multivariate model for inferring wound age. The results of homogeneity evaluation of each group based on CVs were consistent with the MD, STD, d%, and CV results, indicating the credibility of the evaluation results based on CVs. The candidate mRNAs without ARE structure and classified as cellular component (CC) GO category (ARE–CC) had the highest CVs, showing the mRNAs with these characteristics are the most homogenous mRNAs and best suited for wound age estimation. The highest accuracy was 91.0% when the mRNAs without ARE structure were used to infer the wound age based on the discrimination model. The accuracy of mRNAs classified into CC or multiple function (MF) GO category was higher than mRNAs in the biological process (BP) category. In all subgroups, the accuracy of the composite identification model of mRNA composition without ARE structure and classified as CC was higher than other subgroups. The mRNAs without ARE structure and belonging to the CC GO category were more homogenous, showed higher accuracy for estimating wound age, and were appropriate for rat skeletal muscle wound age estimation.

Many studies have examined the genetic contribution to suicide. However, data on suicide in the Italian population are scarce. We therefore aimed to address this gap by investigating a cohort of 111 Italians for whom a verdict of suicide had been declared in court in Florence, Italy between 2007 and 2017. This cohort included 86 men and 25 women. DNA samples were obtained from tissues or blood, and 22 genes from multiple neurobiological pathways previously shown to be associated with the pathogenesis of suicide were analysed. Next-generation sequencing was used to compare these gene sequences with those from a large, normal population. In this study, we identified 19 gene variants that were present at significantly lower frequencies in our Italian cohort than in the general population. In addition, four missense mutations were identified in four different genes: Monoamine Oxidase A (MAOA), 5-Hydroxytryptamine Receptor 2 A (HTR2A), Sodium Voltage-Gated Channel Alpha Subunit 8 (SCN8A), and Nitric Oxide Synthase 3 (NOS3). Our study identified several potential genetic links with suicide in a cohort of Italians and supports a relationship between specific genetic variants and suicidal behaviour in this population. Key pointsThis study shows a genetic analysis of suicide.This study examines a cohort of 111 Italians for whom a verdict of suicide had been declared in court in Florence, Italy between 2007 and 2017. DNA samples were obtained from tissues or blood, and 22 genes from multiple neurobiological pathways were investigated.The study supports a relationship between specific genetic variants and suicidal behaviour.

In forensic medical examinations, estimating the postmortem interval (PMI) is an important factor. Methamphetamine (MA) is a synthetic stimulant that is commonly abused, and estimation of the PMI after MA abuse has become one of the main tasks in forensic investigation. Microorganisms play a vital role in carrion decomposition. Analysing the bacterial succession patterns can be used as a forensic tool to estimate the PMI. The present study aimed to analyse bacterial succession changes during the decomposition of MA to estimate the PMI. We analysed bacterial communities in rabbits treated with three different concentrations of MA (0, 22.5, and 90 mg/kg) under the natural conditions of 20 °C and 70% humidity by sequencing 16S rRNA gene amplicons using the Illumina MiSeq system. We obtained 2 774 209 high-quality sequences and 2937 operational taxonomic units (OTUs). The relative abundances of the bacterial communities varied markedly in response to different MA concentrations. Interestingly, in response to the different concentrations of MA, Bacteroidetes became disparate in the rectum in the late PMI. Increased numbers of bacterial taxa were identified in the rectum and buccal cavity samples, except at the highest concentration of MA in the rectum samples when PMI was 0–h, than were present in live rabbits. Meanwhile, the PMI correlated significantly with bacterial succession at different taxonomic levels. Our results suggested that bacterial community succession could be used as a “microbial clock” to estimate the PMI in cases of MA-related death; however, further study is required to gain a deeper understanding of this concept.KeypointsAs a forensic tool, microbiome succession patterns have great potential for PMI estimation.At different taxonomic levels, the variation in the succession of microorganisms’ under three different concentrations of MA during the processes of decomposition is significantly related to PMI for estimating MA abuse.These findings provide a new idea for the estimation of PMI of MA abused by changes in microorganisms.

Cyanoacrylate fluorescent staining has become a common method for developing latent fingermarks on light-coloured or white objects. The method requires two steps and skilled operation, and the handling process has the risk of damaging the ridge details or even biological samples. To take full advantage of the high-sensitivity fluorescence, recent research efforts on fluorescent cyanoacrylate have aimed to avoid rinsing after staining, add fluorescence to the cyanoacrylate, increase the fluorescence intensity and broaden the fluorescence spectral range. PolyCyano UV is a novel product that can be used to overcome the disadvantages associated with progressing to one-step fuming to directly develop fingermarks. To explore the optimal development conditions and application effects of PolyCyano UV, thermogravimetric differential thermal analysis, fluorescence spectroscopy and control variable analysis are used to determine and analyse the best conditions for using the reagent, including temperature, fluorescence excitation band, relative humidity and fuming concentration. The temperature range of the one-step fuming development method is 212.14 °C–275.16 °C, the wavelength range of the excitation light source is 235–580 nm, the relative humidity is 60%–80%, the concentration of the fume is 1–3 g/m3 and the specific value is related to the surface properties of the object and the aged time of the fingermarks. Additionally, fluorescence spectroscopy shows that the excitation wavelength range of the light source is 235–580 nm. Based on the best fuming condition, many common objects were selected to proceed to the application experiments, which allowed for a comparison with the traditional visualisation method, namely the Cyanobloom reagent + dyeing two-step development method. The comparison showed that latent fingermarks on most non-porous smooth surfaces, especially on light-coloured or white objects, were developed successfully. In addition, a comparative study with the conventional cyanoacrylate glue–fluorescent staining experiments showed that the effect of dyeing afterwards with Rhodamine 6 G is better than the one-step method. The effect of dyeing with BBD is basically the same as that of the one-step method. Key points:One-step fluorescent cyanoacrylate fuming can be a promising alternative for fingermarks developing based on PolyCyano UV in forensic science.The optimum application conditions of PolyCyano UV will provide guidance for fingermark examiners.The one-step fuming method based on PolyCyano UV has many advantages over the secondary dyeing method.

In 2017, the Republic of Kazakhstan began the phased transition of its alphabet from Cyrillic to Latin script. This transition has presented significant challenges to Kazakhstani document examiners, who have yet to develop appropriate methodologies for the analysis of handwriting samples written in the Kazakh language using Latin letters. This study aims to identify distinguishing macro and micro features of letters within Kazakh writing samples produced using the Latin alphabet and determine their frequencies of occurrence and discriminating power indices. Micro features were examined using the four most frequently appearing letters: “a”, “y”, “e” and “n”. A comparative analysis of tested Latin letters with those of a similar configuration in Cyrillic demonstrated differences in the number of distinguishing features, as well as in the frequency of occurrence and discriminating power indices of similar features. These results show that separate statistical bases should be used for Latin and Cyrillic letters when analysing handwriting samples based on the frequencies of occurrence of micro and macro writing features.

