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Indigestible fiber–protein–phytate complexes reduce the feeding value of soy products. We investigated the effects of multienzyme supplement (MES, Victus) on standardized ileal digestibility (SID) of amino acids (AA) and apparent total tract digestibility (ATTD) of energy and minerals in roasted full-fat soybean (FFSB) seeds and expelled-extruded soybean meal (SBM) fed to growing pigs. The crude protein (CP) was 33.4% and 42.8% dry matter (DM) in FFSB seeds and SBM, respectively and corresponding values for crude fat were 17.4% and 11.8% DM. Semi-purified diets with 50% of either FFSB seeds or SBM as the sole source of AA were prepared without or with MES supplying phytase, protease, xylanase, and β-glucanase at 2,200, 8,300, 400, and 100 U/kg of feed, respectively. Diets had TiO2 as an indigestible marker and the ratio of cornstarch to sucrose and corn oil was identical to calculate DE by the difference method. Eight ileal-cannulated barrows (22.1 ± 0.61 kg) were fed diets in a replicated 4 × 4 Latin square design to give eight replicates per diet. The period lasted for 9 d: 5 d for acclimation, 2 d for fecal, and 2 d for ileal digesta samples. There was no (P > 0.05) interaction between soy type and MES or MES effect on SID of AA; SBM had higher (P < 0.05) SID of CP, His, Leu, and Lys. There was no (P > 0.05) interaction between soy type and MES on energy digestibility. The FFSB seeds had higher ATTD of gross energy (GE, 80.2% vs. 76.6%; P < 0.01) than SBM. Pigs fed MES had higher (P < 0.05) ATTD of DM (91.3% vs. 87.7 %), GE (87.5% vs. 82.4%), CP (86.4% vs. 82.9%), crude fat (70.6% vs. 54.9%), Ca (63.2% vs. 60.2%), and P (67.5% vs. 63.2%). In conclusions, differences on AA and energy digestibility in soy products could be linked to processing and compositional differences. Although MES had no effect on SID of AA, the effects on the utilization of minerals and energy demonstrated the value of fiber-degrading enzymes, protease, and phytase in improving the nutritive value of soy products independent of processing.

A combination of in vivo and in vitro fermentation methodologies was used to determine the interactive effects of dietary fiber (DF) and lipid types on volatile fatty acids (VFA) production and absorption, and organic matter (OM) fermentability in the cecum and colorectal tract of pigs. Eight ileal- and caecal-cannulated Yorkshire barrows were fed either pectin- or cellulose-containing diets that were supplemented with either corn oil or beef tallow in two independent Youden squares with a 2×2 factorial arrangement of treatments (n = 6). Ileal and caecal digesta were collected, freeze-dried and fermented using inoculum from fresh caecal digesta and feces, respectively, to determine VFA production and absorption, and fermentability of OM. There were interactions (P < 0.001) between DF and lipid types observed in which the addition of corn oil increased the quantity of caecal and colorectal acetic acid production and caecal acetic absorption, caecal butyric production, predicted caecal OM fermentability, and the predicted colorectal propionic acid in pectin diets but did not have effects in cellulose diets. The addition of beef tallow increased (P < 0.001) the production of caecal butyric and propionic acids during in vitro fermentation in cellulose diets and fermentability of OM in pectin diets. The interactions between DF and lipids on gastrointestinal fermentation largely depends on the degree of saturation of fatty acids in dietary lipids. The addition of beef tallow selectively decreased the production and absorption of individual SCFA in pectin and cellulose diets but increased caecal butyric and propionic acid production in cellulose diets and the fermentability of OM in pectin diets.

Four commercial feed additives (CFA) were evaluated for efficacy in detoxifying deoxynivalenol (DON) in diets fed to 144 nursery pigs (BW 9.80+/- 0.5kg; 2 gilts, and 2 barrows/pen). Six test corn-soybean-meal based diets were: 1) positive control (PC), formulated with clean corn (NC), 2) formulated with contaminated corn (5.5 ppm DON), 3) NC with enzyme+binder1 (NCB1), 4) NC with clay (NCC), 5) NC with enzyme+binder2 (NCB2) and 6) NC with sodium metabisulfite (NCP). Diets were allocated to pens (n = 6) based on BW and fed ad-libitum for 4-wk; BW and feed intake were monitored weekly. At the end of wk-1, one pig/pen was euthanized for tissue collection. Assayed DON concentration for PC, NC, NCB1, NCC, NCB2 and NCP were 0.38, 2.3, 2.3, 2.3, 2.4, and 1.9 ppm, respectively. Diet effects were only observed for ADG and G:F in wk-1; pigs fed NCP had higher (P = 0.01) ADG compared to pigs fed NC and NCC, whilst pigs fed other diets had intermediate and similar ADG (P > 0.05) to pigs fed NC or NCC. Pigs fed NCP showed higher (P = 0.02) G:F relative to pigs fed other diets. The final BW was 31.3, 30.8, 30.4, 30.8, 31.1 and 32.2kg for PC, NC, NCB1, NCC, NCB2 and NCP-fed pigs, respectively. There were no (P > 0.05) diet effects on ADFI throughout the study. There was no (P > 0.05) diet effects on liver and spleen weight, jejunum morphology and concentration of plasma creatinine and urea. Ceca digesta concentration of citric acid was higher (P = 0.03) in pigs fed NCP than pigs fed NCB2. In conclusion, the lack of differences between PC and NC suggested pigs were tolerant to higher DON levels than the regulated dietary maximum of 1 ppm. Improved ADG and G:F due to NCP vs. NC was transient and was not linked to physiological responses.

