1. Occurrence of Trypanosoma caninum in areas overlapping with leishmaniasis in Brazil: what is the real impact of canine leishmaniasis control?
- Author
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Barros JH, Almeida AB, Figueiredo FB, Sousa VR, Fagundes A, Pinto AG, Baptista C, and Madeira MF
- Subjects
- Animals, Brazil epidemiology, Chagas Disease epidemiology, Chagas Disease prevention & control, Communicable Disease Control, Dog Diseases prevention & control, Dogs, Endemic Diseases veterinary, Euthanasia, Animal, Humans, Leishmania infantum genetics, Leishmaniasis epidemiology, Leishmaniasis prevention & control, Polymerase Chain Reaction methods, Sequence Analysis, DNA, Trypanosoma genetics, Chagas Disease veterinary, Dog Diseases epidemiology, Leishmania infantum isolation & purification, Leishmaniasis veterinary, RNA, Ribosomal, 18S isolation & purification, Trypanosoma isolation & purification
- Abstract
Trypanosoma caninum is a parasite of the Trypanosoma genus recently described in the natural infection of dogs in the municipality of Rio de Janeiro, Brazil. Suspecting the existence of a natural cycle and the circulation of this new species, the objective of this study was the taxonomic identification of samples of Trypanosoma spp. isolated from dogs in different Brazilian regions. Parasites were solely obtained from skin fragments culture and characterized by nested-PCR targeting the partial sequence of 18S rRNA gene and PCR products were sequenced. Thirty-three samples, obtained in São Paulo, Minas Gerais, Goiás, Mato Grosso and Rio de Janeiro states were analyzed. PCR and sequencing showed that the isolates were genetically identical or closely similar and confirmed T. caninum identity. This report broadens the geographical distribution of T. caninum in Brazil and discusses the impact of the presence of this parasite in areas of canine leishmaniasis occurrence., (Copyright © 2012 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2012
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