Since the start of the COVID-19 pandemic, there has been an urgent need to produce accurate and sensitive tests. However, there have been instances where a positive nucleic acid test turns negative after treatment, and then positive again. This case report describes such an instance from the tropical region of Hainan, China. The patient was a 61-year-old female who went to Hainan on vacation from Wuhan during the COVID-19 pandemic in 2020. Symptoms appeared 9 d after arriving in Hainan, and it was confirmed that the nucleic acid test was positive after 4 repeats. Her condition declined rapidly, her heart stopped beating, and she was admitted in a coma to the ICU. After treatment, the SARS-CoV-2 virus nucleic acid test of several nasopharyngeal swabs were negative, and tests on whole blood, anal swabs, and urine were also negative. Later, however, nucleic acid tests on a lower respiratory tract sputum swab and lower respiratory tract lavage fluid were positive. An autopsy examination was carried out 12 h after her death, and multi-organ secretions were extracted for nucleic acid testing. The SARS-CoV-2 virus nucleic acid was only detected in the swabs from the end of the bronchus, which was confirmed by the visualization of the coronavirus by electron microscopy. Autopsy confirmed that the damage was mainly concentrated in the lungs and immune organs and tissues throughout the body. Epidemiology indicated that none of the people she came into contact with after arriving in Hainan, including close contacts, were infected. This is in sharp contrast to the highly contagious virus in Wuhan in the temperate zone during the same period. This case report indicates: (1) The high temperatures in tropical areas may have an impact on the spread and harm of COVID-19, and (2) The reason why nucleic acid testing for COVID-19 was initially negative and then positive after treatment may be related to the survival of the SARS-CoV-2 virus in deep lung tissues.

Forensic imaging is a non-invasive examination process during the forensic investigation. It is mainly used in forensic pathology as an adjunct to the traditional autopsy. In the past two decades, forensic imaging has been vigorously developed by forensic experts from computed tomography (CT) to multiple augmented techniques through CT and magnetic resonance imaging (MRI). The application field of forensic imaging has also been broadened as its advantages are recognised by more forensic practitioners. In addition to the forensic pathology, this technique has been used in other forensic disciplines, including forensic anthropology, forensic odontology, forensic ballistics and wildlife forensics, etc. This article reviews the development of forensic imaging as the practice and research development in different forensic disciplines based on the relevant literature analysis.

Disaster victim identification issues are especially critical and urgent after a large-scale disaster. The aim of this study was to suggest an automatic detection of natural teeth and dental treatment patterns based on dental panoramic radiographs (DPRs) using deep learning to promote its applicability as human identifiers. A total of 1 638 DPRs, of which the chronological age ranged from 20 to 49 years old, were collected from January 2000 to November 2020. This dataset consisted of natural teeth, prostheses, teeth with root canal treatment, and implants. The detection of natural teeth and dental treatment patterns including the identification of teeth number was done with a pre-trained object detection network which was a convolutional neural network modified by EfficientDet-D3. The objective metrics for the average precision were 99.1% for natural teeth, 80.6% for prostheses, 81.2% for treated root canals, and 96.8% for implants, respectively. The values for the average recall were 99.6%, 84.3%, 89.2%, and 98.1%, in the same order, respectively. This study showed outstanding performance of convolutional neural network using dental panoramic radiographs in automatically identifying teeth number and detecting natural teeth, prostheses, treated root canals, and implants.

This article describes the forensic odontological analysis of the events of the 2015 Nepal earthquake. It identifies the problems encountered in the aftermath, lessons learned, and prospective future advances aimed at reducing the subjectivity in disaster victim identification (DVI). During a crisis, dental practitioners, particularly forensic odontologists, can make a substantial contribution to DVI, as highlighted in this article. It also promotes best practices in forensic dentistry that may be used by anyone in situations with few resources or people to deal with comparable scenarios.

Anthropologists are often the custodians of long-term unidentified human remains though their positions as curators of university or museum skeletal collections. Various factors decrease the solvability of these legacy cases including the passage of time, the loss of provenience for specific cases, and lack of documentation or case records. While anthropologists can contribute important information toward identification, it is often necessary to explore novel and cross-disciplinary strategies to resolve difficult cold cases.In long cold cases, the postmortem interval, in particular, may be difficult to estimate leading to further challenges in achieving identification. Modern advances in radiocarbon bomb pulse dating, isotope analysis, and actualistic studies have contributed to positive identification of unidentified human remains in some legacy cases, but may not be available to all forensic practitioners and law enforcement from resource-poor agencies. Pooling resources, as well as collaborating with professionals outside of forensic anthropology, is a useful strategy to pursue when anthropological methods are exhausted.The case study presented here demonstrates a collaborative approach between forensic anthropologists, forensic genetic genealogists, and law enforcement in a century-old homicide. The dismembered and mummified parts of a male body were recovered in a remote cave in 1979 and again in 1991. Despite forensic anthropologists creating and updating the biological profile over the decades from recovery to present, no identification was made until the application of forensic genetic genealogy (FGG) to the case in 2019. New interpretations of bone microstructure and trauma analysis are presented for the case, alongside the historical documentation and “proof of life” evidence used by the genealogy team. A review of the FGG methods underscores the challenges in this case (e.g. significant endogamy, multiple aliases used by the victim) and the steps taken toward resolution. Ultimately, a combined anthropology and genealogy approach resulted in a confirmed identity for a man who was murdered in 1916.Key pointsForensic scientists should leverage a collaborative, interdisciplinary approach toward human identification.When combined with forensic anthropology methods, forensic genetic genealogy is a valuable tool linking biological and cultural-historical aspects of identity.Forensic anthropologists should review challenging cases in their labs as new methods are introduced and new resources become available.

Forensic anthropologists are often responsible for the management of long-term unidentified individuals. Others have contextualised these decedents—many of whom likely belonged to socially, politically, and/or economically marginalised groups in life—as part of a larger identification crisis in the United States. However, there has been little discussion surrounding how this humanitarian crisis has manifested in academic institutions, where anthropologists often provide medicolegal consultation and act as long-term stewards of the unidentified. The Identification & Repatriation Initiative was created at the Forensic Anthropology Center at Texas State University (FACTS) to recognise and investigate unidentified human remains in long-term storage. Our paper outlines common challenges that were encountered during our initial reassessment of unidentified cases at FACTS, emphasising the detrimental impacts of inconsistent procedures, loss of context, and case fatigue. It is likely that other academic institutions face similar challenges, and by highlighting these issues we hope to help initiate a larger conversation concerning ethical stewardship of human remains in these settings. By incorporating humanitarian perspectives into forensic casework, anthropologists in academia can better advocate for the long-term unidentified.Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2022.2035063 .

Violence against the neck can result in a range of macromorphological and micromorphological findings. However, the forensic relevance of the carotid sinus in cases of violence against the neck remains controversial. In this follow-up study of 22 cases of suicidal and accidental strangulations, carotid bifurcations were examined histologically for morphological changes implying direct trauma, including haemorrhage and immunohistochemical expression of heat-shock proteins 27, 60, and 70 and aquaporin-3. These cases were compared with a control group (82 cases) without neck compression or head trauma and with variable causes of death. No relevant histopathological findings implying direct trauma of the carotid bifurcation were found. No cases showed positive aquaporin-3 staining and only five cases showed positive heat-shock protein-27 staining, all of which were hangings. Without massive trauma of the carotid bifurcation, histological alterations cannot be expected. Without signs of rapid death, findings of acute circulatory failure, macromorphological and micromorphological findings of neck compression, and reliable markers indicating relevant impact on the carotid bifurcation the diagnosis of a lethal reflex cannot be verified.Key pointsAmong 22 cases of strangulation causing death, there were 16 cases of hanging and 6 cases of ligature strangulationFew cases showed small haemorrhages located predominantly in the surrounding fat and connective tissues; however, the haemorrhages did not have any effects on the tissuesNeck compression had minimal effects on heat shock protein 27 expression in carotid artery tissueAquaporin-3 staining suggested it is not a useful marker for relevant neck pressure, or that there had not been any relevant neck impactOur findings suggested no direct evidence for reflex cardiac death resulting from a brief force against the neck

Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity cases with genetic inconsistencies, a total of 63 parentage confirmed paternity cases contained at least one mismatched locus have been collected. The mutations were subsequently evaluated using Verogen’s MPS ForenSeqTM DNA Signature Kit and a microsatellite instability (MSI) detection kit. The result showed 98.41% (62/63) of the cases had no additional autosomal mutations even when the number of A-STRs increased to 27. As for the sex chromosomes, about 11.11% (7/63) of the cases exhibited either X-STR or Y-STR mutations. D2S1338, FGA and Penta E were the most frequent altered STRs, which suggested they might be the mutation hotspots. In addition, a male with sex chromosome abnormality was observed accidently, whose genotype might be 47, XXY, rather than MSI. Nearly 56.90% of the STR loci possessed isoalleles, which might result in higher STR polymorphisms. No Mendelian incompatibility was detected among the SNP markers, which indicated that SNP was a more reliable genetic marker in the genetic-inconsistent paternity cases.Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.2009631 .