Among effective nutritional factors on bone dynamics in reproducing fowl, Ca and vitamin D are very important. We investigated the effect of different levels of Ca and 25-hydroxy vitamin D3 (25OHD3) on tibia characteristics in Lohmann LSL-lite layers from 74 to 81 wk of age. Four levels of Ca (3.0, 3.5, 4.0 and 4.5% of diet) and three levels of 25OHD3 (0, 69 and 138 µg/kg of diet) were tested with a basal level of 330 KIU of vitamin D3/kg. Eighty-four individually housed hens had free access to feed and water and were sacrificed at the end of wk 81 for tibia samples. Tibia ends (spongy bone epiphysis and metaphysis) were separated from diaphysis. The ends and diaphysis were longitudinally cut and medullary bone separated by scrapping. The ends, cortical and medullary sections were dried at 105°C for measuring percentage of dried weight (PDW) of each parts to the whole tibia. Dried sub-part samples ashed at 600°C for measuring total ash content (AC) and ash concentration (ACO) of each part of tibia. The inclusion of Ca had no effect on the PDW of sub-parts of tibia (P>0.05). Conversely, 25OHD3 linearly (P=0.014) increased PDW of medullary and decreased PDW of cortical (P=0.001). There was no (P>0.05) interaction effect between Ca and 25OHD3 or Ca levels on PDW, AC and ACO in medullary and cortical part. The main effect of 25OHD3 was such that medullary AC and ACO increased (P

The use of corn distillers dried grains with solubles (DDGS) in pig diets is limited due to high fiber concentration. Steeping with exogenous fiber-degrading enzymes (FDE) may improve their feeding value. We evaluated apparent ileal digestibility (AID), standardized ileal digestibility (SID), and apparent total tract digestibility (ATTD) of components and DE content in DDGS steeped without or with two commercial FDE (A and B). Mixture of 350 g of DDGS, FDE (none for control), and 1.5 liters of water was incubated at 40 °C for 24 h with 15 min agitation every 40 min. FDE-A (pure combination) supplied 5,500 U of xylanase and 1,050 U of β-glucanase while FDE-B (multienzyme complex) supplied 1,200 U of xylanase, 150 U of β-glucanase, 500 U of cellulase, and 5,000 U of protease per kg of DDGS plus side activities. Samples were taken at time 0, 4, 8, and 24 h for organic acids and pH measurements. Three semi-purified corn starch–based diets were formulated with steeped DDGS as the sole source of CP. The basal mixture contained 0.2% TiO(2) as indigestible marker. Six ileal-cannulated pigs (20 kg BW) were fed the three diets in a replicated 3 × 3 Latin square design to give six replicates per diet. Pigs were fed at 2.8× maintenance energy requirements and had free access to water. In each period, pigs were adjusted to diets for 7 d followed by 2 d for grab fecal and 2 d of 8 h continuous ileal digesta collection. There were no (P > 0.05) treatment and sampling time interaction or treatment effects on pH and lactic concentration. Lactic and acetic acids increased, and pH decreased (P < 0.05) over time points. The AID of CP, NDF, and crude fat and SID of CP were not different (P > 0.05) among treatments. Steeping DDGS with FDE-A had lower (P = 0.01) ATTD of NDF than control but higher (P = 0.001) ATTD of crude fat compared with the control or DDGS steeped with FDE-B. Values for DE content in steeped DDGS were not different (P > 0.05) and amounted to 4,095, 4,039, and 3,974 kcal/kg DM for the control, FDE-A, and FDE-B, respectively. In conclusion, under conditions of the study, steeping DDGS with exogenous enzymes did not improve fiber and energy digestibility.