Toolmarks, particularly those found on bulky, inaccessible or immovable items, can be recovered by casting. To allow for subsequent comparative examinations, the casting material, typically polysiloxanes or silicones, must be able to capture and preserve fine details within a toolmark accurately. To study the stability of such details after exposure to heat, toolmark casts were heated at either 60 °C for 2 h, or 90 °C for 1 h. These casts were subsequently compared to casts that had not been exposed to heat, using traditional optical comparison microscopy, as well as virtual comparison microscopy. Digitised toolmark signatures were also extracted from the casts and compared pairwise to obtain quantitative similarity scores based on cross-correlation, consecutive matching striae and Mann-Whitney U-statistic. Our results show that the fine surface details captured on all four commercial toolmark casting materials tested herein remained stable after exposure to heat. This study shows that the above heating protocols are viable viral inactivation methods for toolmark casts that are potentially contaminated with human coronaviruses, such as SARS-CoV-2. Our findings also apply to other scenarios, such as for casts that were left in a vehicle parked under the sun.

Disaster victim identification (DVI) entails a protracted process of evidence collection and data matching to reconcile physical remains with victim identity. Technology is critical to DVI by enabling the linkage of physical evidence to information. However, labelling physical remains and collecting data at the scene are dominated by low-technology paper-based practices. We ask, how can technology help us tag and track the victims of disaster? Our response to this question has two parts. First, we conducted a human–computer interaction led investigation into the systematic factors impacting DVI tagging and tracking processes. Through interviews with Australian DVI practitioners, we explored how technologies to improve linkage might fit with prevailing work practices and preferences; practical and social considerations; and existing systems and processes. We focused on tagging and tracking activities throughout the DVI process. Using insights from these interviews and relevant literature, we identified four critical themes: protocols and training; stress and stressors; the plurality of information capture and management systems; and practicalities and constraints. Second, these findings were iteratively discussed by the authors, who have combined expertise across electronics, data science, cybersecurity, human–computer interaction and forensic pathology. We applied the themes identified in the first part of the investigation to critically review technologies that could support DVI practitioners by enhancing DVI processes that link physical evidence to information. This resulted in an overview of candidate technologies matched with consideration of their key attributes. This study recognises the importance of considering human factors that can affect technology adoption into existing practices. Consequently, we provide a searchable table (as Supplementary information) that relates technologies to the key considerations and attributes relevant to DVI practice, for readers to apply to their own context. While this research directly contributes to DVI, it also has applications to other domains in which a physical/digital linkage is required, and particularly within high stress environments with little room for error.Key Points:Disaster victim identification (DVI) processes require us to link physical evidence and digital information. While technology could improve this linkage, experience shows that technological “solutions” are not always adopted in practice.Our study of the practices, preferences and contexts of Australian DVI practitioners suggests 10 critical considerations for these technologies.We review and evaluate 44 candidate technologies against these considerations and highlight the role of human factors in adoption.

As of 2015, 204 cases of missing and murdered Indigenous women and girls (MMIWG) remained unsolved in Canada, making it a major concern for Canadian Indigenous communities, who are still pressing for the resolution of these cases. In forensic anthropology, the assessment of population affinity can be useful to help identify victims. Population affinity, previously referred to as ancestry, is evaluated based on morphological analyses, which examine the size and shape of skeletal features, and metric analyses, which utilise skeletal measurements. However, morphological analyses strongly depend on an anthropologist’s experience with human variation, which makes the analyses particularly challenging to reproduce and standardise. The purpose of this study is to improve the rigour of morphological analyses by using 3D technology to quantify relevant cranial nonmetric population affinity traits. As there is currently little morphological data available for the Canadian Indigenous population, this research aims to develop a new technique that could aid in the identification of MMIWG. The study comprised a total of 87 adult female crania, including 24 of Canadian Inuit origin, 50 of European descent and 13 of African descent. The samples were imaged using photogrammetry, then analysed using a 3D shape analysis in 3DS Max. Results show that this method is satisfactory in correctly evaluating population affinity with an accuracy of 87.36% (jackknifed: 80.46%) and an average repeatability of 97%. Unfortunately, the small Canadian Indigenous sample size impacted the applicability of the results and further research will be required before the technique can be used to aid in the identification of MMIWG in Canada.

This review discusses microbial forensics as an emerging science that finds application in protecting human health. It is important to distinguish naturally acquired infections from those caused by the intentional release of microorganisms to the environment. This information is crucial in formulating procedures against the spread of infectious diseases and prosecuting persons who may be involved in acts of biocrime, bioterrorism, or biowarfare. A comparison between epidemiological investigations and microbial forensic investigations is provided. In addition, a discussion on how microbial forensics strengthens health systems is included in this review. Microbial forensic investigations and epidemiologic examinations employ similar concepts and involve identifying and characterising the microbe of interest. Both fields require formulating an appropriate case definition, determining a pathogen’s mode of transmission, and identifying the source(s) of infection. However, the two subdisciplines differ in their objectives. An epidemiological investigation aims to identify the pathogen’s source to prevent the spread of the disease. Microbial forensics focuses on source-tracking to facilitate the prosecution of persons responsible for the spread of a pathogen. Both fields use molecular techniques in analysing and comparing DNA, gene products, and biomolecules to identify and characterise the microorganisms of interest. We included case studies to show methods used in microbial forensic investigations, a brief discussion of the public significance of microbial forensic systems, and a roadmap for establishing a system at a national level. This system is expected to strengthen a country’s capacity to respond to public health emergencies. Several factors must be considered in establishing national microbial forensic systems. First is the inherent ubiquity, diversity, and adaptability of microorganisms that warrants the use of robust and accurate molecular typing systems. Second, the availability of facilities and scientists who have been trained in epidemiology, molecular biology, bioinformatics, and data analytics. Human resources and infrastructure are critical requirements because formulating strategies and allocating resources in times of infectious disease outbreaks must be data-driven. Establishing and maintaining a national microbial forensic system to strengthen capacities in conducting forensic and epidemiological investigations should be prioritised by all countries, accompanied by a national policy that sets the legislative framework and provides for the system’s financial requirements. Key pointsMicrobial forensics and molecular epidemiology employ similar molecular analytical techniques in studying microbial infectious agents. Still, they differ in the rigor of standard, the requirement for a chain of custody of samples, and the strength of accredited operational procedures.National microbial forensic systems are envisioned to monitor incidents of microbial infections in a given population. Biosurveillance takes notice of emerging infectious diseases and those already controlled previously but are re-emerging in the population.National microbial forensic systems must address the challenges related to the inherent diversity of microorganisms, the requirement for robust molecular typing systems, and the availability of scientists with technical expertise in microbial forensics, molecular biology, bioinformatics, and data analytics.

The objective of this study was to compare the outcomes of nonfatal road traffic accidents by the victims’ age group and sex. We used the Portuguese medico-legal rules for personal injury assessment, in the scope of the Civil Law in that country, which includes a three-dimensional methodology. This was a retrospective study including 667 victims of road traffic accidents aged 3–94 years old. Their final medico-legal reports all used the Portuguese methodology for personal injury assessment. Outcomes were analysed by the victims’ age group (children, working-age adults, and older people) and sex. Road traffic accidents were generally serious (ISS mean 9.5), with higher severity in children and older people. The most frequent body sequelae were musculoskeletal (64.8%), which were associated with functional and situational outcomes. Temporary damage resulted in an average length of impairment of daily life of 199.6 days, 171.7 days to return to work, and an average degree of quantum doloris (non-economic damage related to physical and psychological harm) of 3.7/7. The average permanent damage was 7.3/100 points for Permanent Functional Deficit, 0.43/3 for Permanent Professional Repercussion, 2/7 for Permanent Aesthetic Damage, 3.9/7 for Permanent Repercussion on Sexual Activity and 3.2/7 for Permanent Repercussion on Sport and Leisure Activities. Overall, 19% of people became permanently dependent (10.6% needed third-party assistance). The medico-legal methodology used, considering victims’ real-life situation, allows a comprehensive assessment. There were several significant differences among the three age groups but none between sexes. These differences and the impact of the more severe cases justify further detailed medico-legal studies in these specific situations on children, older people, and severely injured victims.Key points:This was a retrospective study of accident mechanisms and injury outcomes in Portugal, and considered the outcomes in the victims’ real-life situation.Lesions from road traffic accidents were generally serious, with higher severity among children and older people.The most frequent sequels were musculoskeletal, and associated with functional and situational outcomes.Both temporary and permanent outcomes had repercussions for the victims.There were significant differences between children, working-age adults and older people, but none between sexes.

Diseases not only bring troubles to people’s body functions and mind but also influence the appearances and behaviours of human beings. Similarly, we can analyse the diseases from people’s appearances and behaviours and use the personal medical history for human identification. In this article, medical indicators presented in abnormal changes of human appearances and behaviours caused by physiological or psychological diseases were introduced, and were applied in the field of forensic identification of human images, which we called medical forensic identification of human images (mFIHI). The proposed method analysed the people’s medical signs by studying the appearance and behaviour characteristics depicted in images or videos, and made a comparative examination between the medical indicators of the questioned human images and the corresponding signs or medical history of suspects. Through a conformity and difference analysis on medical indicators and their indicated diseases, it would provide an important information for human identification from images or videos. A case study was carried out to demonstrate and verify the feasibility of the proposed method of mFIHI, and our results showed that it would be important contents and angles for forensic expert manual examination in forensic human image identification.

Accurate sex estimation is crucial to determine the identity of human skeletal remains effectively. Here, we developed convolutional neural network (CNN) models for sex estimation on virtual hemi-pelvic regions, including the ventral pubis (VP), dorsal pubis (DP), greater sciatic notch (GSN), pelvic inlet (PI), ischium, and acetabulum from the Han population and compared these models with two experienced forensic anthropologists using morphological methods. A Computed Tomography (CT) dataset of 862 individuals was divided into the subgroups of training, validation, and testing, respectively. The CT-based virtual hemi-pelvises from the training and validation groups were used to calibrate sex estimation models; and then a testing dataset was used to evaluate the performance of the trained models and two human experts on the sex estimation of specific pelvic regions in terms of overall accuracy, sensitivity, specificity, F1 score, and receiver operating characteristic (ROC) curve. Except for the ischium and acetabulum, the CNN models trained with the VP, DP, GSN, and PI images achieved excellent results with all the prediction metrics over 0.9. All accuracies were superior to those of the two forensic anthropologists in the independent testing. Notably, the heatmap results confirmed that the trained CNN models were focused on traditional sexual anatomic traits for sex classification. This study demonstrates the potential of AI techniques based on the radiological dataset in sex estimation of virtual pelvic models. The excellent sex estimation performance obtained by the CNN models indicates that this method is valuable to proceed with in prospective forensic trials.Key points Deep learning can be a promising alternative for sex estimation based on the pelvis in forensic anthropology. The deep learning convolutional neural network models outperformed two forensic anthropologists using classical morphological methods. The heatmaps indicated that the most known sex-related anatomic traits contributed to correct sex determination.

As forensic humanitarian and forensic human rights anthropology has continued to evolve, an ongoing concern in the field is meaningful engagement with survivors and the imperative to do no harm. For forensic anthropologists attempting to engage in grassroots forensic intervention, unaffiliated with an international investigation, means for effectively accessing and engaging communities has not been widely discussed. Here, forensic anthropologists draw on multiple, cross-cultural contexts to discuss methods and techniques for introducing forensic partnerships to communities. To do this, the scientist must consider their positionality as well as that of the stakeholders, develop effective local relationships, and consider a community-grounded approach. This paper argues that drawing on broader cultural anthropological training, ultimately informs one’s ability to gain entry into at-risk and vulnerable communities while minimizing harm. To illustrate this point, examples are drawn from Canada, Uganda, Cyprus, and Somaliland.

Investigating missing persons who are presumed dead in conflict contexts almost always involves witness interviews. Interviews can be critical both to locating burial sites and to positively identifying bodies that are located. Despite the importance of interviews, the authors have found that investigators in these contexts seldom have formal training on interviewing. This article highlights three principal problems that the authors have experienced relative to interviewing as part of missing persons investigations in armed conflict contexts: that interviewing is not treated as a professional activity; the tendency to overvalue technical tools in the search for burial sites; and a lack of awareness about cultural and contextual factors that impact interviews. The article concludes with five recommendations on how to improve witness interviewing.

In this research, genotyping data of 43 InDel loci in 311 Han individuals in Ankang city, Shaanxi province, China were detected using a self-developed five-dye multiplex amplification panel. The allelic frequencies and forensic parameters of all InDel loci were calculated. The combined power of discrimination and probability of exclusion values were 0.999 999 999 999 999 998 827 39 and 0.999 887 424, respectively, which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population. Moreover, genetic distances, pairwise FST values, principal component analyses, phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups. Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups.

The emergence of the Internet has transformed all areas of society. This includes the universe of scientific publications, with several publishers now exclusively focusing on the electronic format and open access model while expanding to a megajournal scope. In this context, the pandemic of predatory open access journals (POAJs) and meetings are of grave concern to the academic and research community. This new shift within academia produces a variety of new victims; namely, the authors themselves. In turn, scientific knowledge is often discredited, with the public placing less trust in science. Now more than ever, performing research with integrity and selecting a journal in which to publish requires close attention and expertise. The “predatory movement” has developed increasingly sophisticated techniques for misleading people into believing what seem to be credible professional layouts and legitimate invitations. Initiatives such as the Jeffrey Beall’s list, the Cabell’s Scholarly Analytics and Think.Check.Submit offer some guidance to uncover the “parasitic” intervention of predatory journals and meetings, but specific education in this field is sorely needed. This work aims to review the main characteristics of predatory journals and meetings and to analyze this topic in the context of forensic and legal medicine research.

A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid–liquid microextraction (DLLME) and capillary electrophoresis (CE) with photodiode array detection. The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent (chloroform) dissolved in water-miscible organic dispersive solvent (isopropyl alcohol). The organic phase, which extracted eight drugs of abuse from the prepared urine solution, was separated by centrifugation. The sedimented phase was transferred into a small volume CE auto-sampler vial with 10 µL of 1% HCl methanol solution and evaporated to dryness. The residue was reconstituted in lidocaine hydrochloride (internal standard) aqueous solution and introduced by electrokinetic injection into CE. Under the optimum conditions, acceptable linear relationship was observed in the range of 3.0–500 ng/mL with the correlation coefficient (r) of 0.9982–0.9994 for spiked urine samples. The limit of detection (LOD) (S/N = 3) was estimated to be 1.0 ng/mL. A recovery of 75.7%–90.6% was obtained for spiked samples. The mean relative error (MRE) was within ±7.0% and the relative standard deviation (RSD) was less than 6.9%. The proposed DLLME-CE procedure offers an alternative analytical approach for the sensitive detection of drugs of abuse in real urine samples. Key points: The dispersive liquid-liquid microextraction (DLLME) was involved for the determination of drugs in urine with capillary electrophoresis with photodiode array detection (CE-PDA). Good linearity, sensitivity, recovery and precision were achieved. The proposed method was eco-friendly with microliter scale solvent consumption.

Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 produced a global pandemic with significant mortality. As autopsies are not routinely performed on all decedents with SARS-CoV-2 infection, postmortem CT (PMCT) may be valuable to provide additional information on the cause of death and risk factors known to be associated with an increased mortality in COVID-19. The purpose of this manuscript is to review the PMCT findings in a series of 42 decedents with SARS-CoV-2 infection from our institution. Retrospective analysis of 42 decedents who had a positive postmortem nasopharyngeal swab for SARS-CoV-2 and had a PMCT were included in this study. Images were reviewed for pulmonary findings seen in COVID-19 and other organ involvement. Of the 42 decedents, although the majority had imaging findings in the lungs that would be consistent with COVID-19 and acute respiratory distress syndrome, in 14% of the decedents the SARS-CoV-2 infection was likely coincidental and the PMCT findings suggested that they died from other pathology. Over half of the decedents that died from COVID-19 had PMCT findings of vascular disease. PMCT is useful to identify pulmonary and extra pulmonary findings in decedents with SARS-CoV-2 infection that can provide additional information, which may be useful for the forensic pathologist to help determine the underlying cause of death. Supplemental data for this article are available online at

With the development and maturation of massively parallel sequencing (MPS) technology, the mitochondrial genome (mitogenome) sequencing is increasingly applied in the forensic field. In this study, we employed the strategy of short overlapping amplicons for the whole mitogenome, library preparation with tagmentation using the Nextera® XT DNA Library Preparation Kit, sequencing on the MiSeq FGxTM Forensic Genomics System and analyzing data using the mitochondrial(mtDNA) MSR Plug-in and the mtDNA Variant Analyzer. A total of 27 libraries and 56 libraries were sequenced in a run using MiSeq Reagent Kit v2 and v3, respectively. Results showed more than 1800 × of averaged depth of coverage (DoC) at each position. Concordant haplotypes of 9947 A and 2800 M were obtained at 32 variants. Cross-reactivity was observed with 1 ng primate DNA and 10 ng non-primate DNA but could be easily distinguished. Full and accurate variants were obtained from at least 50 pg input DNA and from minor contributors between 19:1 and 1:19 mixed ratios with known reference profiles. More than 86% variants were detected from ≥200-bp degraded samples but its haplotype was assigned to more ancestral haplogroup. Further, a total of 3 962 variants were observed at 613 nucleotide positions from 103 Xibe mitogenomes with 25:1 ratio of transitions to transversions. Two new transversions (C13735A and A14755C) and two tri-alleles at nps 9824 and 16092 were identified. There were 103 unique mitogenome haplotypes from 103 Chinese Xibe that were assigned to 79 haplogroups. Haplogroup D was the preponderant top-level haplogroup in Xibe followed by F, B, M, A, N, G, C, Z, Y, HV and J. Random match probability (RMP) and haplotype diversity (HD) of the whole mitogenome was calculated as 0.0097 and 1.0000, respectively. Compared with HVS-I only, RMP decreased 33.56%, while the number of haplotypes and HD increased 15.73% and 0.49%, respectively. Principal component analysis (PCA) showed that Xibe was clustered to East and Southeast Asian. As a whole, this MPS strategy is suitable for the whole mitogenome sequencing especially for degraded samples and can facilitate generating mitogenome data to support the routine application in forensic sciences. EMP00726 is the first whole mitogenome dataset from Xibe contributed to the EMPOP.

Identification of unknown remains recovered from marine and terrestrial locations is a significant humanitarian problem. This investigation proposes a simple method applicable to fragmentary femora for a more refined level of ancestry and/or sex estimation. To that end, we re-examined Purkait’s triangle which involves three inter-landmark distances between the traction epiphyses and the articular rim of femoral head. A large sample (n = 584) from geographically diverse (Egyptian, Indian and Greek) populations was compiled. Additionally, shape (n = 3) and trigonometrically derived variables and ratios (n = 9 variables) were employed to detect any geographically-clustered morphological differences between these populations. Random forest modelling (RFM) and linear discriminant function analysis (LDA) were employed to create classification models in instances where sex was known or unknown. The sample was apportioned into training and test sets with a ratio 70/30. The classification accuracies were evaluated by means of k fold cross-validation procedure. In sex estimation, RFM showed similar performance to LDA. However, RFM outperformed LDA in ancestry estimation. Ancestry estimation was satisfactory in the Indian and Egyptian samples albeit the Greek sample was problematic. The Greek samples presented greater morphological overlap with the Indian sample due to high within-group variation. Test samples were accurately assigned to their ancestral category when sex was known. Generally, higher classification accuracies in the validation sample were obtained in the sex-specific model of females than in males. Using RFM and the linear variables, the overall accuracy reached 83% which is distributed as 95%, 71% and 86% for the Egyptian, Indian and Greek females, respectively; whereas in males, the overall accuracy is 72% and is distributed as 58%, 87% and 50% for the Egyptian, Indian and Greek males, respectively. Classification accuracies were also calculated per group in the test data using the 12 derived variables. For the females, the accuracies using the medians model was comparable to the linear model whereas in males the angles model outperformed the linear model for each group but with similar overall accuracy. The classification rates of male specific ancestry were 82%, 78% and 56% for the Egyptian, Indian and Greek males, respectively. In conclusion, Purkait’s triangle has potential utility in ancestry and sex estimation albeit it is not possible to separate all groups successfully with the same efficiency. Intrapopulation variation may impact the accuracy of assigned group membership in forensic contexts. Key points Purkait’s method is a possible ancestry group indicator applicable to fragmentary femora. Random forest model surpassed linear discriminant function analysis in multi-group ancestry classification. Ancestry is more accurately assessed in females than males. The intertrochanteric distance is the most important feature in discrimination of sex whereas in ancestry it was the head to lesser trochanter distance. Sex differences override ancestry due to the tendency of misclassification into same sex but different group rather than the opposite sex of the same ancestry.

Intimate partner violence (IPV) is simultaneously assumed as a serious crime and a major public health issue, having recurrences as one of its main characteristics and, consequently, re-entries of some alleged offenders in the criminal justice system (CJS). The main goal of this study is to assess if in cases of female victims of IPV, violence decreases after the first entry of the alleged offender in the CJS. A retrospective study was performed based on the analysis of police reports of alleged cases of IPV during a 4-year period. The final sample (n = 1 488) was divided into two groups according to the number of entries in the CJS (single or multiple) followed by a comparative approach. Results suggest that violence decreases after the first entry of alleged offenders in the CJS. Re-entries were found in only 15.5% of the cases but they were accountable for 3.3 times more crimes on average. Besides, victims of recidivism presented more injuries and required more medical care. Thus, a small group of alleged offenders seems to be more violent and accountable for most of the IPV crimes registered in the CJS suggesting that regardless of legal sanctions aiming to deter violence, these measures may not be enough for a certain group of offenders. This study sustains the need for a predictive model to quantify the risk of repeated IPV cases within the Portuguese population.

The National Research Council recommends that genetic differentiation among subgroups of ethnic samples be lower than 3% of the total genetic differentiation within the ethnic sample to be used for estimating reliable random match probabilities for forensic use. Native American samples in the United States’ Combined DNA Index System (CODIS) database represent four language families: Algonquian, Na-Dene, Eskimo-Aleut, and Salishan. However, a minimum of 27 Native American language families exists in the US, not including language isolates. Our goal was to ascertain whether genetic differences are correlated with language groupings and, if so, whether additional language families would provide a more accurate representation of current genetic diversity among tribal populations. The 21 short tandem repeat (STR) loci included in the Globalfiler® PCR Amplification Kit were used to characterize six indigenous language families, including three of the four represented in the CODIS database (i.e. Algonquian, Na-Dene, and Eskimo-Aleut), and two language isolates (Miwok and Seri) using major population genetic diversity metrics such as F statistics and Bayesian clustering analysis of genotype frequencies. Most of the genetic variation (97%) was found to be within language families instead of among them (3%). In contrast, when only the three of the four language families represented in both the CODIS database and the present study were considered, 4% of the genetic variation occurred among the language groups. Bayesian clustering resulted in a maximum posterior probability indicating three genetically distinct groups among the eight language families and isolates: (1) Eskimo, (2) Seri, and (3) all other language groups and isolates, thus confirming genetic subdivision among subgroups of the CODIS Native American database. This genetic structure indicates the need for an increased number of Native American populations based on language affiliation in the CODIS database as well as more robust sample sets for those language families. Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1963088 .

In December 2017, a decomposed unidentified body was found near the river Tronto in Teramo, Italy. The corpse was found without any identifying documents or specific personal belongings, except for a packet of cigarettes. The medical examiner determined a gastric perforation from the intake of hydrochloric acid to be the cause of death. A jar of muriatic acid found near the body led to suicide being considered the manner of death. The Penal Court in Teramo appointed two forensic odontologists to complete the postmortem assessment and collect dental data for personal identification. The corpse was found wearing a complete set of upper and lower dentures. The dental autopsy and 42 periapical X-ray images helped generate a biological profile of a man totally edentulous with upper and lower dentures, as well as an osteosynthesis with two plates and screws in the left ascending ramus of the mandible. In March 2018, the sister of a missing person reported the disappearance of her brother, and a presumptive identification was performed through visual recognition of the decomposed body. The sister confirmed the presence of two dentures and the location of the maxillo-facial surgery for the treatment of the fractured mandible. A complete dental autopsy was able to establish his identity without any DNA comparison needed. This case highlights the importance of performing a complete dental autopsy inclusive of dental radiographs, and its value in the identification of all unknown human remains even when totally edentulous. A complete dental autopsy should be performed in all cases of human identification.Keypoints Despite a corpse being edentulous, a complete dental autopsy can still be useful. Dental radiographs, such as bitewings, periapical images, panoramic radiographs, and CT scans, are recommended in all identification autopsies.

Deaths caused by barbiturate overdoses have increased in the past decade, especially as a result of suicide attempts. Pentobarbitone is a central nervous system depressant used for sedation and euthanasia in veterinary medicine. However, pentobarbitone analysis is not commonly available in the hospital setting; hence, its occurrence in overdoses is under-reported. Herein we describe a patient who ingested pentobarbitone obtained from the Internet with the purpose of ending his life. He became comatose and required ventilation for 6 days. While critically ill, the drug and a barbiturate test kit were found in his room at his residence. Toxicological analysis of the patient’s blood determined the presence of pentobarbitone at levels of 91, 56, and 19 mg/L at 11, 59, and 107 h after ingestion, respectively. With supportive care, the patient made a full recovery. He stated that he believed the liquid was to be pentobarbitone, and that he had received advice on its use from an online forum that he had found on a dark web marketplace. In this report, we highlight the process by which we facilitated pentobarbitone analysis with a rapid turnaround time, which helped to inform clinical management and raise awareness among clinicians. The access was made through the Prescription, Recreational and Illicit Substance Evaluation (PRISE) programme, which is a collaborative network among the New South Wales (NSW) Ministry of Health, NSW Poisons Information Centre (PIC), and NSW Health Pathology Forensic & Analytical Science Service (FASS).Key points • We report a patient with confirmed severe pentobarbitone toxicity who developed coma, respiratory failure, barbiturate related skin and vascular manifestations who required intensive care for 6 days.• The diagnosis of pentobarbitone poisoning can be missed as it is not routinely included in Australian standard hospital urine drug screens, and it may not cross-react with phenobarbitone testing which may be more readily available. • Timely access to comprehensive toxicology testing with rapid turnaround time assists diagnosis for unknown toxicity, and enhances case management and public health interventions. • The PRISE programme in Australia is a collaboration between multiple health functional units in NSW, Australia that provides timely access to extensive toxicology testing for severe and unusual toxicity from drugs or substance-related toxicities.

Hair analysis has been mainly used to document drug use history in abusers, drug-facilitated crime cases, doping control analysis and postmortem toxicology in the fields of forensic toxicology, clinical toxicology, and doping control. Hair analysis has also gained more attention in the last 30 years in China. Relevant technology has been promoted as more research has appeared concerning hair analysis, and consensus has been sought among forensic toxicologists regarding aspects such as hair decontamination treatment, detection of abused substances in hair, segmental hair analysis and interpretation of analytical results. However, there are still some limitations in the estimation of drug intake time and frequency by segmental hair analysis due to the different growth cycles evident within a bundle of hairs, the drug incorporation mechanism and sampling errors. Microsampling and imaging mass spectrometry (IMS) technology based on a single hair may be a good choice to estimate drug intake time more accurately. Analysis of hair root samples may also be used to document acute poisoning in postmortem toxicology, and the analysis of the hair shaft can document long-term use of drugs depending on the length of the hair being evaluated.

To investigate effects of smoking cannabidiol (CBD)-rich marijuana on driving ability and determine free CBD and Δ9-tetrahydrocannabinol (THC) concentrations in capillary blood samples, a randomised, double-blind, placebo-controlled, two-way crossover pilot study was conducted with 33 participants. Participants smoked a joint containing 500 mg of tobacco and either 500 mg of CBD-rich marijuana (16.6% total CBD; 0.9% total THC) or 500 mg of a placebo substance, then performed three different dimensions of the Vienna Test System TRAFFIC examining reaction time, behaviour under stress, and concentration performance. For further assessment of participants’ fitness to drive, three tests of balance and coordination were evaluated and vital signs (blood pressure and pulse) were measured. Dried blood spot samples of capillary blood were taken after smoking and after completion of the tests to determine the cannabinoid concentrations (CBD, THC and THC-metabolites). The results revealed no significant differences between the effects of smoking CBD-rich marijuana and placebo on reaction time, motor time, behaviour under stress, or concentration performance. Maximum free CBD and THC concentrations in capillary blood were detected shortly after smoking, ranging between 2.6–440.0 ng/mL and 6.7–102.0 ng/mL, respectively. After 45 min, capillary blood concentrations had already declined and were in the range of 1.9–135.0 ng/mL (free CBD) and 0.9–38.0 ng/mL (free THC). Although the observed levels of free THC concentrations have been reported to cause symptoms of impairment in previous studies in which THC-rich marijuana was smoked, no signs of impairment were found in the current study. This finding suggests that higher CBD concentrations cause a negative allosteric effect in the endocannabinoid system, preventing the formation of such symptoms. Nevertheless, it is recommended that consumers refrain from driving for several hours after smoking CBD-rich marijuana, as legal THC concentration limits may be exceeded. Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1946924 .

This study presents a detailed model and its subsequent assessment for the development and optimized delivery of continuing education for the forensic science community. Online continuing education is on the rise as this modality of content dissemination allows for accessibility, convenience, and affordability. The model, which includes an educational needs assessment, gap analysis, online content delivery and archival, was evaluated through the launch of a global training event/symposium series. The four-year study of over 6 000 participants representing >75 countries provides empirical support for the model based upon registration/attendance data and participant self-assessment surveys. Best practices are identified relative to content and format, operational excellence, technology, and financing of the event. Future directions along with opportunities to further enhance the model are also discussed.Key Points A model is presented for the development, online delivery, and archival of compelling, cost effective continuing education content for the forensic science community. The model is evaluated based upon registration/attendance data and participant surveys. Best practices relative to content and format, operational issues, selection of technology and financing of the event are shared.

3-Methoxyphencyclidine (3-MeO-PCP) is a new psychoactive substance that belongs to the phencyclidines family, first identified in Europe in 2012. This drug presents a stronger binding to N-methyl-D-aspartate (NMDA) receptors when compared to phencyclidine, which results in more potent effects, even at low concentrations. Very few articles have been published regarding 3-MeO-PCP in forensic toxicology. In this paper, the authors present a fatal 3-MeO-PCP intoxication case. In addition to the detection of the parent drug, metabolites were investigated in urine and, for the first time in the scientific literature, in blood. 3-MeO-PCP and its metabolites were quantitated by liquid chromatography-tandem mass spectrometry system (LC-MS/MS). Identification was confirmed by liquid chromatography-high resolution mass spectrometry (LC-HRMS). 3-MeO-PCP tested positive in femoral blood (3 525 ng/mL) and urine (7 384 ng/mL). The femoral blood concentration was higher than the fatal concentrations range already reported in the literature (from 50 to 3 200 ng/mL). 3-MeO-PCP metabolites, including O-demethyl-3-MeO-PCP, piperidine-OH-3-MeO-PCP, O-demethyl-piperidine-di-OH-3-MeO-PCP and piperidine-di-OH-3-MeO-PCP, were detected in blood. In addition, two new metabolites, O-demethyl-piperidine-OH-3-MeO-PCP and O-demethyl-cyclohexyl-OH, were identified in both blood and urine. Unfortunately, due to the lack of reference material on the market, it was not possible to measure the concentration of these metabolites. However, the ratios between the metabolites and the parent drug were useful to estimate their analytical response and prevalence. At this time, considering the low ratios (

In recent years, a significant number of investigations have discovered up to 200 000 unsubmitted sexual assault kits (SAKs) in the US. While the public outcry was largely directed towards DNA analysis, the SAKs also contained biological specimens specifically designated for toxicological analysis. Due to the sensitivity of analytes in potential drug facilitated sexual assaults, the preservation and maintenance of the specimens is crucial in providing accurate toxicological measurements. The investigations into the unsubmitted SAKs have identified subjective law enforcement officer (LEO) rationale for the unsubmitted kits, however the impact on toxicological specimens has not been examined. This brief review of policies and guidelines with respect to forensic specimens has identified potential sources of evidentiary degradation, despite the use of chemical preservatives. With respect to temperature-controlled environments, the variation in SAK submission policies established throughout the US are potentially detrimental to the preservation of toxicological evidence. Degradation as a result of time-delayed collection and poorly maintained storage temperatures plays a crucial role for/in the interpretation of qualitative and quantitative toxicological results. This review finds these delays can be addressed through modernisation of facilities; electronic tracking of unsubmitted SAKs; mandated transfer of biological evidence within 72 h; and documentation of temperature within the chain of custody or other records. Without identifying the range of temperatures in which the evidence was exposed, forensic toxicologists may unintentionally provide erroneous interpretations of toxicological analyses – potentially casting doubt on the survivor’s recall of events and negatively impacting future sexual assault investigations.

This work presents an odd historical record obtained through more than 14 years of research regarding one of the first major European forensic cases. The presumed homicide of Mário Guilherme Augusto de Sampaio in 1890 was allegedly perpetrated by his uncle, the prestigious doctor Vicente Urbino de Freitas. This famous poisoning had international repercussions for decades, with the participation of several forensic experts that made the history of forensic sciences, namely forensic toxicology and pathology. This third work aims to collect, restore, and analyse all the forensic evidence, particularly from the autopsy, toxicological, and psychiatric forensic reports. Facts regarding the life of Vicente Urbino de Freitas during his exile in Brazil were also recovered, along with a vast and outstanding assortment of forensic medicine photographs from the 19th century.

A novel hair sample pre-treatment method based on high-speed grinding and solid-phase microextraction (SPME) had been applied for the determination of amphetamines, ketamine and their metabolites in hair samples by liquid chromatography-mass spectrometry (LC-MS). A 20 mg sample of hair was ground with 2 mL of saturated sodium carbonate solution using a high-efficiency hair grinder with 70 Hz oscillation for 2 min at 4 °C. After centrifuging, 1.5 mL of the supernatant was transferred and treated with SPME by direct immersion (DI-SPME). The target analytes extracted by fibre were desorbed and analysed using LC-MS. Under the optimum conditions, a recovery of 90.2%–95.8% was obtained for all analytes. The analytical method was linear for all analytes in the range from 0.2 to 10 ng/mg with the correlation coefficient ranging from 0.9985 to 0.9993. The detection limits for all analytes were estimated to be 0.067 ng/mg. The accuracy (mean relative error) was within ±6.9% and the precision (relative standard error) was less than 6.8%. The combination of high-speed grinding of hair and SPME had the advantages of being easy to perform, environment-friendly and high in detection sensitivity. The proposed method offered an alternative analytical approach for the sensitive detection of drugs in hair samples for forensic purposes.Key Points The SPME was involved for the determination of drugs in hair with LC-MS. The hair high-speed grinding combined with SPME was firstly developed. Good linearity, sensitivity, recovery and precision were achieved.

Insertion/deletion polymorphisms (InDels) have been treated as a prospective and helpful genetic marker in the fields of forensic human identification, anthropology and population genetics for the past few years. In this study, we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application. The contained InDels were specifically selected for Chinese population with the MAF ≥ 0.25 in East Asia, which do not overlap with the markers of Investigator® DIPplex kit. The typing system was named as GoldeneyeTM DNA ID System 35InDel Kit, and a series of developmental validation studies including repeatability/reproducibility, concordance, accuracy, sensitivity, stability, species specificity and population genetics were conducted on this kit. We confirmed that the 35InDel kit is precise, sensitive, species specific and robust for forensic practice. Moreover, the 35InDel kit is capable of typing DNA extracted from forensic routine case-type samples as well as degraded samples and mixture samples. All markers are proved to be highly polymorphic with an average observed heterozygosity (He) of 0.4582. The combined power of discrimination (CPD) is 0.999 999 999 999 978 and the combined power of exclusion in duos (CPED) and trios (CPET) are 0.978 837 and 0.999573, respectively, which are higher than those of the Investigator® DIPplex kit. Thus, the GoldeneyeTM DNA ID System 35InDel kit is suitable for forensic human identification and could serve as a supplementary typing system for paternity testing. Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1945723 .

In 1993, the skeletal remains of a female corpse were found in Burgenland, Austria. Initial identification of the approximately 25–35-year-old female appeared impossible, but the case was reopened 23 years later. By applying biogeochemical isotope methods to her body tissues, the geographical origin of the unknown corpse could be predicted. The results of the C, N, S, H, Sr, and Pb isotope analyses suggested that the female did not originate from Europe and most likely spent her youth in the northern Caribbean. Using these findings, the police were able to identify the woman within 2 weeks. The female came from the Dominican Republic and resided in Austria for only a short period before she was murdered. This case shows that isotope biogeochemistry investigations can provide the police with crucial information that enables unknown persons to be identified. Keypoints C-N-S-H and Sr-Pb isotope analyses were applied to human remains associated with a cold case. It was possible to determine the region of origin of the unknown deceased individual as the northern Caribbean. After 23 years, the murder victim was successfully identified.

We investigated the forensic efficacy of the 30 insertion/deletion (Indel) markers included in the Qiagen Investigator® DIPplex kit in 529 Pakistani individuals from five major subpopulations in Pakistan (Punjabi, Pashtun, Sindhi, Saraiki, and Baloch). In the Sindhi population, the distribution of HLD81 and HLD97 alleles deviated from Hardy-Weinberg equilibrium after Bonferroni correction. The combined match probability ranged from 2.0E-12 (Pashtun and Baloch) to 1.0E-12 (Sindhi), and the mean paternity exclusion power varied from 0.995 (Punjabi, Sindhi, and Saraiki) to 0.996 (Pashtun and Baloch). The high combined power of discrimination (0.999 999 999 999 97) and low combined match probability (1.7E-12) for all subpopulations studied support the utility of the 30 Indel markers for forensic identification in the studied subpopulations. The allele frequencies of the Indel markers in the Pakistani subpopulations were compared with those from 18 other populations. The results show that the populations clustered according to geography. The subpopulations investigated in this work showed a close genetic relationship with others from Pakistan, as well as with South Central Asian and Middle Eastern populations. The results suggest that the Investigator® DIPplex kit can be useful as a supplementary tool for human identification in the five Pakistani subpopulations investigated in this study. Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.1933366 .

The use of electronic signatures as a form of identification is increasingly common, yet they have been shown to lack the dynamic features found in online signatures. In this study, handwritten signatures were scanned to produce electronically scanned signatures (ESS) which were then digitally altered to produce digitally constructed signatures (DCS). The ESS and DCS were presented back to participants to identify which were genuine. Only 1% of participants correctly identified all signatures, with a mean score of 57.6% identifications. The lack of self-recognition of ESS raises questions on their reliability and usefulness as means of personal identification.

Forensic science is crucial for the administration of justice and case investigation. In China, political-legal organizations, including the courts, public security, procuratorate, and judicial administration, developed their own forensic practices before 2004. As a result, the frequent and repeated appraisals undermined judicial authority and credibility. Thus, a law was published in 2005 to improve the uniform forensic management system by the Standing Committee of the National People’s Congress, leading to the establishment of the Forensic Administration of the Ministry of Justice in 2006. During this process, the increased accreditation and interflow highlighted the role of consensus in forensic standards for forensic service providers to avoid uncertainty regarding the methods used and interpretation of results. In 2017, a policy document was promulgated again to strengthen the importance of the uniform standards, which also proposed to establish a new national technical committee for the standardization of forensic science by the General Office of the State Council. In 2018, despite the continuing problems concerning uniformity, the Forensic Administration of the Ministry of Justice was merged into the Public Legal Services Administration. Yet, there is still a long way to go for the national technical committee for the standardization of forensic science. This paper analyses the evolution of forensic standards internationally and nationally, discusses the existing problems, and proposes relative solutions. Moreover, it discusses the future of standards development with the deepening of the reformation of both the national standardization and judicial system.

Functional near-infrared spectroscopy (fNIRS) is a technique of detecting cerebral cortical function by using near-infrared light, which is a multifunctional neuroimaging technique and provides a convenient and efficient detection method in neuroscience. In consideration of acceptability, safety, high spatial and temporal resolutions compared with electroencephalogram (EEG) and functional magnetic resonance imaging (fMRI), fNIRS is widely used to study different psychiatric disorders, most prominently affective disorders, schizophrenic illnesses, brain organic mental disorders and neurodevelopmental disorders, etc. The article focuses on the latest research progress and practical application of fNIRS in psychiatric disorders, especially traumatic brain, including studies on the characterization of phenomenology, treatment effects and descriptions of neuroimaging data.

Eye tracking is used in sexology to identify attractiveness and sexual desire indirectly. This systematic review summarizes results of works that have used eye tracking to analyse paedophilic interest in order to investigate its potential as a useful forensic tool. Six studies met the inclusion criteria. Five of them concerned a large study project and used approximatively the same sample of paedophiles (inpatients), forensic patients (without a sexual interest in children) and healthy controls to make comparisons between the three groups. One study added 11 self-declared paedophiles (outpatients) for a comparison between inpatient paedophiles, outpatient paedophiles and controls (healthy and forensic inpatients). One study compared a group of child sexual offenders with non-offenders. All studies used static pictures of male and female subjects at different pubertal stages. Some studies divided every picture into a different area of interest. Dependent variables used are fixation latency (early attention) and relative fixation time, (later attention). Each study identified significant differences between the paedophile group versus other groups: shorter fixation latency on child pictures, longer fixation time on child pictures and number of fixation most important on child pictures. Two scores (age preference index and attentional control index) showed hight and/or moderate sensitivity and specificity. Although the results suggest the eye tracking can discriminate between paedophile interest and non-paedophile interest, there are too few studies on this specific topic and further research is needed with larger and different sample, carried out by different research teams. If these findings were confirmed, it remains unclear as to their impact in a forensic context when presented openly in Court.

It may be difficult to distinguish the cause of death in drowning cases without specific findings. The aim of this study was to explore the forensic value of thoracic postmortem computed tomography (PMCT) using routine images and three-dimensional (3D) image reconstructions. The imaging data of PMCT examinations of six drowning cadavers, aged 21–54 years, were analyzed. Twelve victims of sudden death from coronary artery disease (CAD) were chosen as a control group. After 3D bilateral lung images were reconstructed using image processing software, an interactive medical image control system was used to measure and analyze parameters including lung volume, lung volume ratio, mean CT value of the whole lung, and lung CT value distribution curves. Lung volume and lung volume ratio were used to assess the shape changes of the lung. Lung CT value distribution curves showed the corresponding number of pixels of the different CT values in the lung image. Lung volume was not significantly larger in drowning cases (mean 2 958 cm3) than in controls (mean 2 342 cm3). Lung volume ratio values in the drowning group (mean 0.3156) were greater than those in the control group (mean 0.2763); (P = 0.02). There was no significant difference between the drowning and control group in the mean CT value of the whole lung. There were differences between lung CT value distribution curves in drowning victims and controls, with drowning victims showing a single peak and CAD cases showing a bimodal distribution. Thoracic PMCT is helpful for the forensic medical diagnosis of drowning. Lung volume ratio and lung CT value distribution are potential indicators to distinguish between drowning and CAD.

Epigenetic mechanisms are potential mediators of the physiological response to abuse by altering the genetic predisposition of the cellular response to the environment, leading to changes in the regulation of multiple organ systems. This study was established to review the epigenetic mechanisms associated with childhood abuse as well as the long-term determinants that these epigenetic changes may have on future illness. We retrospectively analysed the effect of exposure to adverse childhood experiences (ACEs, specifically those relating to childhood maltreatment) between the ages of 0 and 16 years on the human epigenome, as well as possible clinical associations. After meeting inclusion and exclusion criteria, 36 articles were included in this systematic review. Eight of these studies did not find a relationship between childhood maltreatment and DNA methylation. Of the remaining 28 studies, nine were genome-wide association studies, whereas the rest were candidate gene studies, mainly studying effects on neuroendocrine, serotoninergic and immunoregulatory systems. Meta-analysis of correlation coefficients from candidate gene studies estimated an association of childhood adversity and DNA methylation variation at r = 0.291 (P